http://2013.igem.org/wiki/index.php?title=Team:WLC-Milwaukee/Overview&feed=atom&action=historyTeam:WLC-Milwaukee/Overview - Revision history2024-03-28T11:51:20ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:WLC-Milwaukee/Overview&diff=230751&oldid=prevMwmortensen at 00:44, 28 September 20132013-09-28T00:44:33Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>How will our project affect the sustainability and cultures of indigenous peoples?</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>How will our project affect the sustainability and cultures of indigenous peoples?</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Dr. Ned Farley: Sustainability is kind of the new topic, and when I say new it’s about ten years old but, it’s new for academics. It’s a new topic of discussion because anthropologists always look at the past more than the future, but what we have realized is that we cannot ignore one for the other. The past, the present, the future, they are all intertwined into culture, and how people use their culture, how they carry it, how they experience it. And so that, kind of in a fast nutshell there, really was a cause in sparking my interest in your proposal and the potential outcomes for that because, and I’ve even brought this up in my anthropology class, the idea of being able to increase rates of sustainability with little to no overhead or capital investment is exactly what humans have looked for since they started writing things down on paper. When we take a look at, for instance, domestication, the domestication of maize [eventually corn] allows people to live in urban environments in the southwest. It is a means of survival; it allows them to grow into large numbers and to flourish….. And so when you came to me yesterday, I think your concern was really great because even though it is not a direct laboratory concern, and that is really where your energies have been spent. You will transform the culture no matter what you do, no matter how hard you try not to, you are always going to affect it. What is awesome about your study is that they could create enough surplus where they could feed themselves. So, now it becomes indigenous again where I live off my herd, and sell it for cash. Right? And that’s powerful. If you can do that without disturbing the balance between biology and culture. You win, hands down. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Dr. Ned Farley: Sustainability is kind of the new topic, and when I say new it’s about ten years old but, it’s new for academics. It’s a new topic of discussion because anthropologists always look at the past more than the future, but what we have realized is that we cannot ignore one for the other. The past, the present, the future, they are all intertwined into culture, and how people use their culture, how they carry it, how they experience it. And so that, kind of in a fast nutshell there, really was a cause in sparking my interest in your proposal and the potential outcomes for that because, and I’ve even brought this up in my anthropology class, the idea of being able to increase rates of sustainability with little to no overhead or capital investment is exactly what humans have looked for since they started writing things down on paper. When we take a look at, for instance, domestication, the domestication of maize [eventually corn] allows people to live in urban environments in the southwest. It is a means of survival; it allows them to grow into large numbers and to flourish….. And so when you came to me yesterday, I think your concern was really great because even though it is not a direct laboratory concern, and that is really where your energies have been spent. You will transform the culture no matter what you do, no matter how hard you try not to, you are always going to affect it. What is awesome about your study is that they could create enough surplus where they could feed themselves. So, now it becomes indigenous again where I live off my herd, and sell it for cash. Right? And that’s powerful. If you can do that without disturbing the balance between biology and culture. You win, hands down. </p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img width="450"src="https://static.igem.org/mediawiki/2013/b/b8/WLC-One_ring_to_secrete_them_all_plasmid_.png" align="right"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><a href="https://static.igem.org/mediawiki/2013/b/b8/WLC-One_ring_to_secrete_them_all_plasmid_.png"></ins><img width="450"src="https://static.igem.org/mediawiki/2013/b/b8/WLC-One_ring_to_secrete_them_all_plasmid_.png" align="right"<ins class="diffchange diffchange-inline">></a</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>The Final Product</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>The Final Product</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This combinatory part is a self contained secretion system that combines a secretion and purification tag, a tripartite pump that is specific to the sec tag, a antinorovirus particle like antibody, Tse2 toxin mediated horizontal gene transfer mechanism. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This combinatory part is a self contained secretion system that combines a secretion and purification tag, a tripartite pump that is specific to the sec tag, a antinorovirus particle like antibody, Tse2 toxin mediated horizontal gene transfer mechanism. </p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>T5cumate repressed Tse2 mediated, antihorizontal transfer mechanism</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>T5cumate repressed Tse2 mediated, antihorizontal transfer mechanism</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> This construct BBa_K1175003 is a modification of the part designed by iGEM2012 Team Trieste it has a T5cumate inducible promoter suppressed by the CymR gene integrated genome into the chassis E.coli Nissle 1917. This part will not be suppressed by CymR in the bacterial cells that have taken up the plasmid.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> This construct BBa_K1175003 is a modification of the part designed by iGEM2012 Team Trieste it has a T5cumate inducible promoter suppressed by the CymR gene integrated genome into the chassis E.coli Nissle 1917. This part will not be suppressed by CymR in the bacterial cells that have taken up the plasmid.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h2>Project Summary</h2></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div class=WordSection1></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal style='text-indent:.5in'>Wisconsin Lutheran College’s 2013 iGEM team</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">has been hard at work to create a secretion mechanism that is safe and</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">customizable. Our way of approaching this is to make use of removable</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">purification and secretion tags that are separated by a single enzyme cut site</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Nhel. This is the cut site that is the insertion point for any coding sequence</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">of interest. Then the induced gene can be secreted from the cytoplasm to the</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">extracellular space via a tripartite secI pump. In order to control a Tse2</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">toxin, a cymR-regulated T5cumate promoter is placed within the plasmid. This</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">will prevent horizontal gene transfer. Essentially, if the plasmid is ejected</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">from the bacteria and picked up by another, the gene producing the toxin will</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">not be suppressed and will kill the cell. The plasmid also encodes a chimeric</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">ompA-monoclonal antibody gene, which when expressed, inserts into the outer</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">membrane. The antibody then selectively binds norovirus within the gut to</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">hinder infection. This has great implications in the way of helping those with</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">viruses in developing nations. This plasmid construct was used to over-express</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">and secrete plant-based, polymer-degrading enzymes bglS, yesZ, and xynA. This</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">will allow us to break down hemicellulose and pectin. These enzymes were</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">isolated from the cellulosome of Bacillus subtilis subtilis 168 to digest plant</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">material. We utilized Escherichia coli Nissle 1917 containing a</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">chromosome-integrated cymR gene to secrete these enzymes in the hope to</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">accentuate dietary uptake for humans and livestock in impoverished countries.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal>&nbsp;</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal>Implications:</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal style='text-indent:.5in'>One of the great potential benefits of this</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">project is provide impoverished people with more access to food. This project</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">would allow a greater array of options for food sources, as well as increase</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">the nutritional value of the food that is already present. The mechanism that</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">this project provides will be extremely helpful to other iGEM teams. This</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">system can be used to secrete any other enzyme or molecule chosen by an iGEM</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">team or even general researchers.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal>&nbsp;</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal>Reception:</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal style='text-indent:.5in'>The intent of this project is to create a</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">product that would aid developing countries, without necessarily changing their</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">cultures, or “westernizing” them. In these countries, nutrition is a major</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">concern. The lack of nutrition in developing countries has the added adverse</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">consequence of increasing chronic diseases. “Nutrition is something easily</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">changeable that has to power to prevent chronic disease, with scientific</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">evidence increasingly supporting the view that alterations in diet have strong</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">effects, both positive and negative, on health throughout life.” Nutrition is</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">the first step to overall health, and therefor, our product could improve the</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">general health of a developing country. Some sources even venture to say that</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">food production is considered an evolutionary advance. Culture is not static,</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">but dynamic. It is the aim of this project to enhance the cultures that it</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">touches.Countries also have varying concerns about GMOs, especially the UK and</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">India. Countries like the United States, Canada, and China would be more likely</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">to accept these products. This product would go through further testing to</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">ensure that it is safe for consumption in order to send to countries that could</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">highly benefit from this product. </p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal>&nbsp;</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal>Future:</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal>Polysaccharides</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">are a convenient energy storage system for many organisms. In order to use the</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">energy, the organism must break down the polysaccharide chain into smaller</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">mono- and disaccharides. Cellulose is one such polysaccharide, an energy</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">storage form for plants. Glucose monomers link together in long rigid chains to</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">form cellulose. The plasmid breaks down plant products that are not cellulose,</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">but similar in structure (hemicellulose and pectin). We have three enzymes to</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">accomplish this: xynA, bglS, and yesZ. The endo-xylanase xynA cleaves xylan</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">polysaccharide chains to form shorter xylan chains. The enzyme yesZ has</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">beta-galactosidase (beta-galacturonidase) activity, cleaving exo-beta-D-galactans</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">to produce single galactose molecules from RG I Pectin. The endo-beta-glucanase</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">(bglS) will cleave internal 1,4 linkages adjacent to 1,3 linkages. A secretion</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">system has been designed to release the enzymes from the bacteria. In future</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">experiments, other enzyme-producing genes will be added to break down plant material</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">further. Cellulase genes are first in line, as cellulose is the largest</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">composition of cell wall. The 2008 Edinburgh team designed the genes required</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">to break down cellulose into glucose. These are an exocellulase, an</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">endocellulase, and a beta-glucosidase. Other enzymes required to fully degrade</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">plant material would be added as well.</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p class=MsoNormal>Overall,</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">this project and the secretion system that it provides will be advantageous and</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">beneficial to future iGEM teams as well as developing countries. </p></ins></div></td></tr>
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</table>Mwmortensenhttp://2013.igem.org/wiki/index.php?title=Team:WLC-Milwaukee/Overview&diff=230461&oldid=prevMwmortensen at 00:37, 28 September 20132013-09-28T00:37:29Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>How will our project affect the sustainability and cultures of indigenous peoples?</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>How will our project affect the sustainability and cultures of indigenous peoples?</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Dr. Ned Farley: Sustainability is kind of the new topic, and when I say new it’s about ten years old but, it’s new for academics. It’s a new topic of discussion because anthropologists always look at the past more than the future, but what we have realized is that we cannot ignore one for the other. The past, the present, the future, they are all intertwined into culture, and how people use their culture, how they carry it, how they experience it. And so that, kind of in a fast nutshell there, really was a cause in sparking my interest in your proposal and the potential outcomes for that because, and I’ve even brought this up in my anthropology class, the idea of being able to increase rates of sustainability with little to no overhead or capital investment is exactly what humans have looked for since they started writing things down on paper. When we take a look at, for instance, domestication, the domestication of maize [eventually corn] allows people to live in urban environments in the southwest. It is a means of survival; it allows them to grow into large numbers and to flourish….. And so when you came to me yesterday, I think your concern was really great because even though it is not a direct laboratory concern, and that is really where your energies have been spent. You will transform the culture no matter what you do, no matter how hard you try not to, you are always going to affect it. What is awesome about your study is that they could create enough surplus where they could feed themselves. So, now it becomes indigenous again where I live off my herd, and sell it for cash. Right? And that’s powerful. If you can do that without disturbing the balance between biology and culture. You win, hands down. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Dr. Ned Farley: Sustainability is kind of the new topic, and when I say new it’s about ten years old but, it’s new for academics. It’s a new topic of discussion because anthropologists always look at the past more than the future, but what we have realized is that we cannot ignore one for the other. The past, the present, the future, they are all intertwined into culture, and how people use their culture, how they carry it, how they experience it. And so that, kind of in a fast nutshell there, really was a cause in sparking my interest in your proposal and the potential outcomes for that because, and I’ve even brought this up in my anthropology class, the idea of being able to increase rates of sustainability with little to no overhead or capital investment is exactly what humans have looked for since they started writing things down on paper. When we take a look at, for instance, domestication, the domestication of maize [eventually corn] allows people to live in urban environments in the southwest. It is a means of survival; it allows them to grow into large numbers and to flourish….. And so when you came to me yesterday, I think your concern was really great because even though it is not a direct laboratory concern, and that is really where your energies have been spent. You will transform the culture no matter what you do, no matter how hard you try not to, you are always going to affect it. What is awesome about your study is that they could create enough surplus where they could feed themselves. So, now it becomes indigenous again where I live off my herd, and sell it for cash. Right? And that’s powerful. If you can do that without disturbing the balance between biology and culture. You win, hands down. </p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/b/b8/WLC-One_ring_to_secrete_them_all_plasmid_.png" align="right"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img <ins class="diffchange diffchange-inline">width="450"</ins>src="https://static.igem.org/mediawiki/2013/b/b8/WLC-One_ring_to_secrete_them_all_plasmid_.png" align="right"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>The Final Product</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>The Final Product</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This combinatory part is a self contained secretion system that combines a secretion and purification tag, a tripartite pump that is specific to the sec tag, a antinorovirus particle like antibody, Tse2 toxin mediated horizontal gene transfer mechanism. </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>This combinatory part is a self contained secretion system that combines a secretion and purification tag, a tripartite pump that is specific to the sec tag, a antinorovirus particle like antibody, Tse2 toxin mediated horizontal gene transfer mechanism. </p></div></td></tr>
</table>Mwmortensenhttp://2013.igem.org/wiki/index.php?title=Team:WLC-Milwaukee/Overview&diff=230446&oldid=prevMwmortensen: Created page with "{{Team:WLC2}} <html><body><h1>Our Project</h1> <h2>Dr. Farley on Indigenous Peoples</h2> <p>How will our project affect the sustainability and cultures of indigenous peoples?</p..."2013-09-28T00:37:12Z<p>Created page with "{{Team:WLC2}} <html><body><h1>Our Project</h1> <h2>Dr. Farley on Indigenous Peoples</h2> <p>How will our project affect the sustainability and cultures of indigenous peoples?</p..."</p>
<p><b>New page</b></p><div>{{Team:WLC2}}<br />
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<html><body><h1>Our Project</h1><br />
<h2>Dr. Farley on Indigenous Peoples</h2><br />
<p>How will our project affect the sustainability and cultures of indigenous peoples?</p><br />
<p>Dr. Ned Farley: Sustainability is kind of the new topic, and when I say new it’s about ten years old but, it’s new for academics. It’s a new topic of discussion because anthropologists always look at the past more than the future, but what we have realized is that we cannot ignore one for the other. The past, the present, the future, they are all intertwined into culture, and how people use their culture, how they carry it, how they experience it. And so that, kind of in a fast nutshell there, really was a cause in sparking my interest in your proposal and the potential outcomes for that because, and I’ve even brought this up in my anthropology class, the idea of being able to increase rates of sustainability with little to no overhead or capital investment is exactly what humans have looked for since they started writing things down on paper. When we take a look at, for instance, domestication, the domestication of maize [eventually corn] allows people to live in urban environments in the southwest. It is a means of survival; it allows them to grow into large numbers and to flourish….. And so when you came to me yesterday, I think your concern was really great because even though it is not a direct laboratory concern, and that is really where your energies have been spent. You will transform the culture no matter what you do, no matter how hard you try not to, you are always going to affect it. What is awesome about your study is that they could create enough surplus where they could feed themselves. So, now it becomes indigenous again where I live off my herd, and sell it for cash. Right? And that’s powerful. If you can do that without disturbing the balance between biology and culture. You win, hands down. </p><br />
<img src="https://static.igem.org/mediawiki/2013/b/b8/WLC-One_ring_to_secrete_them_all_plasmid_.png" align="right"><br />
<h2>The Final Product</h2><br />
<p>This combinatory part is a self contained secretion system that combines a secretion and purification tag, a tripartite pump that is specific to the sec tag, a antinorovirus particle like antibody, Tse2 toxin mediated horizontal gene transfer mechanism. </p><br />
<br />
<h3>Tripartite SecI pump</h3><br />
These are the three essential parts prtD, prtE, prtF, that make up the Type I secretion system from Erwinia chrysanthemi.This part is made of 3 genes: prtD, prtE, and prtF, that constitute a type I Erwinia chrysanthemi secretion system. The Pump is expressed from a strong constitutive promoter, BBa_k206000, and has the translational terminator BBa_B0014. In pSB1C3. Used to secrete proteins containing prtB C-terminal tag. The prtB C-terminal tag is built into the protein generator [BBa_K1175012]. Any protein of interest can be inserted into the protein generator and then secreted when used in conjunction with this secretion system.<br />
<br />
<h3>Secretion and purification tag</h3><br />
This part BBa_K1175012 A composite part formed between BBa_K206000 strong pBAD promoter, BBa_B0034 strong RBS, and BBa_K215001 a purification and secretion tag; a secretion system designed by Washington iGEM team 2009 BBa_K215001. This tag can be used to secrete proteins of interest by inserting them into a specific NheI cut site flanked by C terminus His tag and an N terminus Secretion tag. Both tags can be removed by a TEV protease. The secretion tag is also specific to the tripartite pump. <br />
<br />
<h3>Antinorovirus like particle antibody</h3><br />
This construct BBa_K875004 is designed for the expression of an already described engineered antinorovirus (NoV) monoclonal antibody (mAb 54.6) in fusion with LPP-OmpA. The antibody is expressed in a single chain fragment variable (scFv) format containing light (VL) and heavy (VH) variable domains separeted by a flexible peptide linker. It has already been reported that the scFv 54.6 binds a native recombinant NoV particles (VLPs) and inhibits VLP interaction with cells. LPP-OmpA functions as a leader sequence and an anchor to display the scFv ot the bacterial surface.The construct consistes of T5 Lac Operator (Bba_K875002), ribosomal binding site, LPP-OmpA-scFv 54.6 antinorovirus, Histidine tag (6HIS), Terminator (B0015).<br />
<h3>T5cumate repressed Tse2 mediated, antihorizontal transfer mechanism</h3><br />
This construct BBa_K1175003 is a modification of the part designed by iGEM2012 Team Trieste it has a T5cumate inducible promoter suppressed by the CymR gene integrated genome into the chassis E.coli Nissle 1917. This part will not be suppressed by CymR in the bacterial cells that have taken up the plasmid.</div>Mwmortensen