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Team:Washington/Protocols
From 2013.igem.org
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<li><a href = "https://2013.igem.org/Team:Washington/HEAT_SHOCK_CHEM_TRANS">6) Heat shock/chemical competent transformation</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/HEAT_SHOCK_CHEM_TRANS">6) Heat shock/chemical competent transformation</a></li> | ||
<li><a href = "https://2013.igem.org/Team:Washington/COLONY_PCR_GREEN">7) Colony PCR with Green taq</a> Miniprep (stocks can be made from this culture - add 1ml extra)</li> | <li><a href = "https://2013.igem.org/Team:Washington/COLONY_PCR_GREEN">7) Colony PCR with Green taq</a> Miniprep (stocks can be made from this culture - add 1ml extra)</li> | ||
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<li><a href = "https://2013.igem.org/Team:Washington/MAKING_GLYCEROL_FROZEN_STOCKS">9) Making Glycerol Frozen Stocks</a></li> | <li><a href = "https://2013.igem.org/Team:Washington/MAKING_GLYCEROL_FROZEN_STOCKS">9) Making Glycerol Frozen Stocks</a></li> | ||
</ul> | </ul> | ||
Revision as of 03:45, 27 September 2013
Workflow:
- iGEM Vector Information
- General Cloning Strategy
- 1) Isolation of Plasmid DNA (miniprep)
- 2) General PCR Protocol (also see PCR GoTaq - product (30-200ng/ ul) Check on gel PCR Purification and/or Dpnl Digest
- 3) General Digestion Protocol Check on gel PCR Purification or Heat Inactivation (check enzyme temp and time, usually 80C for 20min)
- 4) Agarose Gel Electrophoresis
- 5) General Ligation Protocol (Don’t forget background control plates) Heat Inactivation (optional - up to 10 fold increase) - 65° for 10 minutes
- 6) Heat shock/chemical competent transformation
- 7) Colony PCR with Green taq Miniprep (stocks can be made from this culture - add 1ml extra)
- 9) Making Glycerol Frozen Stocks
Light Sensor Protocols
Light Testing Media:
