"
Team:Washington/BleedThroughTest
From 2013.igem.org
(Difference between revisions)
| (6 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{:Team:Washington/WA_Template}} | {{:Team:Washington/WA_Template}} | ||
| - | |||
| - | |||
<html> | <html> | ||
| - | + | <blockquote> | |
<header> | <header> | ||
<b><u>Bleed-Through Test</u></b> | <b><u>Bleed-Through Test</u></b> | ||
| Line 13: | Line 11: | ||
1. Follow the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to obtain inoculated culture.</br> | 1. Follow the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to obtain inoculated culture.</br> | ||
| - | 2. Follow the protocol for a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96- | + | 2. Follow the protocol for a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate</a> setup with these settings:</br> |
| - | Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.</br> | + | - Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.</br> |
| - | Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. | + | - Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. </br> |
| + | </html> | ||
| + | [[Image:Washington_app_bleed1.jpg|border||300px|left|thumb|]] | ||
| + | <html> | ||
| + | |||
| + | </html> | ||
| + | [[Image:Washington_app_bleed2.jpg|border||300px|left|thumb|]] | ||
| + | <html> | ||
| + | </br> | ||
| + | </br></br></br></br></br></br></br></br></br></br></br></br></br>Note: Addidtional lit wells on the columns serves as replicas. </br> | ||
3. Align the 96-well Plate to the wells on the app and then tape them down securely. | 3. Align the 96-well Plate to the wells on the app and then tape them down securely. | ||
</br> | </br> | ||
</body> | </body> | ||
| - | + | <br> | |
| + | <br> | ||
</html> | </html> | ||
Latest revision as of 21:36, 27 September 2013
Bleed-Through Test 1. Follow the general protocol to obtain inoculated culture. 2. Follow the protocol for a 96-Well Plate setup with these settings: - Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait. - Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait.
Note: Addidtional lit wells on the columns serves as replicas. 3. Align the 96-well Plate to the wells on the app and then tape them down securely.
