From 2013.igem.org
1. Pipette 260 ul of the overnight culture prepared according to the general protocol above to each well being tested and cover with a breathable plate seal. (link to general)
2. Start the E. colight app and select the appropriate conditions.
3. Orient the 96-well plate on the tablet such that the wells line up with the appropriate light source on the tablet and secure with tape.
4. Incubate tablet and 96-well plate on an orbital shaker (RPM?) for 16 hours at 37 oC.
5. Follow the Fluorescent Analysis Protocol
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