Team:Washington/96WellPlateAssay

From 2013.igem.org

Revision as of 22:15, 27 September 2013 by Seo (Talk | contribs)


96-Well Plate Assay
1. Pipette 260 ul of the overnight culture prepared according to the general protocol to each well being tested and cover with a breathable plate seal.
2. Set the E. colight app with the appropriate conditions.
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.
4. Incubate tablet and 96-well plate on an orbital shaker 240 RPM for 16 hours at 37C.
5. Follow the Fluorescent Analysis Protocol.