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Team:Washington/96WellPlateAssay
From 2013.igem.org
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2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br> | 2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br> | ||
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.</br> | 3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.</br> | ||
| - | 4. Incubate tablet and 96-well plate on an orbital shaker | + | 4. Incubate tablet and 96-well plate on an orbital shaker 240 RPM for 16 hours at 37C.</br> |
5. Follow the <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br> | 5. Follow the <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br> | ||
Revision as of 22:15, 27 September 2013
96-Well Plate Assay 1. Pipette 260 ul of the overnight culture prepared according to the general protocol to each well being tested and cover with a breathable plate seal. 2. Set the E. colight app with the appropriate conditions. 3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape. 4. Incubate tablet and 96-well plate on an orbital shaker 240 RPM for 16 hours at 37C. 5. Follow the Fluorescent Analysis Protocol.
