Team:Virginia/Parts
From 2013.igem.org
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<p><a href="https://2013.igem.org/Team:Virginia/Results">Results</a></p> | <p><a href="https://2013.igem.org/Team:Virginia/Results">Results</a></p> | ||
<p><a href="https://2013.igem.org/Team:Virginia/Modeling">Modeling</a></p> | <p><a href="https://2013.igem.org/Team:Virginia/Modeling">Modeling</a></p> | ||
- | <p><a href="https://2013.igem.org/Team:Virginia/ | + | <p><a href="https://2013.igem.org/Team:Virginia/Software">Software</a></p> |
+ | |||
+ | <p><a href="https://2013.igem.org/Team:Virginia/Chassis_Improvements">Chassis Improvements</a></p></span> | ||
</li> | </li> | ||
<li> | <li> | ||
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</li> | </li> | ||
<li> | <li> | ||
- | + | <span class="title">Human Practices</span> | |
<span class="text"> | <span class="text"> | ||
<p><a href="https://2013.igem.org/Team:Virginia/Human_Practices_Overview">Overview</a></p> | <p><a href="https://2013.igem.org/Team:Virginia/Human_Practices_Overview">Overview</a></p> | ||
- | <p><a href="https://2013.igem.org/Team:Virginia/ | + | <p><a href="https://2013.igem.org/Team:Virginia/Safety Considerations">Safety Considerations</a></p> |
- | <p><a href="https://2013.igem.org/Team:Virginia/ | + | <p><a href="https://2013.igem.org/Team:Virginia/High_School_Education_Series">High School Education Series</a></p> |
- | + | <p><a href="https://2013.igem.org/Team:Virginia/Documentary">Documentary</a></p> | |
+ | <p><a href="https://2013.igem.org/Team:Virginia/Media_Coverage">Media Coverage</a></p> | ||
+ | </span> | ||
</li> | </li> | ||
<li> | <li> | ||
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<p><a href="https://igem.org/Team.cgi?year=2013&team_name=Virginia">Profile</a></p> | <p><a href="https://igem.org/Team.cgi?year=2013&team_name=Virginia">Profile</a></p> | ||
<p><a href="https://2013.igem.org/Team:Virginia/Sponsors">Sponsors</a></p> | <p><a href="https://2013.igem.org/Team:Virginia/Sponsors">Sponsors</a></p> | ||
- | <p><a href="https://2013.igem.org/Team:Virginia/ | + | <p><a href="https://2013.igem.org/Team:Virginia/Attributions">Attributions</a></p></span></li></ul></div></div> |
<div class="body"> | <div class="body"> | ||
- | </ | + | <div id="groupbio"> |
+ | <span>Parts</span> | ||
+ | <p><b>Submitted BioBricks</b></p> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/d/df/Par1.png" width="700"> | ||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/8/82/Par2.png" width="800"> | ||
+ | <p><center>BBa_K1011000 encodes the FtsZ gene. When overexpressed, the FtsZ gene leads to z-ring formation at the cell poles rather than along the cell midline, leading to minicell production.</center></p> | ||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/1/13/Par3.png" width="800"> | ||
+ | <p><center>BBa_S05211 encodes a strong RBS and the GFP gene. This part was produced as an intermediate.</center></p> | ||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/f/f2/Par5.png" width="800"> | ||
+ | <p><center>BBa_S05212 encodes a strong RBS, the GFP gene, and a double terminator. This part was produced as an intermediate, although it is particularly useful as a reporter gene construct for insertion behind the target gene.</center></p> | ||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/a/ae/Par6.png" width="800"> | ||
+ | <p><center>BBa_S05213 encodes an IPTG-inducible promoter, a strong RBS, and the FtsZ gene. This part was produced as an intermediate.</center></p> | ||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2013/8/8f/Par7.png" width="800"> | ||
+ | <p><center>BBa_K1011001 encodes our final construct: an IPTG-inducible promoter, a strong RBS, the FtsZ gene, a strong RBS, the GFP gene, and a double terminator. As overexpression of FtsZ induces minicell formation, GFP serves as a reporter. Use of the ITPG promoter grants control over the level of FtsZ overproduction, which may thus be tuned to optimal minicell production.</center></p> | ||
- | + | </div> | |
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<div class="footer"> | <div class="footer"> | ||
<h3> </h3> | <h3> </h3> |
Latest revision as of 01:54, 29 October 2013
Parts
BBa_K1011000 encodes the FtsZ gene. When overexpressed, the FtsZ gene leads to z-ring formation at the cell poles rather than along the cell midline, leading to minicell production.
BBa_S05211 encodes a strong RBS and the GFP gene. This part was produced as an intermediate.
BBa_S05212 encodes a strong RBS, the GFP gene, and a double terminator. This part was produced as an intermediate, although it is particularly useful as a reporter gene construct for insertion behind the target gene.
BBa_S05213 encodes an IPTG-inducible promoter, a strong RBS, and the FtsZ gene. This part was produced as an intermediate.
BBa_K1011001 encodes our final construct: an IPTG-inducible promoter, a strong RBS, the FtsZ gene, a strong RBS, the GFP gene, and a double terminator. As overexpression of FtsZ induces minicell formation, GFP serves as a reporter. Use of the ITPG promoter grants control over the level of FtsZ overproduction, which may thus be tuned to optimal minicell production.
Submitted BioBricks