"
Team:Washington/96WellPlateAssay
From 2013.igem.org
(Difference between revisions)
| Line 10: | Line 10: | ||
<body> | <body> | ||
| + | Obtain <a href = "http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=-1&productId=3349439&distype=0&fromSearch=0">96-Well Plate</a></br>. | ||
1. Pipette 260 ul of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to each well being tested and cover with a breathable plate seal.</br> | 1. Pipette 260 ul of the overnight culture prepared according to the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to each well being tested and cover with a breathable plate seal.</br> | ||
2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br> | 2. <a href = "https://2013.igem.org/Team:Washington/appsetup">Set the E. colight app</a> with the appropriate conditions.</br> | ||
Revision as of 22:26, 27 September 2013
96-Well Plate Assay Obtain 96-Well Plate. 1. Pipette 260 ul of the overnight culture prepared according to the general protocol to each well being tested and cover with a breathable plate seal. 2. Set the E. colight app with the appropriate conditions. 3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape. 4. Incubate tablet and 96-well plate on an orbital shaker at 240 RPM for 16 hours at 37C. 5. Follow the Fluorescent Analysis Protocol.
