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Team:Washington/BlinkingTest
From 2013.igem.org
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<b><u>M9 + Casamino Acid Plate</u></b> | <b><u>M9 + Casamino Acid Plate</u></b> | ||
Revision as of 04:39, 27 September 2013
M9 + Casamino Acid Plate 1. Prepare 20 ml of M9 + Casamino Acid Media in a 50 ml falcon tube. 2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest. 3. The blinking timer were set up as follows: - always green light. - 600 millisecond green wait and 400 millisecond black wait. - 125 millisecond green wait and 875 millisecond black wait. - 25 millisecond green wait and 975 millisecond black wait. - 5 millisecond green wait and 995 millisecond black wait. - always dark. 4. Follow steps of the 96-Well Plate Assay.
