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Team:Washington/FluorescentAnalysisProtocol
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2. GFP expression levels are analyzed on a spectramax M5e plate reader (bottom read) with an excitation of 480 nm and emission at 520nm. Set the plate reader to automix the samples for 5 seconds prior to analysis.</br> | 2. GFP expression levels are analyzed on a spectramax M5e plate reader (bottom read) with an excitation of 480 nm and emission at 520nm. Set the plate reader to automix the samples for 5 seconds prior to analysis.</br> | ||
3. GFP expression levels were normalized against cell density by dividing the relative fluorescence unit values by OD600 data.</br> | 3. GFP expression levels were normalized against cell density by dividing the relative fluorescence unit values by OD600 data.</br> | ||
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Latest revision as of 04:39, 27 September 2013
Fluorescent Analysis Protocol 1. Remove 200 ul culture from each well, and pipette to a 96-well black, clear-bottom plate for analysis. 2. GFP expression levels are analyzed on a spectramax M5e plate reader (bottom read) with an excitation of 480 nm and emission at 520nm. Set the plate reader to automix the samples for 5 seconds prior to analysis. 3. GFP expression levels were normalized against cell density by dividing the relative fluorescence unit values by OD600 data.
