Team:Washington/M9Media

From 2013.igem.org

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For 1L of media mix: </br></br>
For 1L of media mix: </br></br>
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200 ml <li><a href = "https://2013.igem.org/Team:Washington/M9Salts">5X M9 Salts</a></li></br>
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200 ml <a href = "https://2013.igem.org/Team:Washington/M9Salts">5X M9 Salts</a></br>
30 ml 10 mg/ml thiamine hydrochloride</br>
30 ml 10 mg/ml thiamine hydrochloride</br>
10 ml 40% glycerol </br>
10 ml 40% glycerol </br>

Revision as of 01:45, 27 September 2013



M9 Media + Casamino Acid Recipe
For 1L of media mix:

200 ml 5X M9 Salts
30 ml 10 mg/ml thiamine hydrochloride
10 ml 40% glycerol
20 ml 10% casamino acids
20 ml 0.1M MgSO4
200 μl 0.5M CaCl2
dH2O until 1L

Filter sterilize the mixture into sterile bottle.

Retrieved from "http://2013.igem.org/Team:Washington/M9Media"