Team:Washington/BleedThroughTest

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2. Follow the protocol for a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate</a> setup with these settings:</br>
2. Follow the protocol for a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate</a> setup with these settings:</br>
- Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.</br>
- Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.</br>
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- Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. (refer to supplemental image)</br>
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- Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. </br>
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[[Image:Washington_app_bleed1.jpg|border||300px|left|thumb|]]
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[[Image:Washington_app_bleed2.jpg|border||300px|left|thumb|]]
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</br></br></br></br></br></br></br></br></br></br></br></br></br>Note: Addidtional lit wells on the columns serves as replicas. </br>
3. Align the 96-well Plate to the wells on the app and then tape them down securely.
3. Align the 96-well Plate to the wells on the app and then tape them down securely.
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Latest revision as of 21:36, 27 September 2013


Bleed-Through Test
1. Follow the general protocol to obtain inoculated culture.
2. Follow the protocol for a 96-Well Plate setup with these settings:
- Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.
- Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait.

Washington app bleed1.jpg

Washington app bleed2.jpg















Note: Addidtional lit wells on the columns serves as replicas.
3. Align the 96-well Plate to the wells on the app and then tape them down securely.