Team:Washington/BleedThroughTest

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1. Make <a href = "https://2013.igem.org/Team:Washington/M9Media">M9 + Casamino Acid Media</a></br>
 
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2. Add 1g of <a href = "http://www.lifetechnologies.com/order/catalog/product/16520050">Low Melt Agarose</a> per 100ml media. </br>
 
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3. Autoclave the mixture.</br>
 
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4. When solution has cooled down to 42C add antibiotic and innoculate.</br>
 
1. Follow the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to obtain inoculated culture.</br>
1. Follow the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to obtain inoculated culture.</br>

Revision as of 02:57, 27 September 2013



Bleed-Through Test
1. Follow the general protocol to obtain inoculated culture.
2. Follow the protocol for a 96-well Plate setup with these settings.
Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.
Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. (refer to supplemental image)
3. Align the 96-well Plate to the wells on the app and then tape them down securely.

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