Team:Washington/BleedThroughTest

From 2013.igem.org

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1. Follow the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to obtain inoculated culture.</br>
1. Follow the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to obtain inoculated culture.</br>
2. Follow the protocol for a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-well Plate</a> setup with these settings.</br>
2. Follow the protocol for a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-well Plate</a> setup with these settings.</br>
-
Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.</br>
+
- Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.</br>
-
Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. (refer to supplemental image)</br>
+
- Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. (refer to supplemental image)</br>
3. Align the 96-well Plate to the wells on the app and then tape them down securely.
3. Align the 96-well Plate to the wells on the app and then tape them down securely.
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Revision as of 03:00, 27 September 2013



Bleed-Through Test
1. Follow the general protocol to obtain inoculated culture.
2. Follow the protocol for a 96-well Plate setup with these settings.
- Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.
- Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. (refer to supplemental image)
3. Align the 96-well Plate to the wells on the app and then tape them down securely.