Team:Washington/BlinkingTest

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4. Follow steps of the <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate Assay</a>.
4. Follow steps of the <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate Assay</a>.
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Revision as of 04:40, 27 September 2013


M9 + Casamino Acid Plate 1. Prepare 20 ml of M9 + Casamino Acid Media in a 50 ml falcon tube.
2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest.
3. The blinking timer were set up as follows:
- always green light.
- 600 millisecond green wait and 400 millisecond black wait.
- 125 millisecond green wait and 875 millisecond black wait.
- 25 millisecond green wait and 975 millisecond black wait.
- 5 millisecond green wait and 995 millisecond black wait.
- always dark.
4. Follow steps of the 96-Well Plate Assay.