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Bleed-Through Test
1. Follow the general protocol to obtain inoculated culture.
2. Follow the protocol for a 96-Well Plate setup with these settings:
- Highlight the first three wells in a row and set all [Color] Wait to 0 except for Green Wait.
- Select every other wells that are not being tested and set all [Color] Wait to 0 except for the Black Wait.

Washington app bleed1.jpg

Washington app bleed2.jpg

Note: Addidtional lit wells on the columns serves as replicas.
3. Align the 96-well Plate to the wells on the app and then tape them down securely.