Team:Washington/96WellPlateAssay

From 2013.igem.org

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1. Pipette 260 ul of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> above to each well being tested and cover with a breathable plate seal.</br>
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1. Pipette 260 ul of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to each well being tested and cover with a breathable plate seal.</br>
2. Start the E. colight app and select the appropriate conditions.</br>
2. Start the E. colight app and select the appropriate conditions.</br>
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.</br>
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.</br>

Revision as of 02:16, 27 September 2013



96 Well Plate Assay
1. Pipette 260 ul of the overnight culture prepared according to the general protocol to each well being tested and cover with a breathable plate seal.
2. Start the E. colight app and select the appropriate conditions.
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.
4. Incubate tablet and 96-well plate on an orbital shaker (RPM?) for 16 hours at 37 oC.
5. Follow the Fluorescent Analysis Protocol

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