Team:Washington/LightIntensityTest

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1. Follow the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to obtain an overnight culture listed above.</br>
1. Follow the <a href = "https://2013.igem.org/Team:Washington/GeneralProtocol">general protocol</a> to obtain an overnight culture listed above.</br>
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2. Follow the protocol to run a 96-well test, setting up the E. colight app as follows, wells are set to a varying intensity from 100% to 0%, decreasing by 20% for every other well. For a clear setup, check the supplemental image. (link and picture relevant to it)</br>
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2. Follow the protocol to run a <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-well test</a>, setting up the E. colight app as follows, wells are set to a varying intensity from 100% to 0%, decreasing by 20% for every other well. For a clear setup, check the supplemental image. (link and picture relevant to it)</br>
3. Run <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br>
3. Run <a href = "https://2013.igem.org/Team:Washington/FluorescentAnalysisProtocol">Fluorescent Analysis Protocol</a>.</br>

Revision as of 03:51, 27 September 2013



Light Intensity Test
1. Follow the general protocol to obtain an overnight culture listed above.
2. Follow the protocol to run a 96-well test, setting up the E. colight app as follows, wells are set to a varying intensity from 100% to 0%, decreasing by 20% for every other well. For a clear setup, check the supplemental image. (link and picture relevant to it)
3. Run Fluorescent Analysis Protocol.

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