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Finalized Protocols: These methods have been designed, performed, and modified appropriately to develop, characterize, and improve our parts. The finalized versions are available below; we recommend them for use by any iGEM team working with our minicell-producing BioBrick (BBa_K1011001).

Developing Protocols: These methods have been designed and are currently being modified appropriately as we conduct our experiments. The most recent versions are available below.