Team:Washington/96WellPlateAssay

From 2013.igem.org

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2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br>
2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br>
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.</br>
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.</br>
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4. Incubate tablet and 96-well plate on an orbital shaker 240 RPM for 16 hours at 37C.</br>
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4. Incubate tablet and 96-well plate on an orbital shaker at 240 RPM for 16 hours at 37C.</br>
5. Follow the <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br>
5. Follow the <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br>

Revision as of 22:15, 27 September 2013


96-Well Plate Assay
1. Pipette 260 ul of the overnight culture prepared according to the general protocol to each well being tested and cover with a breathable plate seal.
2. Set the E. colight app with the appropriate conditions.
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.
4. Incubate tablet and 96-well plate on an orbital shaker at 240 RPM for 16 hours at 37C.
5. Follow the Fluorescent Analysis Protocol.