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Team:Washington/96WellPlateAssay
From 2013.igem.org
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| - | <b><u>96 Well Plate Assay</u></b> | + | <b><u>96-Well Plate Assay</u></b> |
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| + | Obtain <a href = "http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=-1&productId=3349439&distype=0&fromSearch=0">96-Well Plate</a>.</br> | ||
1. Pipette 260 ul of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to each well being tested and cover with a breathable plate seal.</br> | 1. Pipette 260 ul of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to each well being tested and cover with a breathable plate seal.</br> | ||
| - | 2. | + | 2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br> |
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.</br> | 3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.</br> | ||
| - | 4. Incubate tablet and 96-well plate on an orbital shaker | + | 4. Incubate tablet and 96-well plate on an orbital shaker at 240 RPM for 16 hours at 37C.</br> |
5. Follow the <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br> | 5. Follow the <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br> | ||
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Latest revision as of 22:27, 27 September 2013
96-Well Plate Assay Obtain 96-Well Plate. 1. Pipette 260 ul of the overnight culture prepared according to the general protocol to each well being tested and cover with a breathable plate seal. 2. Set the E. colight app with the appropriate conditions. 3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape. 4. Incubate tablet and 96-well plate on an orbital shaker at 240 RPM for 16 hours at 37C. 5. Follow the Fluorescent Analysis Protocol.
