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From 2013.igem.org

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Heat shock protocol (approximate time 1hrs)

1. Remove and thaw (on ice) chemically competent cells, either 1.5ml tube or PCR tube.

Lowercase letters list:

1. 1.5mL tube aliquots of cells contain approx. 205uL cells
2. Pcr tubes aliquots contain 50uls
2. Add 1-5µl DNA to 40-50µl of competent cells.
3. ncubate on ice for 5 minutes (for higher transformation efficiency, do 30 min)
4. Heat shock cells for 45 seconds at 42°C
5. Let cells stand on ice for 2 minutes (or longer)
6. Add 200uL2-1mL of LB and shake for 30 min at 37°C (for higher transformation efficiency, do 1 hr). This is called recovery.
7. Plate 200uL cells on appropriate antibiotic plate1 and grow O/N in 37°C incubator

1. Preheat plates in 37C before for better results (labeling and cell absorption).
3. If using 300ul pcr tubes, omit shaking, incubate 37C.

NOTE: Do not use XL10 Gold Cells with Chlor Plates