Team:Washington/M9Media

From 2013.igem.org

(Difference between revisions)
(Created page with "{{:Team:Washington/WA_Template}} <html> <header> <b><u>M9 Media + Casamino Acid Recipe</u></b> </header> <body> For 1L of media mix: </br></br> 200 ml 2 x M9 salts</br> 30 ...")
Line 12: Line 12:
<body>
<body>
For 1L of media mix: </br></br>
For 1L of media mix: </br></br>
-
200 ml 2 x M9 salts</br>
+
200 ml 5X M9 salts</br>
30 ml 10 mg/ml thiamine hydrochloride</br>
30 ml 10 mg/ml thiamine hydrochloride</br>
10 ml 40% glycerol </br>
10 ml 40% glycerol </br>

Revision as of 01:42, 27 September 2013



M9 Media + Casamino Acid Recipe
For 1L of media mix:

200 ml 5X M9 salts
30 ml 10 mg/ml thiamine hydrochloride
10 ml 40% glycerol
20 ml 10% casamino acids
20 ml 0.1M MgSO4
200 μl 0.5M CaCl2
dH2O until 1L

Filter sterilize the mixture into sterile bottle.

Retrieved from "http://2013.igem.org/Team:Washington/M9Media"