Team:Washington/M9Media

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<b><u>M9 Media + Casamino Acid Recipe</u></b>
<b><u>M9 Media + Casamino Acid Recipe</u></b>
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For 1L of media mix: </br></br>
For 1L of media mix: </br></br>
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200 ml 5X M9 salts</br>
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200 ml <a href = "https://2013.igem.org/Team:Washington/M9Salts">5X M9 Salts</a></br>
30 ml 10 mg/ml thiamine hydrochloride</br>
30 ml 10 mg/ml thiamine hydrochloride</br>
10 ml 40% glycerol </br>
10 ml 40% glycerol </br>

Latest revision as of 04:36, 27 September 2013


M9 Media + Casamino Acid Recipe
For 1L of media mix:

200 ml 5X M9 Salts
30 ml 10 mg/ml thiamine hydrochloride
10 ml 40% glycerol
20 ml 10% casamino acids
20 ml 0.1M MgSO4
200 μl 0.5M CaCl2
dH2O until 1L

Filter sterilize the mixture into sterile bottle.