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From 2013.igem.org

Bleed-Through Test

1. Follow the general protocol to obtain inoculated culture.
2. Follow the protocol for a 96-well Plate setup with these settings.
Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.
Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait. (refer to supplemental image)
3. Align the 96-well Plate to the wells on the app and then tape them down securely.