Team:XMU-China/wetlab journal

From 2013.igem.org

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title=Team:XMU_Software/css&action=raw&amp;ctype=text/css"/>
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<div class="M1_exp_stepInfo" title="0808 BL21(DE3) Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s0">
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<button class="M1_exp_step_name_button"><input value="0808 BL21(DE3) Gfp
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choose plasmid (B)
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(pSB1C3) aiia(pSB3T5)" class="M1_exp_step_name"></button> <span
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</th> </tr>
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class="M1_exp_step_time">2013/9/18 10:50:50--Chun Tang</span> </div>
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<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
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<td class="M1_exp_item_time" id="ib1s0i0">2013/9/25 20:38:47</td>
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</td> <td style="display: none;">
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choose plasmid (C) </th>
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</tr> <tr>
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class="C3_td"><input value="" class="C3_input"></td>
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name
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</th>
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<td class="C3_td"><input value="" class="C3_input"></td>
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length
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value="" class="C3_input"></td> </tr>
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</tbody></table>
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</td> </tr> <tr>
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<td class="C3_td" colspan="2"> <input
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type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
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name="#i0s7i0"> <input type="button"
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class="C8_button" value="if you don't need the plasmid (C) , double click here"
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name="#i0s7i0" style="display: none;"> </td>
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</tr> </tbody></table></td> </tr>
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<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
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id="ib0s7i1">2013/8/15 23:44:36</td> <td class="M1_exp_item_content">
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<table id="i0s7i1" class="C4">
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<tbody><tr> <th colspan="3">Plasmid
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Extraction</th> <td rowspan="9"
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class="C4_td_textarea"> <textarea
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class="C3_textarea" id="i0s7i19">
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</textarea></td> </tr>
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<tr> <td class="C3_td">      </td>
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<th class="C3_td">A<sub>260/280</sub></th>
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<th class="C3_td">ng/μL</th>
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</tr> <tr> <td
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class="C3_td" align="center">(A)</td> <td
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class="C3_td"><input value="1.83" class="C3_input"></td>
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<td class="C3_td"><input value="59.7" class="C3_input"></td>
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</tr> <tr>
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<td class="C3_td" align="center">(B)</td> <td
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class="C3_td"><input value="1.80" class="C3_input"></td>
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<td class="C3_td"><input value="29.3" class="C3_input"></td>
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</tr> <tr class="C4_tr_C" style="display: none;">
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<td class="C3_td" align="center">(C)</td>
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<td class="C3_td"><input value="
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" class="C3_input"></td> <td
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class="C3_td"><input value="10" class="C3_input"></td>
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</tr> <tr> <th
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class="C3_td" colspan="3"> <button
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class="C8_button" id="i0s7i112" name="#i0s7i1" value="3">
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Evaluate
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</button> </th>
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</tr> <tr style="display: none;">
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<table class="C3"> <tbody><tr>
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<td
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class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td>
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-
 
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<td class="C3_td">great</td>
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<td class="C3_td"><input value="" class="C3_input"></td>
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</tr>
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<tr>
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-
 
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<td class="C3_td"><input value="" class="C3_input"></td>
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-
 
+
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<td class="C3_td">fine</td>
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<td class="C3_td"><input value="" class="C3_input"></td>
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-
 
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</tr>
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-
 
+
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<tr>
+
-
 
+
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<td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td>
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-
 
+
-
<td class="C3_td" rowspan="2">not good</td>
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<td class="C3_td"><input
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-
 
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value="" class="C3_input"></td>
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-
 
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</tr> <tr>
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<td class="C3_td"><input value=""
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-
 
+
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class="C3_input"></td> </tr>
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-
 
+
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</tbody></table>
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-
 
+
-
</td> </tr>
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-
 
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-
</tbody></table></td> </tr> <tr class="M1_exp_stepItem">
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+
-
<td class="M1_exp_item_time" id="ib0s7i2">2013/8/15 23:43:57</td>
+
-
 
+
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<td class="M1_exp_item_content"> <table id="i0s7i2"
+
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+
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class="C8"> <tbody><tr> <th
+
-
 
+
-
colspan="2" class="C8_td_title"> Start to
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-
 
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Ligate Plasmids </th> </tr>
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-
 
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-
<tr> <td>
+
-
 
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<table class="C8"> <tbody><tr>
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-
 
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-
<th colspan="3" class="C8_td_title">
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-
 
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-
choose plasmid (A)
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-
 
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</th> </tr>
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-
 
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<tr>
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-
 
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-
<th rowspan="2" class="C3_td">
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-
 
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-
plasmid </th>
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-
 
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-
<th class="C3_td">
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-
 
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-
name
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-
 
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</th> <td class="C3_td"><input
+
-
 
+
-
value="AI2M19" class="C3_input"></td>
+
-
 
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</tr> <tr>
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-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
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-
 
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</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
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-
 
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<tr>
+
-
 
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-
<th class="C3_td">
+
-
 
+
-
type </th>
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-
 
+
-
<td class="C3_td" colspan="2"><input value="rbs-tetr-tt-
+
-
 
+
-
ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
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part-only
+
-
 
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</th> <th class="C3_td">
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-
 
+
-
sequence
+
-
 
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-
</th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
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-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
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-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
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-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
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+
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</tr> <tr>
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<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
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</th> <td class="C3_td"><input
+
-
 
+
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value="1767" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
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-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
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</th> <th class="C3_td">
+
-
 
+
-
name
+
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</th> <td
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+
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class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
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</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
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+
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</th> <td class="C3_td"><input
+
-
 
+
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value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
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choose plasmid (B)
+
-
 
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</th> </tr>
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-
 
+
-
<tr> <th
+
-
 
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-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
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-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
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</th> <td class="C3_td"><input
+
-
 
+
-
value="18A" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
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</th> <td class="C3_td"><input
+
-
 
+
-
value="2013 P5 18A" class="C3_input"></td>
+
-
 
+
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</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input
+
-
 
+
-
value="pcon" class="C3_input"></td>
+
-
 
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-
</tr> <tr>
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-
 
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-
<th rowspan="2" class="C3_td">
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-
 
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-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
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</th>
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-
 
+
-
<td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td>
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-
 
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</tr>
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-
 
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-
<tr> <th class="C3_td">
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-
 
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-
length
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-
 
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</th>
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-
 
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-
<td class="C3_td"><input value="35" class="C3_input"></td>
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</tr> <tr>
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-
 
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-
<th rowspan="2" class="C3_td">
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-
 
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backbone
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-
 
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-
</th> <th
+
-
 
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-
class="C3_td"> name
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-
 
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</th>
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-
 
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-
<td class="C3_td"><input value="psb1a2" class="C3_input"></td>
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-
 
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</tr> <tr>
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-
 
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<th class="C3_td">
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-
 
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length
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-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td style="display: none;">
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th
+
-
 
+
-
colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="2"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i0s7i2"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i0s7i2" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s7i3">2013/8/15 23:47:13</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s7i3" class="C4">
+
-
 
+
-
<tbody><tr> <th colspan="3">Plasmid
+
-
 
+
-
Extraction</th> <td rowspan="9"
+
-
 
+
-
class="C4_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s7i39">
+
-
 
+
-
</textarea></td> </tr>
+
-
 
+
-
<tr> <td class="C3_td">      </td>
+
-
 
+
-
<th class="C3_td">A<sub>260/280</sub></th>
+
-
 
+
-
<th class="C3_td">ng/μL</th>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" align="center">(A)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.82" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="101.6" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td" align="center">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.78" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="45.1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C4_tr_C" style="display: none;">
+
-
 
+
-
<td class="C3_td" align="center">(C)</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" colspan="3">
+
-
 
+
-
<button class="C8_button" id="i0s7i312" name="#i0s7i3">
+
-
 
+
-
Evaluate
+
-
 
+
-
</button> </th>
+
-
 
+
-
</tr> <tr style="display: none;">
+
-
 
+
-
<td colspan="3">
+
-
 
+
-
<table class="C3">
+
-
 
+
-
<tbody><tr> <td
+
-
 
+
-
class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td>
+
-
 
+
-
<td class="C3_td">great</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td">fine</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td>
+
-
 
+
-
<td class="C3_td" rowspan="2">not good</td>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
</tbody></table></td> </tr> <tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib0s7i4">2013/8/15 23:52:24</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table class="C5" id="i0s7i4">
+
-
 
+
-
<tbody><tr> <th colspan="3" id="i0s7i429"
+
-
 
+
-
class="C5_th_C">Extract plasmid</th> <td rowspan="10"
+
-
 
+
-
class="C3_td_textarea"> <textarea class="C3_textarea"
+
-
 
+
-
id="i0s7i40"> </textarea>
+
-
 
+
-
</td> </tr> <tr> <th
+
-
 
+
-
class="C5_canvas_td" colspan="3" id="i0s7i430"> <div
+
-
 
+
-
class="C5_canvas_matrix"> </div>
+
-
 
+
-
<div class="C5_TEXT1">EX</div> <div
+
-
 
+
-
class="C5_TEXT2">SP</div> <input type="button"
+
-
 
+
-
class="C5_canvas_button1" id="i0s7i41" name="#i0s7i4" value="18A (B)">
+
-
 
+
-
<input type="button" class="C5_canvas_button2" id="i0s7i42" name="#i0s7i4"
+
-
 
+
-
value="AI2M19 (A)"> <input type="button"
+
-
 
+
-
class="C5_canvas_button3" id="i0s7i43" name="#i0s7i4" value="18A (B)">
+
-
 
+
-
</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th>
+
-
 
+
-
<th class="C3_td">(A)</th> <th
+
-
 
+
-
class="C3_td">(B)</th> <th class="C3_td_C5_C"
+
-
 
+
-
style="display: none;">(C)</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Plasmid</th>
+
-
 
+
-
<td class="C3_td"><input value="62" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="62" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="10"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Eco</em>R I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Xba</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Spe</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Pst</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">H<sub>2</sub>0 /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="18" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="18" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="18"
+
-
 
+
-
class="C3_input"></td> <input type="hidden" id="i0s7i426"
+
-
 
+
-
value="1"> <input type="hidden" id="i0s7i427"
+
-
 
+
-
value="0"> <input type="hidden" id="i0s7i428"
+
-
 
+
-
value="1"> </tr> </tbody></table></td>
+
-
 
+
-
</tr> <tr class="M1_exp_stepItem"> <td
+
-
 
+
-
class="M1_exp_item_time" id="ib0s7i5">2013/8/15 23:54:43</td> <td
+
-
 
+
-
class="M1_exp_item_content"> <table id="i0s7i5" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i0s7i50" class="C1_graph"
+
-
 
+
-
src="http://trysomething-block.stor.sinaapp.com/t1i0s9i613.8.15 18A12M19,18AI.jpg"><br>
+
-
 
+
-
<form id="i0s7i54" target="M7" action="img.php" method="post"  
+
enctype="multipart/form-data"></form> <input name="img"  
enctype="multipart/form-data"></form> <input name="img"  
-
type="file" id="i0s7i52" form="i0s7i54" class="C1_button1" required="required" disabled="">
+
type="file" id="i1s0i02" form="i1s0i04" class="C1_button1" required="required" disabled="">
-
<input name="pname" type="hidden" value="t1" form="i0s7i54">
+
<input name="pname" type="hidden" value="t14" form="i1s0i04">
-
<input name="id" type="hidden" value="i0s7i5" form="i0s7i54">
+
<input name="id" type="hidden" value="i1s0i0" form="i1s0i04">
-
<input name="oldgraph" type="hidden" id="i0s7i55" form="i0s7i54"  
+
<input name="oldgraph" type="hidden" id="i1s0i05" form="i1s0i04"  
-
value="t1i0s9i613.8.15 18A12M19,18AI.jpg"> <input id="i0s7i56"  
+
value="t14i1s0i5808.gif"> <input id="i1s0i06" type="submit"  
-
type="submit" value="submit" form="i0s7i54" class="C1_button2" disabled="">
+
value="submit" form="i1s0i04" class="C1_button2" disabled=""> <div
-
<div id="i0s7i53" class="M1_exp_img"></div> </td>
+
id="i1s0i03" class="M1_exp_img"></div> </td> <td
-
<td class="C1_td_text"> <textarea id="i0s7i51"  
+
class="C1_td_text"> <textarea id="i1s0i01" class="C1_text">8th
-
class="C1_text">A12M19,18AI</textarea> <br>
+
August
 +
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
 +
Bacteria:BL21(DE3)
 +
Condition: No flowing medium culture supply. Cells trap in the micro pools. Without
-
</td> </tr> </tbody></table> </td> </tr>
+
antibiotic putting in.
 +
Total Obserbation Time:200mins
 +
Analysis:
 +
At the beginning, the population of cells is small and the fluorescence degree is as the
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
same. Minutes later, more cells grew as well as stronger fluorescence was detected. From
-
id="ib0s7i6">2013/8/15 23:55:52</td> <td class="M1_exp_item_content">
+
then on, we observed weak oscillation of the fluorescence intensity.
 +
</textarea> <br> </td> </tr>
-
<table id="i0s7i6" class="C6"> <tbody><tr>
+
</tbody></table> </td> </tr> </tbody></table>
-
<th class="C3_td_title" colspan="5">Electrophoretogram and
+
<div class="M1_exp_stepInfo" title="0809 BL21(DE3)  Gfp(pSB1C3) aiia(pSB3T5)"  
-
recover</th> <td rowspan="10" class="C6_td_textarea">
+
id="sb1s1"> <button class="M1_exp_step_name_button"><input value="0809
-
<textarea class="C3_textarea" id="i0s7i66">
+
BL21(DE3)  Gfp(pSB1C3) aiia(pSB3T5)" class="M1_exp_step_name"></button>
-
</textarea> </td>
+
<span class="M1_exp_step_time">2013/9/18 10:51:01--Chun Tang</span> </div>
-
</tr> <tr> <th
+
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
-
 
+
-
colspan="5" class="C3_td"> Analyse
+
-
 
+
-
Electrophoretogram </th>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct0s7i6">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">A<sub>260/280</sub></th> <th
+
-
 
+
-
class="C3_td">ng/μL</th> <th class="C3_td">bp</th>
+
-
 
+
-
<td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Correct" class="C6_button" name="0s7i6">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Correct0s7i6"> <td class="C3_td"
+
-
 
+
-
nowrap="nowrap">(A)</td> <td class="C3_td"><input
+
-
 
+
-
value="1.53" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="5.4" class="C6_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="1767" class="C6_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct0s7i6">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.59" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="6.3" class="C6_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="2114" class="C6_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct0s7i6" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap">(C)</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Wrong0s7i6" style="display: none;">
+
-
 
+
-
<td class="C3_td" colspan="4" rowspan="3">
+
-
 
+
-
Possible reason:<br> 1. The
+
-
 
+
-
validity or contamination of gel extraction kit and DD water.<br>
+
-
 
+
-
2. The validity of enzyme and buffer<br>
+
-
 
+
-
3. Incomplete dissolution of agar gel<br>
+
-
 
+
-
</td> <td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Wrong" class="C6_button" name="0s7i6">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Wrong0s7i6" style="display: none;"> </tr>
+
-
 
+
-
<tr class="mdoelC5Wrong0s7i6" style="display: none;">
+
-
 
+
-
</tr> <tr class="mdoelC5Wrong0s7i6" style="display: none;">
+
-
 
+
-
</tr> </tbody></table> <input
+
-
 
+
-
type="hidden" id="i0s7i67" value="1"></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s7i7">2013/8/15 23:56:29</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s7i7" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s7i70"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i0s7i726">A
+
-
 
+
-
(insert) /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="5.9620413327709825" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="6" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
id="i0s7i727">B (vector) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="2.0379586672290175" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<th class="C3_td">ligase /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s7i8">2013/8/16 23:59:03</td> <td
+
-
 
+
-
class="M1_exp_item_content"><textarea id="i0s7i8" class="C0" rows="1">TRANSFORMATION AND DO
+
-
 
+
-
THE LIGATION AGAIN</textarea></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
id="ib0s7i9">2013/8/17 23:59:37</td> <td class="M1_exp_item_content">
+
<td class="M1_exp_item_time" id="ib1s1i0">2013/9/25 20:42:38</td>
-
<table id="i0s7i9" class="C1"> <tbody><tr> <td class="C1_td">
+
<td class="M1_exp_item_content"> <table id="i1s1i0" class="C1"> <tbody><tr>
-
<img id="i0s7i90" class="C1_graph" src="http://trysomething-
+
<td class="C1_td"> <img id="i1s1i00"  
-
block.stor.sinaapp.com/t1i0s9i1013.8.16 18AI-18AI2M19 1 2 3 4 18AI M ZX.jpg"><br>
+
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s1i3809.gif"><br>
-
<form id="i0s7i94" target="M7" action="img.php" method="post"  
+
<form id="i1s1i04" target="M7" action="img.php" method="post"  
enctype="multipart/form-data"></form> <input name="img"  
enctype="multipart/form-data"></form> <input name="img"  
-
type="file" id="i0s7i92" form="i0s7i94" class="C1_button1" required="required" disabled="">
+
type="file" id="i1s1i02" form="i1s1i04" class="C1_button1" required="required" disabled="">
-
<input name="pname" type="hidden" value="t1" form="i0s7i94">
+
<input name="pname" type="hidden" value="t14" form="i1s1i04">
-
<input name="id" type="hidden" value="i0s7i9" form="i0s7i94">
+
<input name="id" type="hidden" value="i1s1i0" form="i1s1i04">
-
<input name="oldgraph" type="hidden" id="i0s7i95" form="i0s7i94"  
+
<input name="oldgraph" type="hidden" id="i1s1i05" form="i1s1i04"  
-
value="t1i0s9i1013.8.16 18AI-18AI2M19 1 2 3 4 18AI M ZX.jpg">
+
value="t14i1s1i3809.gif"> <input id="i1s1i06" type="submit"
-
<input id="i0s7i96" type="submit" value="submit" form="i0s7i94" class="C1_button2"  
+
value="submit" form="i1s1i04" class="C1_button2" disabled=""> <div
-
disabled=""> <div id="i0s7i93" class="M1_exp_img"></div>
+
id="i1s1i03" class="M1_exp_img"></div> </td> <td
-
</td> <td class="C1_td_text">
+
class="C1_td_text"> <textarea id="i1s1i01"
-
<textarea id="i0s7i91" class="C1_text">18AI-AI2M19 1 2 3 4 18AI M ZX
+
class="C1_text">choose the photo yo9th August
-
  WWWWC</textarea>
+
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
 +
Bacteria:BL21(DE3)
 +
Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic
-
<br> </td> </tr> </tbody></table> </td>
+
putting in.
 +
Total Obserbation Time:140mins
 +
Analysis:
 +
At the beginning, the population of cells is small and the fluorescence degree is as the
-
</tr> <tr class="M1_exp_stepItem"> <td
+
same. Minutes later, more cells grew but the population is still not large enough to observe
-
class="M1_exp_item_time" id="ib0s7i10">2013/8/19 00:07:27</td> <td  
+
convictive fluorescence intensity changing.
 +
</textarea> <br> </td> </tr>
-
class="M1_exp_item_content"> <table id="i0s7i10" class="C1"> <tbody><tr>
+
</tbody></table> </td> </tr> </tbody></table>
-
<td class="C1_td"> <img id="i0s7i100" class="C1_graph"  
+
<div class="M1_exp_stepInfo" title="0825 DH5a Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s2">
-
src="http://trysomething-block.stor.sinaapp.com/t1i0s9i1113.8.18 PC 18A-AI2M19 M
+
<button class="M1_exp_step_name_button"><input value="0825 DH5a Gfp(pSB1C3)
-
18A.jpg"><br> <form id="i0s7i104" target="M7" action="img.php"
+
aiia(pSB3T5)" class="M1_exp_step_name"></button> <span
-
method="post" enctype="multipart/form-data"></form> <input
+
class="M1_exp_step_time">2013/9/18 10:51:41--Chun Tang</span> </div>
-
 
+
-
name="img" type="file" id="i0s7i102" form="i0s7i104" class="C1_button1" required="required"
+
-
 
+
-
disabled=""> <input name="pname" type="hidden" value="t1"
+
-
 
+
-
form="i0s7i104"> <input name="id" type="hidden"
+
-
 
+
-
value="i0s7i10" form="i0s7i104"> <input name="oldgraph"
+
-
 
+
-
type="hidden" id="i0s7i105" form="i0s7i104" value="t1i0s9i1113.8.18 PC 18A-AI2M19 M
+
-
 
+
-
18A.jpg"> <input id="i0s7i106" type="submit" value="submit"
+
-
 
+
-
form="i0s7i104" class="C1_button2" disabled=""> <div id="i0s7i103"
+
-
 
+
-
class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i0s7i101" class="C1_text">
+
-
 
+
-
18A-AI2M19 M 18A
+
-
</textarea> <br> </td>
+
-
 
+
-
</tr> </tbody></table> </td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s7i11">2013/8/19 00:09:02</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s7i11" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s7i110"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i0s7i1126">A
+
-
 
+
-
(insert) /μL</th> <td class="C3_td"><input value="0"
+
-
 
+
-
class="C3_input"></td> <td class="C3_td"><input value="7"
+
-
 
+
-
class="C3_input"></td> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i0s7i1127">B
+
-
 
+
-
(vector) /μL</th> <td class="C3_td"><input value="0"
+
-
 
+
-
class="C3_input"></td> <td class="C3_td"><input value="1"
+
-
 
+
-
class="C3_input"></td> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td">ligase /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s7i12">2013/8/20 00:13:32</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s7i12" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i0s7i120" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s9i1313.8.19 18A-AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN
+
-
 
+
-
(123456).jpg"><br> <form id="i0s7i124" target="M7"
+
-
 
+
-
action="img.php" method="post" enctype="multipart/form-data"></form>
+
-
 
+
-
<input name="img" type="file" id="i0s7i122" form="i0s7i124" class="C1_button1"
+
-
 
+
-
required="required" disabled=""> <input name="pname"
+
-
 
+
-
type="hidden" value="t1" form="i0s7i124"> <input name="id"
+
-
 
+
-
type="hidden" value="i0s7i12" form="i0s7i124"> <input
+
-
 
+
-
name="oldgraph" type="hidden" id="i0s7i125" form="i0s7i124" value="t1i0s9i1313.8.19 18A-
+
-
 
+
-
AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN(123456).jpg">
+
-
 
+
-
<input id="i0s7i126" type="submit" value="submit" form="i0s7i124" class="C1_button2"
+
-
 
+
-
disabled=""> <div id="i0s7i123" class="M1_exp_img"></div>
+
-
 
+
-
</td> <td class="C1_td_text">
+
-
 
+
-
<textarea id="i0s7i121" class="C1_text">18A-AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN
+
-
 
+
-
(123456)
+
-
W  W  W                                      WCCCCC                       
+
-
 
+
-
  WCWWWC</textarea> <br> </td>
+
-
 
+
-
</tr> </tbody></table> </td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="18A-AI2M19" id="sb0s8">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="18A-AI2M19"
+
-
 
+
-
class="M1_exp_step_name"></button> <span
+
-
 
+
-
class="M1_exp_step_time">2013/8/20 00:14:31--Ruosang</span> </div>
+
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
-
<td class="M1_exp_item_time" id="ib0s8i0">2013/8/20 00:14:48</td>
+
<td class="M1_exp_item_time" id="ib1s2i0">2013/9/25 20:45:15</td>
-
<td class="M1_exp_item_content"> <table id="i0s8i0" class="C8">
+
<td class="M1_exp_item_content"> <table id="i1s2i0" class="C1"> <tbody><tr>
-
<tbody><tr> <th colspan="2"  
+
<td class="C1_td"> <img id="i1s2i00"  
-
class="C8_td_title"> Start to Ligate Plasmids
+
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s2i0825.gif"><br>
-
</th> </tr>
+
<form id="i1s2i04" target="M7" action="img.php" method="post"
-
<tr> <td>
+
enctype="multipart/form-data"></form> <input name="img"
-
<table class="C8"> <tbody><tr>
+
type="file" id="i1s2i02" form="i1s2i04" class="C1_button1" required="required" disabled="">
-
<th colspan="3" class="C8_td_title">
+
<input name="pname" type="hidden" value="t14" form="i1s2i04">
-
choose plasmid (A)
+
<input name="id" type="hidden" value="i1s2i0" form="i1s2i04">
-
</th> </tr>
+
<input name="oldgraph" type="hidden" id="i1s2i05" form="i1s2i04"
-
<tr> <th
+
value="t14i1s2i0825.gif"> <input id="i1s2i06" type="submit"
-
rowspan="2" class="C3_td">
+
value="submit" form="i1s2i04" class="C1_button2" disabled=""> <div
-
plasmid </th>
+
id="i1s2i03" class="M1_exp_img"></div> </td> <td
-
<th class="C3_td">
+
class="C1_td_text"> <textarea id="i1s2i01" class="C1_text">25th
-
name
+
August
 +
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
 +
Bacteria:DH5a
 +
Condition: No flowing medium culture supply. Cells trap in the micro pools. With antibiotic
-
</th> <td class="C3_td"><input
+
putting in.
 +
Total Obserbation Time:165mins
 +
Analysis:
 +
At the beginning, the population of cells is small and the fluorescence degree is as the
-
value="18A" class="C3_input"></td>
+
same. Minutes later, more cells grew and we detect stronger fluorescence degree. From then
-
</tr> <tr>
+
on, the fluorescence degree kept in almost the same intensity.
 +
</textarea> <br> </td> </tr>
-
<th class="C3_td">
+
</tbody></table> </td> </tr> </tbody></table>
-
location
+
<div class="M1_exp_stepInfo" title="0831 DH5a Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s3">
-
</th> <td class="C3_td"><input  
+
<button class="M1_exp_step_name_button"><input value="0831 DH5a Gfp(pSB1C3)
-
value="2013 P5 18A" class="C3_input"></td>
+
aiia(pSB3T5)" class="M1_exp_step_name"></button> <span
-
</tr> <tr>
+
class="M1_exp_step_time">2013/9/18 10:52:00--Chun Tang</span> </div>
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input
+
-
 
+
-
value="pcon" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="35" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (B) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="AI2M19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
+
-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
+
-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
+
-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="1767" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td style="display: none;">
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="2"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i0s8i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i0s8i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s8i1">2013/8/20 00:15:21</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s8i1" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i0s8i10" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s10i113.8.19 18A-AI2M19 1 2.jpg"><br>
+
-
 
+
-
<form id="i0s8i14" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i0s8i12"
+
-
 
+
-
form="i0s8i14" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i0s8i14">
+
-
 
+
-
<input name="id" type="hidden" value="i0s8i1" form="i0s8i14">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i0s8i15" form="i0s8i14" value="t1i0s10i113.8.19
+
-
 
+
-
18A-AI2M19 1 2.jpg"> <input id="i0s8i16" type="submit"
+
-
 
+
-
value="submit" form="i0s8i14" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i0s8i13" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i0s8i11"
+
-
 
+
-
class="C1_text">choose the photo you want to submit</textarea> <br>
+
-
 
+
-
</td> </tr> </tbody></table> </td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s8i2">2013/8/20 00:16:34</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s8i2" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i0s8i20" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s10i213.8.19 18A.jpg"><br> <form
+
-
 
+
-
id="i0s8i24" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i0s8i22"
+
-
 
+
-
form="i0s8i24" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i0s8i24">
+
-
 
+
-
<input name="id" type="hidden" value="i0s8i2" form="i0s8i24">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i0s8i25" form="i0s8i24" value="t1i0s10i213.8.19
+
-
 
+
-
18A.jpg"> <input id="i0s8i26" type="submit" value="submit"
+
-
 
+
-
form="i0s8i24" class="C1_button2" disabled=""> <div id="i0s8i23"
+
-
 
+
-
class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i0s8i21"
+
-
 
+
-
class="C1_text">choose the photo you want to submit</textarea> <br>
+
-
 
+
-
</td> </tr> </tbody></table> </td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s8i3">2013/8/20 00:16:50</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s8i3" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s8i30"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i0s8i326">A
+
-
 
+
-
(vector) /μL</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> <td class="C3_td"><input value="1"
+
-
 
+
-
class="C3_input"></td> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i0s8i327">B
+
-
 
+
-
(insert) /μL</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> <td class="C3_td"><input value="7"
+
-
 
+
-
class="C3_input"></td> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td">ligase /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:  
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="18A-AI2M19" id="sb0s9">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="18A-AI2M19"
+
-
 
+
-
class="M1_exp_step_name"></button> <span
+
-
 
+
-
class="M1_exp_step_time">2013/8/21 00:17:26--Ruosang</span> </div>
+
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
-
<td class="M1_exp_item_time" id="ib0s9i0">2013/8/21 00:17:45</td>
+
<td class="M1_exp_item_time" id="ib1s3i0">2013/9/25 20:46:04</td>
-
<td class="M1_exp_item_content"> <table id="i0s9i0" class="C8">
+
<td class="M1_exp_item_content"> <table id="i1s3i0" class="C1"> <tbody><tr>
-
<tbody><tr> <th colspan="2"  
+
<td class="C1_td"> <img id="i1s3i00"  
-
class="C8_td_title"> Start to Ligate Plasmids
+
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s3i0831.gif"><br>
-
</th> </tr>
+
<form id="i1s3i04" target="M7" action="img.php" method="post"  
-
 
+
-
<tr> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="18A" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2013 P5 18A" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input
+
-
 
+
-
value="pcon" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="35" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (B) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="18A" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2013 P5 18A" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input
+
-
 
+
-
value="pcon" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="35" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td style="display: none;">
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th
+
-
 
+
-
colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="2"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i0s9i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i0s9i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s9i1">2013/8/21 00:18:04</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s9i1" class="C4">
+
-
 
+
-
<tbody><tr> <th colspan="3">Plasmid
+
-
 
+
-
Extraction</th> <td rowspan="9"
+
-
 
+
-
class="C4_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s9i19">
+
-
 
+
-
</textarea></td> </tr>
+
-
 
+
-
<tr> <td class="C3_td">      </td>
+
-
 
+
-
<th class="C3_td">A<sub>260/280</sub></th>
+
-
 
+
-
<th class="C3_td">ng/μL</th>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" align="center">(A)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.87" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="89.6" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td" align="center">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.68" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="107.2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C4_tr_C" style="display: none;">
+
-
 
+
-
<td class="C3_td" align="center">(C)</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" colspan="3">
+
-
 
+
-
<button class="C8_button" id="i0s9i112" name="#i0s9i1">
+
-
 
+
-
Evaluate
+
-
 
+
-
</button> </th>
+
-
 
+
-
</tr> <tr style="display: none;">
+
-
 
+
-
<td colspan="3">
+
-
 
+
-
<table class="C3">
+
-
 
+
-
<tbody><tr> <td
+
-
 
+
-
class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td>
+
-
 
+
-
<td class="C3_td">great</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td">fine</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td>
+
-
 
+
-
<td class="C3_td" rowspan="2">not good</td>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
</tbody></table></td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="18O-AI2M19" id="sb0s10"> <button
+
-
 
+
-
class="M1_exp_step_name_button"><input value="18O-AI2M19" class="M1_exp_step_name"></button>
+
-
 
+
-
<span class="M1_exp_step_time">2013/8/22 00:18:33--Ruosang</span>
+
-
 
+
-
</div> <table class="M1_exp_stepBoard"> <tbody><tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s10i0">2013/8/22 00:18:50</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s10i0" class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="2" class="C8_td_title">
+
-
 
+
-
Start to Ligate Plasmids </th>
+
-
 
+
-
</tr> <tr> <td>
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th
+
-
 
+
-
colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="18O" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2013 P5 18O" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="pcon" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> backbone
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a2"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
</tbody></table> </td>
+
-
 
+
-
<td> <table class="C8">
+
-
 
+
-
<tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (B) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="AI2M19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
+
-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
+
-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
+
-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="1767" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td style="display: none;">
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="2"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i0s10i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i0s10i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s10i1">2013/8/22 00:19:07</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s10i1" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i0s10i10" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s12i113.8.22 18o ai2m19 18o1 s d  18o2s.jpg"><br>
+
-
 
+
-
<form id="i0s10i14" target="M7" action="img.php" method="post"  
+
enctype="multipart/form-data"></form> <input name="img"  
enctype="multipart/form-data"></form> <input name="img"  
-
type="file" id="i0s10i12" form="i0s10i14" class="C1_button1" required="required"  
+
type="file" id="i1s3i02" form="i1s3i04" class="C1_button1" required="required" disabled="">
-
disabled=""> <input name="pname" type="hidden" value="t1"  
+
<input name="pname" type="hidden" value="t14" form="i1s3i04">
-
form="i0s10i14"> <input name="id" type="hidden"  
+
<input name="id" type="hidden" value="i1s3i0" form="i1s3i04">
-
value="i0s10i1" form="i0s10i14"> <input name="oldgraph"  
+
<input name="oldgraph" type="hidden" id="i1s3i05" form="i1s3i04"  
-
type="hidden" id="i0s10i15" form="i0s10i14" value="t1i0s12i113.8.22 18o ai2m19 18o1 s d 
+
value="t14i1s3i0831.gif"> <input id="i1s3i06" type="submit"  
-
18o2s.jpg"> <input id="i0s10i16" type="submit" value="submit"  
+
value="submit" form="i1s3i04" class="C1_button2" disabled=""> <div
-
form="i0s10i14" class="C1_button2" disabled=""> <div id="i0s10i13"
+
id="i1s3i03" class="M1_exp_img"></div> </td> <td
-
class="M1_exp_img"></div> </td> <td
+
class="C1_td_text"> <textarea id="i1s3i01" class="C1_text">31th
-
class="C1_td_text"> <textarea id="i0s10i11" class="C1_text">18o
+
Auguest
 +
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
 +
Bacteria:DH5a
 +
Condition: Flowing medium culture supply. Cells could flow into different traps. With
-
ai2m19 18o1 s d  18o2s
+
antibiotic putting in.
-
C</textarea> <br> </td>
+
Total Obserbation Time:80mins
 +
Analysis:
 +
At the beginning, the population of cell was large enough to get valid fluorescence degree.
-
</tr> </tbody></table> </td> </tr> <tr
+
And from then on , we observed no fluorescence intensity changing.
 +
</textarea> <br> </td> </tr>
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
</tbody></table> </td> </tr> </tbody></table>
-
id="ib0s10i2">2013/8/22 00:20:05</td> <td class="M1_exp_item_content">
+
<div class="M1_exp_stepInfo" title="0912 DH5a Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s4">
-
<table class="C5" id="i0s10i2"> <tbody><tr>
+
<button class="M1_exp_step_name_button"><input value="0912 DH5a Gfp(pSB1C3)
-
<th colspan="3" id="i0s10i229" class="C5_th_C">Extract plasmid</th>
+
aiia(pSB3T5)" class="M1_exp_step_name"></button> <span  
-
 
+
-
<td rowspan="10" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s10i20"> </textarea>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C5_canvas_td" colspan="3" id="i0s10i230"> <div
+
-
 
+
-
class="C5_canvas_matrix"> </div>
+
-
 
+
-
<div class="C5_TEXT1">EX</div> <div
+
-
 
+
-
class="C5_TEXT2">SP</div> <input type="button"
+
-
 
+
-
class="C5_canvas_button1" id="i0s10i21" name="#i0s10i2" value="18O (A)">
+
-
 
+
-
<input type="button" class="C5_canvas_button2" id="i0s10i22" name="#i0s10i2"
+
-
 
+
-
value="AI2M19 (B)"> <input type="button"
+
-
 
+
-
class="C5_canvas_button3" id="i0s10i23" name="#i0s10i2" value="18O (A)">
+
-
 
+
-
</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th>
+
-
 
+
-
<th class="C3_td">(A)</th>
+
-
 
+
-
<th class="C3_td">(B)</th> <th class="C3_td_C5_C"
+
-
 
+
-
style="display: none;">(C)</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Plasmid</th>
+
-
 
+
-
<td class="C3_td"><input value="40" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="50" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="10"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Eco</em>R I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Xba</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Spe</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Pst</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">H<sub>2</sub>0 /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="40" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="30" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="18"
+
-
 
+
-
class="C3_input"></td> <input type="hidden" id="i0s10i226"
+
-
 
+
-
value="0"> <input type="hidden" id="i0s10i227"
+
-
 
+
-
value="1"> <input type="hidden" id="i0s10i228"
+
-
 
+
-
value="0"> </tr> </tbody></table></td>
+
-
 
+
-
</tr> <tr class="M1_exp_stepItem"> <td
+
-
 
+
-
class="M1_exp_item_time" id="ib0s10i3">2013/8/22 00:20:33</td> <td
+
-
 
+
-
class="M1_exp_item_content"> <table id="i0s10i3" class="C7">
+
-
 
+
-
<tbody><tr> <th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation
+
-
 
+
-
</th> <td rowspan="8" class="C3_td_textarea">
+
-
 
+
-
<textarea class="C3_textarea" id="i0s10i30">
+
-
 
+
-
</textarea> </td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3"
+
-
 
+
-
class="C7_blur_1"></td> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <td class="C3_td"></td>
+
-
 
+
-
<th class="C3_td">Recommend</th> <th
+
-
 
+
-
class="C3_td">Actual use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i0s10i326">A
+
-
 
+
-
(vector) /μL</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> <td class="C3_td"><input value="1"
+
-
 
+
-
class="C3_input"></td> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i0s10i327">B
+
-
 
+
-
(insert) /μL</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> <td class="C3_td"><input value="7"
+
-
 
+
-
class="C3_input"></td> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td">ligase /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="18O AND 18A" id="sb0s11">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="18O AND 18A"
+
-
 
+
-
class="M1_exp_step_name"></button> <span  
+
-
class="M1_exp_step_time">2013/8/23 00:21:17--Ruosang</span> </div>
+
class="M1_exp_step_time">2013/9/18 10:52:31--Chun Tang</span> </div>
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
-
<td class="M1_exp_item_time" id="ib0s11i0">2013/8/23 00:21:30</td>
+
<td class="M1_exp_item_time" id="ib1s4i0">2013/9/25 20:47:42</td>
-
<td class="M1_exp_item_content"> <table id="i0s11i0" class="C8">
+
<td class="M1_exp_item_content"> <table id="i1s4i0" class="C1"> <tbody><tr>
-
<tbody><tr> <th colspan="2"  
+
<td class="C1_td"> <img id="i1s4i00"  
-
class="C8_td_title"> Start to Ligate Plasmids
+
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s4i0912.gif"><br>
-
</th> </tr>
+
<form id="i1s4i04" target="M7" action="img.php" method="post"  
-
 
+
-
<tr> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="18A" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2013 P5 18A" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input
+
-
 
+
-
value="pcon" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="35" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (B) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="18O" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2013 P5 18O" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input
+
-
 
+
-
value="pcon" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td style="display: none;">
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th
+
-
 
+
-
colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="2"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i0s11i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i0s11i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s11i1">2013/8/23 00:21:48</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s11i1" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i0s11i10" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s13i113.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"><br>
+
-
 
+
-
<form id="i0s11i14" target="M7" action="img.php" method="post"  
+
enctype="multipart/form-data"></form> <input name="img"  
enctype="multipart/form-data"></form> <input name="img"  
-
type="file" id="i0s11i12" form="i0s11i14" class="C1_button1" required="required"  
+
type="file" id="i1s4i02" form="i1s4i04" class="C1_button1" required="required" disabled="">
-
disabled=""> <input name="pname" type="hidden" value="t1"  
+
<input name="pname" type="hidden" value="t14" form="i1s4i04">
-
form="i0s11i14"> <input name="id" type="hidden"  
+
<input name="id" type="hidden" value="i1s4i0" form="i1s4i04">
-
value="i0s11i1" form="i0s11i14"> <input name="oldgraph"  
+
<input name="oldgraph" type="hidden" id="i1s4i05" form="i1s4i04"  
-
type="hidden" id="i0s11i15" form="i0s11i14" value="t1i0s13i113.8.23 18o-ai2m19 1 2 3 M 18A 1
+
value="t14i1s4i0912.gif"> <input id="i1s4i06" type="submit"  
-
2.jpg"> <input id="i0s11i16" type="submit" value="submit"  
+
value="submit" form="i1s4i04" class="C1_button2" disabled=""> <div
-
form="i0s11i14" class="C1_button2" disabled=""> <div id="i0s11i13"
+
id="i1s4i03" class="M1_exp_img"></div> </td> <td
-
class="M1_exp_img"></div> </td> <td
+
class="C1_td_text"> <textarea id="i1s4i01" class="C1_text">12th
-
class="C1_td_text"> <textarea id="i0s11i11"
+
September
 +
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
 +
Bacteria:DH5a
 +
Condition: Flowing medium culture supply. Cells could flow into different traps. With
-
class="C1_text">18o-ai2m19 1 2 3 M 18A 1 2
+
antibiotic putting in.
-
WCC            C W</textarea>
+
Total Obserbation Time:180mins
 +
Analysis:
 +
At the beginning, the population of cell was large enough to get valid fluorescence degree.
-
<br> </td> </tr> </tbody></table> </td>
+
From then on ,there was  a weak decrease of the fluorescence degee.
 +
</textarea> <br> </td> </tr>
-
</tr> </tbody></table> <div class="M1_exp_stepInfo"
+
</tbody></table> </td> </tr> </tbody></table>
-
title="18OAI2M19" id="sb0s12"> <button
+
<div class="M1_exp_stepInfo" title="0915  BL21 Gfp(pSB1C3) aiia(pSB3T5)" id="sb1s5">
-
class="M1_exp_step_name_button"><input value="18OAI2M19" class="M1_exp_step_name"></button>
+
<button class="M1_exp_step_name_button"><input value="0915  BL21 Gfp(pSB1C3)
-
<span class="M1_exp_step_time">2013/8/23 00:23:10--Ruosang</span>
+
aiia(pSB3T5)" class="M1_exp_step_name"></button> <span  
-
</div> <table class="M1_exp_stepBoard"> <tbody><tr
+
class="M1_exp_step_time">2013/9/18 10:53:02--Chun Tang</span> </div>
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s12i0">2013/8/23 00:23:21</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s12i0" class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
Start to Ligate Plasmids </th>
+
-
 
+
-
</tr> <tr> <td>
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th
+
-
 
+
-
colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="18OAI2M19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> part-only
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaa
+
-
 
+
-
gtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcc
+
-
 
+
-
tacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctt
+
-
 
+
-
tagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacat
+
-
 
+
-
ttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatt
+
-
 
+
-
atatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacaccta
+
-
 
+
-
ctactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattg
+
-
 
+
-
atcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgata
+
-
 
+
-
gtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
+
-
 
+
-
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattg
+
-
 
+
-
acatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatta
+
-
 
+
-
gatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcac
+
-
 
+
-
tactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
+
-
 
+
-
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
+
-
 
+
-
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
+
-
 
+
-
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattg
+
-
 
+
-
aagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcc
+
-
 
+
-
acacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcat
+
-
 
+
-
ggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
+
-
 
+
-
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="1808" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1c3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2070" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (B) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="18OAI2M19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> part-only
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaa
+
-
 
+
-
gtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcc
+
-
 
+
-
tacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctt
+
-
 
+
-
tagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacat
+
-
 
+
-
ttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatt
+
-
 
+
-
atatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacaccta
+
-
 
+
-
ctactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattg
+
-
 
+
-
atcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgata
+
-
 
+
-
gtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
+
-
 
+
-
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattg
+
-
 
+
-
acatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatta
+
-
 
+
-
gatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcac
+
-
 
+
-
tactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
+
-
 
+
-
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
+
-
 
+
-
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
+
-
 
+
-
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattg
+
-
 
+
-
aagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcc
+
-
 
+
-
acacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcat
+
-
 
+
-
ggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
+
-
 
+
-
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="1808" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1c3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2070" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="18OAI2M19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> part-only
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaa
+
-
 
+
-
gtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcc
+
-
 
+
-
tacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctt
+
-
 
+
-
tagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacat
+
-
 
+
-
ttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatt
+
-
 
+
-
atatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacaccta
+
-
 
+
-
ctactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattg
+
-
 
+
-
atcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgata
+
-
 
+
-
gtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
+
-
 
+
-
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattg
+
-
 
+
-
acatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatta
+
-
 
+
-
gatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcac
+
-
 
+
-
tactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
+
-
 
+
-
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
+
-
 
+
-
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
+
-
 
+
-
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattg
+
-
 
+
-
aagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcc
+
-
 
+
-
acacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcat
+
-
 
+
-
ggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
+
-
 
+
-
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="1808" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1c3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2070" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="3">
+
-
 
+
-
<input type="button" class="C8_button" value="if you need another plasmid (C) ,
+
-
 
+
-
double click here" name="#i0s12i0"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you don't need the plasmid (C) , double click
+
-
 
+
-
here" name="#i0s12i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s12i1">2013/8/23 00:24:04</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s12i1" class="C4">
+
-
 
+
-
<tbody><tr> <th colspan="3">Plasmid
+
-
 
+
-
Extraction</th> <td rowspan="10"
+
-
 
+
-
class="C4_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s12i19">
+
-
 
+
-
</textarea></td> </tr>
+
-
 
+
-
<tr> <td class="C3_td">      </td>
+
-
 
+
-
<th class="C3_td">A<sub>260/280</sub></th>
+
-
 
+
-
<th class="C3_td">ng/μL</th>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" align="center">(A)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.84" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="27.9" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td" align="center">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.76" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="29.3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C4_tr_C">
+
-
 
+
-
<td class="C3_td" align="center">(C)</td>
+
-
 
+
-
<td class="C3_td"><input value="1.68" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="166.3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3">
+
-
 
+
-
<button class="C8_button" id="i0s12i112" name="#i0s12i1">
+
-
 
+
-
Evaluate
+
-
 
+
-
</button> </th>
+
-
 
+
-
</tr> <tr style="display: none;">
+
-
 
+
-
<td colspan="3">
+
-
 
+
-
<table class="C3"> <tbody><tr>
+
-
 
+
-
<td
+
-
 
+
-
class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td>
+
-
 
+
-
<td class="C3_td">great</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td">fine</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td>
+
-
 
+
-
<td class="C3_td" rowspan="2">not good</td>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
</tbody></table></td> </tr> <tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib0s12i2">2013/8/23 00:24:54</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i0s12i2" class="C1">
+
-
 
+
-
<tbody><tr> <td class="C1_td"> <img
+
-
 
+
-
id="i0s12i20" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s14i213.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"><br>
+
-
 
+
-
<form id="i0s12i24" target="M7" action="img.php" method="post"
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"
+
-
 
+
-
type="file" id="i0s12i22" form="i0s12i24" class="C1_button1" required="required"
+
-
 
+
-
disabled=""> <input name="pname" type="hidden" value="t1"
+
-
 
+
-
form="i0s12i24"> <input name="id" type="hidden"
+
-
 
+
-
value="i0s12i2" form="i0s12i24"> <input name="oldgraph"
+
-
 
+
-
type="hidden" id="i0s12i25" form="i0s12i24" value="t1i0s14i213.8.23 18o-ai2m19 1 2 3 M 18A 1
+
-
 
+
-
2.jpg"> <input id="i0s12i26" type="submit" value="submit"
+
-
 
+
-
form="i0s12i24" class="C1_button2" disabled=""> <div id="i0s12i23"
+
-
 
+
-
class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i0s12i21"
+
-
 
+
-
class="C1_text">WRONG AND USE THE PLASMID TO DO THE DIGESTION</textarea>
+
-
 
+
-
<br> </td> </tr> </tbody></table> </td>
+
-
 
+
-
</tr> <tr class="M1_exp_stepItem"> <td
+
-
 
+
-
class="M1_exp_item_time" id="ib0s12i3">2013/8/23 00:25:28</td> <td
+
-
 
+
-
class="M1_exp_item_content"><textarea id="i0s12i3" class="C0" rows="1">FAILD</textarea></td>
+
-
 
+
-
</tr> </tbody></table> <div
+
-
 
+
-
class="M1_exp_stepInfo" title="18O-AI2M19" id="sb0s13"> <button
+
-
 
+
-
class="M1_exp_step_name_button"><input value="18O-AI2M19" class="M1_exp_step_name"></button>
+
-
 
+
-
<span class="M1_exp_step_time">2013/8/23 00:26:59--Ruosang</span>
+
-
 
+
-
</div> <table class="M1_exp_stepBoard"> <tbody><tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i0">2013/8/23 00:27:17</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s13i0" class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="2" class="C8_td_title">
+
-
 
+
-
Start to Ligate Plasmids </th>
+
-
 
+
-
</tr> <tr> <td>
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th
+
-
 
+
-
colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="18O" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="2013 P5 18O" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="pcon" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> backbone
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a2"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
</tbody></table> </td>
+
-
 
+
-
<td> <table class="C8">
+
-
 
+
-
<tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (B) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="AI2M19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type
+
-
 
+
-
</th> <td class="C3_td"
+
-
 
+
-
colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
+
-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
+
-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
+
-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="1767" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td style="display: none;">
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="2"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i0s13i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i0s13i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i1">2013/8/23 00:28:07</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table class="C5" id="i0s13i1"> <tbody><tr>
+
-
 
+
-
<th colspan="3" id="i0s13i129" class="C5_th_C">Extract plasmid</th>
+
-
 
+
-
<td rowspan="10" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s13i10"> </textarea>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C5_canvas_td" colspan="3" id="i0s13i130"> <div
+
-
 
+
-
class="C5_canvas_matrix"> </div>
+
-
 
+
-
<div class="C5_TEXT1">EX</div> <div
+
-
 
+
-
class="C5_TEXT2">SP</div> <input type="button"
+
-
 
+
-
class="C5_canvas_button1" id="i0s13i11" name="#i0s13i1" value="18O (A)">
+
-
 
+
-
<input type="button" class="C5_canvas_button2" id="i0s13i12" name="#i0s13i1"
+
-
 
+
-
value="AI2M19 (B)"> <input type="button"
+
-
 
+
-
class="C5_canvas_button3" id="i0s13i13" name="#i0s13i1" value="18O (A)">
+
-
 
+
-
</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th>
+
-
 
+
-
<th class="C3_td">(A)</th>
+
-
 
+
-
<th class="C3_td">(B)</th> <th class="C3_td_C5_C"
+
-
 
+
-
style="display: none;">(C)</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Plasmid</th>
+
-
 
+
-
<td class="C3_td"><input value="30" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="10"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Eco</em>R I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Xba</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Spe</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Pst</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">H<sub>2</sub>0 /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="50" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="80" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="18"
+
-
 
+
-
class="C3_input"></td> <input type="hidden" id="i0s13i126"
+
-
 
+
-
value="0"> <input type="hidden" id="i0s13i127"
+
-
 
+
-
value="1"> <input type="hidden" id="i0s13i128"
+
-
 
+
-
value="0"> </tr> </tbody></table></td>
+
-
 
+
-
</tr> <tr class="M1_exp_stepItem"> <td
+
-
 
+
-
class="M1_exp_item_time" id="ib0s13i2">2013/8/23 00:29:20</td> <td
+
-
 
+
-
class="M1_exp_item_content"> <table id="i0s13i2" class="C6">
+
-
 
+
-
<tbody><tr> <th class="C3_td_title"
+
-
 
+
-
colspan="5">Electrophoretogram and recover</th> <td
+
-
 
+
-
rowspan="10" class="C6_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s13i26"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th colspan="5" class="C3_td">
+
-
 
+
-
Analyse Electrophoretogram </th>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct0s13i2">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">A<sub>260/280</sub></th> <th
+
-
 
+
-
class="C3_td">ng/μL</th> <th class="C3_td">bp</th>
+
-
 
+
-
<td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Correct" class="C6_button" name="0s13i2">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Correct0s13i2"> <td class="C3_td"
+
-
 
+
-
nowrap="nowrap">(A)</td> <td class="C3_td"><input
+
-
 
+
-
value="1.54" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="6.4" class="C6_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C6_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct0s13i2">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="4" class="C6_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="1767" class="C6_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct0s13i2" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap">(C)</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Wrong0s13i2" style="display:
+
-
 
+
-
none;"> <td class="C3_td" colspan="4" rowspan="3">
+
-
 
+
-
Possible reason:<br>
+
-
 
+
-
1. The validity or contamination of gel extraction kit and DD water.<br>
+
-
 
+
-
2. The validity of enzyme and buffer<br>
+
-
 
+
-
3. Incomplete dissolution of agar gel<br>
+
-
 
+
-
</td> <td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Wrong" class="C6_button" name="0s13i2">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Wrong0s13i2" style="display: none;"> </tr>
+
-
 
+
-
<tr class="mdoelC5Wrong0s13i2" style="display: none;">
+
-
 
+
-
</tr> <tr class="mdoelC5Wrong0s13i2" style="display: none;">
+
-
 
+
-
</tr> </tbody></table> <input
+
-
 
+
-
type="hidden" id="i0s13i27" value="1"></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i3">2013/8/23 00:29:43</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s13i3" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s13i30"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i0s13i326">A
+
-
 
+
-
(vector) /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="1.574910516447929" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="2" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
id="i0s13i327">B (insert) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="6.425089483552071" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="4" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<th class="C3_td">ligase /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="3" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i4">2013/8/24 00:33:05</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s13i4" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i0s13i40" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s15i10180 8.24.jpg"><br> <form
+
-
 
+
-
id="i0s13i44" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i0s13i42"
+
-
 
+
-
form="i0s13i44" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i0s13i44">
+
-
 
+
-
<input name="id" type="hidden" value="i0s13i4" form="i0s13i44">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i0s13i45" form="i0s13i44" value="t1i0s15i10180
+
-
 
+
-
8.24.jpg"> <input id="i0s13i46" type="submit" value="submit"
+
-
 
+
-
form="i0s13i44" class="C1_button2" disabled=""> <div id="i0s13i43"
+
-
 
+
-
class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i0s13i41" class="C1_text">ALL
+
-
 
+
-
WRONG</textarea> <br> </td>
+
-
 
+
-
</tr> </tbody></table> </td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i5">2013/8/25 00:30:23</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s13i5" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i0s13i50"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i0s13i526">A
+
-
 
+
-
(vector) /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="1.574910516447929" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="2" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
id="i0s13i527">B (insert) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="6.425089483552071" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="6" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<th class="C3_td">ligase /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i6">2013/8/25 00:30:39</td> <td
+
-
 
+
-
class="M1_exp_item_content"><textarea id="i0s13i6" class="C0"
+
-
 
+
-
rows="1">TRANSFORMATION</textarea></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i7">2013/8/26 00:33:48</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s13i7" class="C9">
+
-
 
+
-
<tbody><tr> <th colspan="6">Template and
+
-
 
+
-
Primers</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3"> choose plasmid
+
-
 
+
-
</th> <th class="C3_td" rowspan="4"> primer1
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th> <td
+
-
 
+
-
class="C3_td"><input value="V1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="18OAI2M19 (9)"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td"> location </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> direction
+
-
 
+
-
</th> <td class="C3_td">
+
-
 
+
-
<select id="i0s13i74" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
position </th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> part-only
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaa
+
-
 
+
-
gtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcc
+
-
 
+
-
tacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctt
+
-
 
+
-
tagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacat
+
-
 
+
-
ttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcatt
+
-
 
+
-
atatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacaccta
+
-
 
+
-
ctactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattg
+
-
 
+
-
atcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgata
+
-
 
+
-
gtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
+
-
 
+
-
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattg
+
-
 
+
-
acatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatta
+
-
 
+
-
gatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcac
+
-
 
+
-
tactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
+
-
 
+
-
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
+
-
 
+
-
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
+
-
 
+
-
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattg
+
-
 
+
-
aagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcc
+
-
 
+
-
acacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcat
+
-
 
+
-
ggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
+
-
 
+
-
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td" rowspan="4">
+
-
 
+
-
primer2 </th>
+
-
 
+
-
<th class="C3_td"> name
+
-
 
+
-
</th> <td class="C3_td"><input value="V2"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
length </th> <td
+
-
 
+
-
class="C3_td"><input value="1808" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">sequence</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td">name</th> <td class="C3_td"><input
+
-
 
+
-
value="psb1c3" class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">direction</th> <td class="C3_td">
+
-
 
+
-
<select id="i0s13i712" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">length</th>
+
-
 
+
-
<td class="C3_td"><input value="2070" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i8">2013/8/26 00:34:19</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s13i8" class="C10">
+
-
 
+
-
<tbody><tr> <th colspan="3">PCR-Reaction</th>
+
-
 
+
-
<td rowspan="9" class="C4_td_textarea">
+
-
 
+
-
<textarea class="C3_textarea" id="i0s13i80">
+
-
 
+
-
</textarea>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Reaction /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="25" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="2.5"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap">dNTP (
+
-
 
+
-
Mixture（2.5 mM each） ) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="4" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="V1" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="1"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap"><input
+
-
 
+
-
value="V2" class="C10_system_input"></th> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">Template /μL <select
+
-
 
+
-
id="i0s13i810">   <option
+
-
 
+
-
value="colony">colony</option>  
+
-
 
+
-
<option value="plasmid">plasmid</option>
+
-
 
+
-
</select> </th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">rTaq /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0.25" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td">H<sub>2</sub>O /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="15.25" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i9">2013/8/26 00:31:30</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s13i9" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i0s13i90" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s15i9180 8.26 18o-2m19 连接鉴定.jpg"><br>
+
-
 
+
-
<form id="i0s13i94" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i0s13i92"
+
-
 
+
-
form="i0s13i94" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i0s13i94">
+
-
 
+
-
<input name="id" type="hidden" value="i0s13i9" form="i0s13i94">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i0s13i95" form="i0s13i94" value="t1i0s15i9180 8.26
+
-
 
+
-
18o-2m19 连接鉴定.jpg"> <input id="i0s13i96" type="submit"
+
-
 
+
-
value="submit" form="i0s13i94" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i0s13i93" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i0s13i91" class="C1_text">ALL
+
-
 
+
-
WRONG</textarea> <br> </td>
+
-
 
+
-
</tr> </tbody></table> </td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s13i10">2013/8/28 12:02:00</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s13i10" class="C1"> <tbody><tr> <td
+
-
 
+
-
class="C1_td"> <img id="i0s13i100" class="C1_graph"
+
-
 
+
-
src="http://trysomething-block.stor.sinaapp.com/t1i0s13i10180 8.28 18o-2m19 连接鉴
+
-
 
+
-
定.jpg"><br> <form id="i0s13i104" target="M7" action="img.php"
+
-
 
+
-
method="post" enctype="multipart/form-data"></form> <input
+
-
 
+
-
name="img" type="file" id="i0s13i102" form="i0s13i104" class="C1_button1"
+
-
 
+
-
required="required" disabled=""> <input name="pname"
+
-
 
+
-
type="hidden" value="t1" form="i0s13i104"> <input name="id"
+
-
 
+
-
type="hidden" value="i0s13i10" form="i0s13i104"> <input
+
-
 
+
-
name="oldgraph" type="hidden" id="i0s13i105" form="i0s13i104" value="t1i0s13i10180 8.28
+
-
 
+
-
18o-2m19 连接鉴定.jpg"> <input id="i0s13i106" type="submit"
+
-
 
+
-
value="submit" form="i0s13i104" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i0s13i103" class="M1_exp_img"></div> </td>
+
-
 
+
-
<td class="C1_td_text"> <textarea id="i0s13i101"
+
-
 
+
-
class="C1_text">18O-AI2M19 ALL WRONG</textarea> <br>
+
-
 
+
-
</td> </tr> </tbody></table> </td> </tr>
+
-
 
+
-
</tbody></table> <div class="M1_exp_stepInfo" title="AI2M19 4
+
-
 
+
-
6 10 14 16 17" id="sb0s14"> <button
+
-
 
+
-
class="M1_exp_step_name_button"><input value="AI2M19 4 6 10 14 16 17"
+
-
 
+
-
class="M1_exp_step_name"></button> <span
+
-
 
+
-
class="M1_exp_step_time">2013/8/29 11:28:51--Ruosang</span> </div>
+
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
-
<td class="M1_exp_item_time" id="ib0s14i0">2013/8/29 11:29:30</td>
+
<td class="M1_exp_item_time" id="ib1s5i0">2013/9/25 20:50:35</td>
-
 
+
-
<td class="M1_exp_item_content"> <table id="i0s14i0"
+
-
 
+
-
class="C9"> <tbody><tr>
+
-
 
+
-
<th colspan="6">Template and Primers</th>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" colspan="3"> choose plasmid </th>
+
-
 
+
-
<th class="C3_td" rowspan="4"> primer1 </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="6" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="AI2M19 (6)  4" class="C3_input"></td> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input value="CAGTGATAGAGATATAATGCACTACTAGAG"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td">
+
-
 
+
-
location </th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> direction </th>
+
-
 
+
-
<td class="C3_td">
+
-
 
+
-
<select id="i0s14i04" class="C3_input">  
+
-
 
+
-
<option value="left">left</option>  
+
-
 
+
-
<option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
position </th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> part-only
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
+
-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
+
-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
+
-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td" rowspan="4"> primer2
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="7" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> length
+
-
 
+
-
</th> <td class="C3_td"><input value="1767"
+
-
 
+
-
class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">sequence</th> <td
+
-
 
+
-
class="C3_td"><input value="ATAGGGATGTCAATCTCTATCACTG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">name</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">direction</th> <td
+
-
 
+
-
class="C3_td"> <select id="i0s14i012"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
class="C3_td">length</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s14i1">2013/8/29 11:30:51</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s14i1" class="C9">
+
-
 
+
-
<tbody><tr> <th colspan="6">Template and
+
-
 
+
-
Primers</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3"> choose plasmid
+
-
 
+
-
</th> <th class="C3_td" rowspan="4"> primer1
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th> <td
+
-
 
+
-
class="C3_td"><input value="8" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="AI2M19 (6)  6"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input value="TGATAGAGATACTGAGCAC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> direction
+
-
 
+
-
</th> <td class="C3_td">
+
-
 
+
-
<select id="i0s14i14" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
position </th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> part-only
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
+
-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
+
-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
+
-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td" rowspan="4"> primer2
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="9" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> length
+
-
 
+
-
</th> <td class="C3_td"><input value="1767"
+
-
 
+
-
class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">sequence</th> <td
+
-
 
+
-
class="C3_td"><input value="TGATAGGGATGTCAATCTC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2"> backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td">name</th> <td class="C3_td"><input
+
-
 
+
-
value="psb1a2" class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">direction</th> <td class="C3_td">
+
-
 
+
-
<select id="i0s14i112" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">length</th>
+
-
 
+
-
<td class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s14i2">2013/8/29 11:32:21</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s14i2" class="C9">
+
-
 
+
-
<tbody><tr> <th colspan="6">Template and
+
-
 
+
-
Primers</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3"> choose plasmid
+
-
 
+
-
</th> <th class="C3_td" rowspan="4"> primer1
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th> <td
+
-
 
+
-
class="C3_td"><input value="0" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="AI2M19 (6)  10"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input value="CGTGATAGAGATACTGAGCAC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
direction </th> <td class="C3_td">
+
-
 
+
-
<select id="i0s14i24" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
position </th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> part-only
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
+
-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
+
-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
+
-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td" rowspan="4"> primer2
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="9" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> length
+
-
 
+
-
</th> <td class="C3_td"><input value="1767"
+
-
 
+
-
class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">sequence</th> <td
+
-
 
+
-
class="C3_td"><input value="TGATAGGGATGTCAATCTC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2"> backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td">name</th> <td class="C3_td"><input
+
-
 
+
-
value="psb1a2" class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">direction</th> <td class="C3_td">
+
-
 
+
-
<select id="i0s14i212" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">length</th>
+
-
 
+
-
<td class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s14i3">2013/8/29 11:34:47</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s14i3" class="C9">
+
-
 
+
-
<tbody><tr> <th colspan="6">Template and
+
-
 
+
-
Primers</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3"> choose plasmid
+
-
 
+
-
</th> <th class="C3_td" rowspan="4"> primer1
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th> <td
+
-
 
+
-
class="C3_td"><input value="PTET14F" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="AI2M19 (6)    14"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input value="GAGAAAGAGGCGAAATAC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> direction
+
-
 
+
-
</th> <td class="C3_td">
+
-
 
+
-
<select id="i0s14i34" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
position </th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> part-only
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
+
-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
+
-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
+
-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td" rowspan="4"> primer2
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="PTET11R" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> length
+
-
 
+
-
</th> <td class="C3_td"><input value="1767"
+
-
 
+
-
class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">sequence</th> <td
+
-
 
+
-
class="C3_td"><input value="TAGTAGTGCTCAGTATCTC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2"> backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td">name</th> <td class="C3_td"><input
+
-
 
+
-
value="psb1a2" class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">direction</th> <td class="C3_td">
+
-
 
+
-
<select id="i0s14i312" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">length</th>
+
-
 
+
-
<td class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s14i4">2013/8/29 11:41:40</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s14i4" class="C9">
+
-
 
+
-
<tbody><tr> <th colspan="6">Template and
+
-
 
+
-
Primers</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3"> choose plasmid
+
-
 
+
-
</th> <th class="C3_td" rowspan="4"> primer1
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th> <td
+
-
 
+
-
class="C3_td"><input value="PTET16F" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="AI2M19 (6)  16"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input value="TACTAGATGCGTAAAGGAG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> direction
+
-
 
+
-
</th> <td class="C3_td">
+
-
 
+
-
<select id="i0s14i44" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
position </th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> part-only
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
+
-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
+
-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
+
-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td" rowspan="4"> primer2
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="PTET16R" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> length
+
-
 
+
-
</th> <td class="C3_td"><input value="1767"
+
-
 
+
-
class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">sequence</th> <td
+
-
 
+
-
class="C3_td"><input value="CTCCTCTTTCTCTAGTAG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2"> backbone
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td">name</th> <td class="C3_td"><input
+
-
 
+
-
value="psb1a2" class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">direction</th> <td class="C3_td">
+
-
 
+
-
<select id="i0s14i412" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">length</th>
+
-
 
+
-
<td class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s14i5">2013/8/29 11:42:42</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s14i5" class="C9">
+
-
 
+
-
<tbody><tr> <th colspan="6">Template and
+
-
 
+
-
Primers</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3"> choose plasmid
+
-
 
+
-
</th> <th class="C3_td" rowspan="4"> primer1
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th> <td
+
-
 
+
-
class="C3_td"><input value="PTET17F" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="AI2M19 (6)  17"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input value="AGGATACTAGATGCGTAAAGGAG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
direction </th> <td class="C3_td">
+
-
 
+
-
<select id="i0s14i54" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
position </th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> part-only
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaat
+
-
 
+
-
cgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
+
-
 
+
-
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
+
-
 
+
-
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcga
+
-
 
+
-
aaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcg
+
-
 
+
-
tattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaa
+
-
 
+
-
ttatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
+
-
 
+
-
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaa
+
-
 
+
-
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
+
-
 
+
-
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatg
+
-
 
+
-
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
+
-
 
+
-
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
+
-
 
+
-
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgta
+
-
 
+
-
caggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcga
+
-
 
+
-
gttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
+
-
 
+
-
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
+
-
 
+
-
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
+
-
 
+
-
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
+
-
 
+
-
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
+
-
 
+
-
ttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td" rowspan="4"> primer2
+
-
 
+
-
</th> <th
+
-
 
+
-
class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="PTET17R" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> length
+
-
 
+
-
</th> <td class="C3_td"><input value="1767"
+
-
 
+
-
class="C3_input"></td> <th
+
-
 
+
-
class="C3_td">sequence</th> <td
+
-
 
+
-
class="C3_td"><input value="TTTCTCCTCTTTCTCTAGTAGTGC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">name</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">direction</th> <td
+
-
 
+
-
class="C3_td"> <select id="i0s14i512"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
class="C3_td">length</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s14i6">2013/8/29 11:43:26</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s14i6" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i0s14i60" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s14i713.8.29 ptet 4 6 10 14 16 17.jpg"><br>
+
-
 
+
-
<form id="i0s14i64" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i0s14i62"
+
-
 
+
-
form="i0s14i64" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i0s14i64">
+
-
 
+
-
<input name="id" type="hidden" value="i0s14i6" form="i0s14i64">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i0s14i65" form="i0s14i64" value="t1i0s14i713.8.29
+
-
 
+
-
ptet 4 6 10 14 16 17.jpg"> <input id="i0s14i66" type="submit"
+
-
 
+
-
value="submit" form="i0s14i64" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i0s14i63" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i0s14i61" class="C1_text">ptet
+
-
 
+
-
4 6 10 14 16 17</textarea> <br> </td>
+
-
 
+
-
</tr> </tbody></table> </td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s14i7">2013/8/29 11:44:18</td> <td
+
-
 
+
-
class="M1_exp_item_content"><textarea id="i0s14i7" class="C0" rows="1">DO THE
+
-
 
+
-
LIGAION</textarea></td> </tr> <tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib0s14i8">2013/8/31 11:44:58</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i0s14i8" class="C1">
+
-
 
+
-
<tbody><tr> <td class="C1_td"> <img
+
-
 
+
-
id="i0s14i80" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s14i92013.08.31CH.jpg"><br> <form
+
-
 
+
-
id="i0s14i84" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i0s14i82"
+
-
 
+
-
form="i0s14i84" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i0s14i84">
+
-
 
+
-
<input name="id" type="hidden" value="i0s14i8" form="i0s14i84">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i0s14i85" form="i0s14i84"
+
-
 
+
-
value="t1i0s14i92013.08.31CH.jpg"> <input id="i0s14i86"
+
-
 
+
-
type="submit" value="submit" form="i0s14i84" class="C1_button2" disabled="">
+
-
 
+
-
<div id="i0s14i83" class="M1_exp_img"></div> </td>
+
-
 
+
-
<td class="C1_td_text"> <textarea id="i0s14i81"
+
-
 
+
-
class="C1_text">4.1  4.2 4.3 9.1 M 9.2 9.3 9.4 9.5 10.1 10.2 10.3 M 14.1 14.2 16.1 16.2 16.3
+
-
 
+
-
17.1 17.2 M 17.2
+
-
C    C    C    C        C    C    C    W  C    C    W      W    C    W 
+
-
 
+
-
W    W  W  W        W
+
-
PBAD LINE0(BL21) LINE1(BL21) LINE2(BL21)
+
-
  C                      C                   
+
-
 
+
-
C</textarea> <br> </td> </tr>
+
-
 
+
-
</tbody></table> </td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib0s14i9">2013/9/2 11:52:55</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i0s14i9" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i0s14i90" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i0s14i1013.9.2 ptet 4.4 10.4 10.5 10.6 14.3 14.4 14.5 16.3 17.1
+
-
 
+
-
17.2.jpg"><br> <form id="i0s14i94" target="M7" action="img.php"
+
-
 
+
-
method="post" enctype="multipart/form-data"></form> <input
+
-
 
+
-
name="img" type="file" id="i0s14i92" form="i0s14i94" class="C1_button1" required="required"
+
-
 
+
-
disabled=""> <input name="pname" type="hidden" value="t1"
+
-
 
+
-
form="i0s14i94"> <input name="id" type="hidden"
+
-
 
+
-
value="i0s14i9" form="i0s14i94"> <input name="oldgraph"
+
-
 
+
-
type="hidden" id="i0s14i95" form="i0s14i94" value="t1i0s14i1013.9.2 ptet 4.4 10.4 10.5 10.6
+
-
 
+
-
14.3 14.4 14.5 16.3 17.1 17.2.jpg"> <input id="i0s14i96"
+
-
 
+
-
type="submit" value="submit" form="i0s14i94" class="C1_button2" disabled="">
+
-
 
+
-
<div id="i0s14i93" class="M1_exp_img"></div> </td>
+
-
 
+
-
<td class="C1_td_text"> <textarea id="i0s14i91"
+
-
 
+
-
class="C1_text">4.4 10.4 10.5 10.6 14.3 M 14.4 14.5 16.3 17.1 17.2
+
-
ALL WRONG</textarea> <br>
+
-
 
+
-
</td> </tr> </tbody></table> </td> </tr>
+
-
 
+
-
</tbody></table>
+
-
</div>
+
-
</div>
+
-
 
+
-
<div id="t1e2">
+
-
<div class="dataOutput_head">
+
-
pBAD-2M19
+
-
</div>
+
-
<div class="dataOutput2">
+
-
<div class="M1_exp_stepInfo" title="pBAD" id="sb1s0">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="pBAD"
+
-
 
+
-
class="M1_exp_step_name"></button> <span
+
-
 
+
-
class="M1_exp_step_time">2013/8/3 18:01:27--Ruosang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s0i0">2013/8/3 18:01:51</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s0i0" class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="2"
+
-
 
+
-
class="C8_td_title"> Start to Ligate Plasmids
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="pbad" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="pbad"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="130" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2155" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (B)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location </th>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td"> type
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> part-only
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> backbone
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
</tbody></table> </td>
+
-
 
+
-
<td style="display: none;"> <table class="C8">
+
-
 
+
-
<tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> plasmid
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location </th>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td"> type
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> part-only
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> backbone
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
</tbody></table> </td>
+
-
 
+
-
</tr> <tr> <td class="C3_td"
+
-
 
+
-
colspan="2"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i1s0i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i1s0i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s0i1">2013/8/3 18:02:03</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s0i1" class="C4">
+
-
 
+
-
<tbody><tr> <th colspan="3">Plasmid
+
-
 
+
-
Extraction</th> <td rowspan="9"
+
-
 
+
-
class="C4_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s0i19">
+
-
 
+
-
</textarea></td> </tr>
+
-
 
+
-
<tr> <td class="C3_td">      </td>
+
-
 
+
-
<th class="C3_td">A<sub>260/280</sub></th>
+
-
 
+
-
<th class="C3_td">ng/μL</th>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" align="center">(A)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.88" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td" align="center">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C4_tr_C" style="display: none;">
+
-
 
+
-
<td class="C3_td" align="center">(C)</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3">
+
-
 
+
-
<button class="C8_button" id="i1s0i112" name="#i1s0i1">
+
-
 
+
-
Evaluate
+
-
 
+
-
</button> </th>
+
-
 
+
-
</tr> <tr style="display: none;">
+
-
 
+
-
<td colspan="3">
+
-
 
+
-
<table class="C3"> <tbody><tr>
+
-
 
+
-
<td
+
-
 
+
-
class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td>
+
-
 
+
-
<td class="C3_td">great</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td">fine</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td>
+
-
 
+
-
<td class="C3_td" rowspan="2">not good</td>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
</tbody></table></td> </tr> <tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s0i2">2013/8/3 18:03:36</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table class="C5" id="i1s0i2">
+
-
 
+
-
<tbody><tr> <th colspan="3" id="i1s0i229"
+
-
 
+
-
class="C5_th_C">Extract plasmid</th> <td rowspan="10"
+
-
 
+
-
class="C3_td_textarea"> <textarea class="C3_textarea"
+
-
 
+
-
id="i1s0i20"> </textarea>
+
-
 
+
-
</td> </tr> <tr> <th
+
-
 
+
-
class="C5_canvas_td" colspan="3" id="i1s0i230"> <div
+
-
 
+
-
class="C5_canvas_matrix"> </div>
+
-
 
+
-
<div class="C5_TEXT1">EX</div> <div
+
-
 
+
-
class="C5_TEXT2">SP</div> <input type="button"
+
-
 
+
-
class="C5_canvas_button1" id="i1s0i21" name="#i1s0i2" value="pbad (A)">
+
-
 
+
-
<input type="button" class="C5_canvas_button2" id="i1s0i22" name="#i1s0i2" value="
+
-
 
+
-
(B)"> <input type="button" class="C5_canvas_button3"
+
-
 
+
-
id="i1s0i23" name="#i1s0i2" value="pbad (A)"> </th>
+
-
 
+
-
</tr> <tr> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="100" class="C5_system_input"></th>
+
-
 
+
-
<th class="C3_td">(A)</th> <th class="C3_td">
+
-
 
+
-
(B)</th> <th class="C3_td_C5_C" style="display:
+
-
 
+
-
none;">(C)</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Plasmid</th> <td
+
-
 
+
-
class="C3_td"><input value="40" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="0" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="10"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Eco</em>R I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Xba</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Spe</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Pst</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">H<sub>2</sub>0 /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="40" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="80" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="18"
+
-
 
+
-
class="C3_input"></td> <input type="hidden" id="i1s0i226"
+
-
 
+
-
value="0"> <input type="hidden" id="i1s0i227"
+
-
 
+
-
value="1"> <input type="hidden" id="i1s0i228"
+
-
 
+
-
value="0"> </tr> </tbody></table></td>
+
-
 
+
-
</tr> <tr class="M1_exp_stepItem"> <td
+
-
 
+
-
class="M1_exp_item_time" id="ib1s0i3">2013/8/3 18:03:49</td> <td
+
-
class="M1_exp_item_content"> <table id="i1s0i3" class="C1"> <tbody><tr>
+
<td class="M1_exp_item_content"> <table id="i1s5i0" class="C1"> <tbody><tr>
-
<td class="C1_td"> <img id="i1s0i30" class="C1_graph"  
+
<td class="C1_td"> <img id="i1s5i00"  
-
src="http://trysomething-block.stor.sinaapp.com/t1i1s0i313.8.3 牛津样，pBAD.jpg"><br>
+
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s5i0915.gif"><br>
-
<form id="i1s0i34" target="M7" action="img.php" method="post"  
+
<form id="i1s5i04" target="M7" action="img.php" method="post"  
enctype="multipart/form-data"></form> <input name="img"  
enctype="multipart/form-data"></form> <input name="img"  
-
type="file" id="i1s0i32" form="i1s0i34" class="C1_button1" required="required" disabled="">
+
type="file" id="i1s5i02" form="i1s5i04" class="C1_button1" required="required" disabled="">
-
<input name="pname" type="hidden" value="t1" form="i1s0i34">
+
<input name="pname" type="hidden" value="t14" form="i1s5i04">
-
<input name="id" type="hidden" value="i1s0i3" form="i1s0i34">
+
<input name="id" type="hidden" value="i1s5i0" form="i1s5i04">
-
<input name="oldgraph" type="hidden" id="i1s0i35" form="i1s0i34"  
+
<input name="oldgraph" type="hidden" id="i1s5i05" form="i1s5i04"  
-
value="t1i1s0i313.8.3 牛津样，pBAD.jpg"> <input id="i1s0i36"  
+
value="t14i1s5i0915.gif"> <input id="i1s5i06" type="submit"  
-
type="submit" value="submit" form="i1s0i34" class="C1_button2" disabled="">
+
value="submit" form="i1s5i04" class="C1_button2" disabled=""> <div
-
<div id="i1s0i33" class="M1_exp_img"></div> </td>
+
id="i1s5i03" class="M1_exp_img"></div> </td> <td
-
<td class="C1_td_text"> <textarea id="i1s0i31"  
+
class="C1_td_text"> <textarea id="i1s5i01" class="C1_text">15th
-
class="C1_text">OTHER SAMPLE M(500) PBAD
+
September
 +
Plasmid:Gfp(pSB1C3) aiia(pSB3T5)
 +
Bacteria: BL21(WT)
 +
Condition: Flowing medium culture supply. Cells could flow into different traps. With
-
WRONG AND DO THIS AGAIN TOMORROW</textarea> <br>
+
antibiotic putting in.
 +
Total Obserbation Time:515mins
 +
Analysis:
 +
From all the mircrofluid images, there is a tendency that the fluorescence degree change
-
</td> </tr> </tbody></table> </td> </tr>
+
from weak to strong and then change from strong to weak.
 +
</textarea> <br> </td> </tr>
-
</tbody></table> <div class="M1_exp_stepInfo" title="PBAD-
+
</tbody></table> </td> </tr> </tbody></table>
-
2M19" id="sb1s1"> <button class="M1_exp_step_name_button"><input
+
<div class="M1_exp_stepInfo" title=" 0918 BL21(WT) sfGfp(coE1) aiia(pSB3T5)"  
-
value="PBAD-2M19" class="M1_exp_step_name"></button> <span
+
id="sb1s6"> <button class="M1_exp_step_name_button"><input value=" 0918
-
class="M1_exp_step_time">2013/8/3 18:04:55--Ruosang</span> </div>
+
BL21(WT) sfGfp(coE1) aiia(pSB3T5)" class="M1_exp_step_name"></button>
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
<span class="M1_exp_step_time">2013/9/18 10:53:24--Chun Tang</span> </div>
-
 
+
-
<td class="M1_exp_item_time" id="ib1s1i0">2013/8/3 18:05:49</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s1i0" class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="2"
+
-
 
+
-
class="C8_td_title"> Start to Ligate Plasmids
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="pbad 1 (5)" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="pbad"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="130" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2155" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (B)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="pbad 2 (5)" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="pbad"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="130" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2155" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td style="display: none;">
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="2"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i1s1i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i1s1i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s1i1">2013/8/3 18:08:04</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s1i1" class="C4">
+
-
 
+
-
<tbody><tr> <th colspan="3">Plasmid
+
-
 
+
-
Extraction</th> <td rowspan="9"
+
-
 
+
-
class="C4_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s1i19">
+
-
 
+
-
</textarea></td> </tr>
+
-
 
+
-
<tr> <td class="C3_td">      </td>
+
-
 
+
-
<th class="C3_td">A<sub>260/280</sub></th>
+
-
 
+
-
<th class="C3_td">ng/μL</th>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" align="center">(A)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.85" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="105.0" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td" align="center">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.86" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="148.6" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C4_tr_C" style="display: none;">
+
-
 
+
-
<td class="C3_td" align="center">(C)</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" colspan="3">
+
-
 
+
-
<button class="C8_button" id="i1s1i112" name="#i1s1i1">
+
-
 
+
-
Evaluate
+
-
 
+
-
</button> </th>
+
-
 
+
-
</tr> <tr style="display: none;">
+
-
 
+
-
<td colspan="3">
+
-
 
+
-
<table class="C3">
+
-
 
+
-
<tbody><tr> <td
+
-
 
+
-
class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td>
+
-
 
+
-
<td class="C3_td">great</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td">fine</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td>
+
-
 
+
-
<td class="C3_td" rowspan="2">not good</td>
+
-
 
+
-
<td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
</tbody></table></td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="PBAD-2M19 PBAD IS THE BACKBONE" id="sb1s2">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="PBAD-2M19 PBAD IS THE BACKBONE"
+
-
 
+
-
class="M1_exp_step_name"></button> <span  
+
-
 
+
-
class="M1_exp_step_time">2013/8/3 18:10:12--Ruosang</span> </div>
+
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
-
<td class="M1_exp_item_time" id="ib1s2i0">2013/8/3 18:10:41</td>
+
<td class="M1_exp_item_time" id="ib1s6i0">2013/9/25 20:52:02</td>
-
<td class="M1_exp_item_content"> <table id="i1s2i0" class="C8">
+
<td class="M1_exp_item_content"> <table id="i1s6i0" class="C1"> <tbody><tr>
-
<tbody><tr> <th colspan="2"  
+
<td class="C1_td"> <img id="i1s6i00"  
-
class="C8_td_title"> Start to Ligate Plasmids
+
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s6i0918.gif"><br>
-
</th> </tr>
+
<form id="i1s6i04" target="M7" action="img.php" method="post"  
-
 
+
-
<tr> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="pbad" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="pbad"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="130" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2155" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (B)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2M19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="rbs-gfp-tt"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctg
+
-
 
+
-
tcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaaca
+
-
 
+
-
cttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccga
+
-
 
+
-
aggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtta
+
-
 
+
-
atagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatac
+
-
 
+
-
atcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccatta
+
-
 
+
-
tcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacg
+
-
 
+
-
aaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagc
+
-
 
+
-
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
+
-
 
+
-
ctggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="857" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td style="display: none;">
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="2"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i1s2i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i1s2i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s2i1">2013/8/3 18:11:50</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table class="C5" id="i1s2i1"> <tbody><tr>
+
-
 
+
-
<th colspan="3" id="i1s2i129" class="C5_th_C">Extract plasmid</th>
+
-
 
+
-
<td rowspan="10" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s2i10"> </textarea>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C5_canvas_td" colspan="3" id="i1s2i130"> <div
+
-
 
+
-
class="C5_canvas_matrix"> </div>
+
-
 
+
-
<div class="C5_TEXT1">EX</div> <div
+
-
 
+
-
class="C5_TEXT2">SP</div> <input type="button"
+
-
 
+
-
class="C5_canvas_button1" id="i1s2i11" name="#i1s2i1" value="pbad (A)">
+
-
 
+
-
<input type="button" class="C5_canvas_button2" id="i1s2i12" name="#i1s2i1"
+
-
 
+
-
value="2M19 (B)"> <input type="button"
+
-
 
+
-
class="C5_canvas_button3" id="i1s2i13" name="#i1s2i1" value="pbad (A)">
+
-
 
+
-
</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th>
+
-
 
+
-
<th class="C3_td">(A)</th> <th
+
-
 
+
-
class="C3_td">(B)</th> <th class="C3_td_C5_C"
+
-
 
+
-
style="display: none;">(C)</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Plasmid</th>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="62" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="10"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Eco</em>R I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Xba</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Spe</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Pst</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">H<sub>2</sub>0 /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="60" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="18" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="18"
+
-
 
+
-
class="C3_input"></td> <input type="hidden" id="i1s2i126"
+
-
 
+
-
value="0"> <input type="hidden" id="i1s2i127"
+
-
 
+
-
value="1"> <input type="hidden" id="i1s2i128"
+
-
 
+
-
value="0"> </tr> </tbody></table></td>
+
-
 
+
-
</tr> <tr class="M1_exp_stepItem"> <td
+
-
 
+
-
class="M1_exp_item_time" id="ib1s2i2">2013/8/3 19:45:37</td> <td
+
-
 
+
-
class="M1_exp_item_content"> <table id="i1s2i2" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s2i20" class="C1_graph"
+
-
 
+
-
src="http://trysomething-block.stor.sinaapp.com/t1i1s2i913.8.4 牛津样，pBAD.jpg"><br>
+
-
 
+
-
<form id="i1s2i24" target="M7" action="img.php" method="post"  
+
enctype="multipart/form-data"></form> <input name="img"  
enctype="multipart/form-data"></form> <input name="img"  
-
type="file" id="i1s2i22" form="i1s2i24" class="C1_button1" required="required" disabled="">
+
type="file" id="i1s6i02" form="i1s6i04" class="C1_button1" required="required" disabled="">
-
<input name="pname" type="hidden" value="t1" form="i1s2i24">
+
<input name="pname" type="hidden" value="t14" form="i1s6i04">
-
<input name="id" type="hidden" value="i1s2i2" form="i1s2i24">
+
<input name="id" type="hidden" value="i1s6i0" form="i1s6i04">
-
<input name="oldgraph" type="hidden" id="i1s2i25" form="i1s2i24"  
+
<input name="oldgraph" type="hidden" id="i1s6i05" form="i1s6i04"  
-
value="t1i1s2i913.8.4 牛津样，pBAD.jpg"> <input id="i1s2i26"  
+
value="t14i1s6i0918.gif"> <input id="i1s6i06" type="submit"  
-
type="submit" value="submit" form="i1s2i24" class="C1_button2" disabled="">
+
value="submit" form="i1s6i04" class="C1_button2" disabled=""> <div
-
<div id="i1s2i23" class="M1_exp_img"></div> </td>
+
id="i1s6i03" class="M1_exp_img"></div> </td> <td
-
<td class="C1_td_text"> <textarea id="i1s2i21"  
+
class="C1_td_text"> <textarea id="i1s6i01" class="C1_text">18th
-
class="C1_text">OTHER SAMPLE M(500) PBAD</textarea> <br>
+
September
 +
Plasmid:sfGfp(coE1) aiia(pSB3T5)
 +
Bacteria: BL21(WT)
 +
Condition: Flowing medium culture supply. Cells could flow into different traps. With
-
</td> </tr> </tbody></table> </td> </tr>
+
antibiotic putting in.
 +
Total Obserbation Time:590mins
 +
Analysis:
 +
There was a strong tendency that the fluorescence intensity came down, and it’s the first
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
time that we detected the most obvious changing.
 +
</textarea> <br> </td> </tr>
-
id="ib1s2i3">2013/8/3 18:13:13</td> <td class="M1_exp_item_content">
+
</tbody></table> </td> </tr> </tbody></table>
-
<table id="i1s2i3" class="C6"> <tbody><tr>
+
<div class="M1_exp_stepInfo" title="0920 BL21(WT) sfGfp(coE1) aiia(pSB3T5)"  
-
<th class="C3_td_title" colspan="5">Electrophoretogram and
+
id="sb1s7"> <button class="M1_exp_step_name_button"><input value="0920
-
recover</th> <td rowspan="10" class="C6_td_textarea">
+
BL21(WT) sfGfp(coE1) aiia(pSB3T5)" class="M1_exp_step_name"></button>
-
<textarea class="C3_textarea" id="i1s2i36">
+
<span class="M1_exp_step_time">2013/9/25 21:10:34--Chun Tang</span> </div>
-
 
+
-
</textarea> </td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
colspan="5" class="C3_td"> Analyse
+
-
 
+
-
Electrophoretogram </th>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct1s2i3">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">A<sub>260/280</sub></th> <th
+
-
 
+
-
class="C3_td">ng/μL</th> <th class="C3_td">bp</th>
+
-
 
+
-
<td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Correct" class="C6_button" name="1s2i3">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Correct1s2i3"> <td class="C3_td"
+
-
 
+
-
nowrap="nowrap">(A)</td> <td class="C3_td"><input
+
-
 
+
-
value="1.79" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="13.8" class="C6_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="2285" class="C6_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct1s2i3">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.86" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="2.6" class="C6_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="857" class="C6_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct1s2i3" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap">(C)</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Wrong1s2i3" style="display: none;">
+
-
 
+
-
<td class="C3_td" colspan="4" rowspan="3">
+
-
 
+
-
Possible reason:<br> 1. The
+
-
 
+
-
validity or contamination of gel extraction kit and DD water.<br>
+
-
 
+
-
2. The validity of enzyme and buffer<br>
+
-
 
+
-
3. Incomplete dissolution of agar gel<br>
+
-
 
+
-
</td> <td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Wrong" class="C6_button" name="1s2i3">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Wrong1s2i3" style="display: none;"> </tr>
+
-
 
+
-
<tr class="mdoelC5Wrong1s2i3" style="display: none;">
+
-
 
+
-
</tr> <tr class="mdoelC5Wrong1s2i3" style="display: none;">
+
-
 
+
-
</tr> </tbody></table> <input
+
-
 
+
-
type="hidden" id="i1s2i37" value="1"></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s2i4">2013/8/3 18:13:35</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s2i4" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s2i40"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i1s2i426">A
+
-
 
+
-
(vector) /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="1.1474428306551299" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
id="i1s2i427">B (insert) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="6.85255716934487" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<th class="C3_td">ligase /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:  
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s2i5">2013/8/3 18:14:18</td> <td
+
-
 
+
-
class="M1_exp_item_content"><textarea id="i1s2i5" class="C0"
+
-
 
+
-
rows="1">TRANSFORMATION</textarea></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s2i6">2013/8/5 11:54:23</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s2i6" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s2i60"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i1s2i626">A
+
-
 
+
-
(vector) /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="1.1474428306551299" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
id="i1s2i627">B (insert) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="6.85255716934487" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="7" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<th class="C3_td">ligase /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s2i7">2013/8/8 12:43:18</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s2i7" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s2i70" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s2i713.8.8(M 1A 2A M 3A 1B M 2B 3B M).jpg"><br>
+
-
 
+
-
<form id="i1s2i74" target="M7" action="img.php" method="post"
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"
+
-
 
+
-
type="file" id="i1s2i72" form="i1s2i74" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s2i74">
+
-
 
+
-
<input name="id" type="hidden" value="i1s2i7" form="i1s2i74">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s2i75" form="i1s2i74"
+
-
 
+
-
value="t1i1s2i713.8.8(M 1A 2A M 3A 1B M 2B 3B M).jpg"> <input
+
-
 
+
-
id="i1s2i76" type="submit" value="submit" form="i1s2i74" class="C1_button2" disabled="">
+
-
 
+
-
<div id="i1s2i73" class="M1_exp_img"></div>
+
-
 
+
-
</td> <td class="C1_td_text"> <textarea
+
-
 
+
-
id="i1s2i71" class="C1_text">ANALYSE BY PCR
+
-
1819(23) pbad2m19 1819(34)1 1819(34)2 1819(35)1 1819(35)2
+
-
C              W            C                  C              C           
+
-
 
+
-
C            </textarea> <br> </td>
+
-
 
+
-
</tr> </tbody></table> </td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s2i8">2013/8/9 12:49:26</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s2i8" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s2i80" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s2i813.8.9 PBAD 123456鉴定.jpg"><br>
+
-
 
+
-
<form id="i1s2i84" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i1s2i82"
+
-
 
+
-
form="i1s2i84" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s2i84">
+
-
 
+
-
<input name="id" type="hidden" value="i1s2i8" form="i1s2i84">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s2i85" form="i1s2i84" value="t1i1s2i813.8.9 PBAD
+
-
 
+
-
123456鉴定.jpg"> <input id="i1s2i86" type="submit"
+
-
 
+
-
value="submit" form="i1s2i84" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i1s2i83" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s2i81"
+
-
 
+
-
class="C1_text">ANALYSE BY PCR
+
-
PBAD 1  2  3  4  5  6
+
-
  C  C  C</textarea>
+
-
 
+
-
<br> </td> </tr> </tbody></table> </td>
+
-
 
+
-
</tr> </tbody></table> <div class="M1_exp_stepInfo"
+
-
 
+
-
title="PBAD-2M19 2M19 IS THE BACKBONE" id="sb1s3"> <button
+
-
 
+
-
class="M1_exp_step_name_button"><input value="PBAD-2M19 2M19 IS THE BACKBONE"
+
-
 
+
-
class="M1_exp_step_name"></button> <span
+
-
 
+
-
class="M1_exp_step_time">2013/8/10 22:17:02--Ruosang</span> </div>
+
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
-
<td class="M1_exp_item_time" id="ib1s3i0">2013/8/10 22:17:31</td>
+
<td class="M1_exp_item_time" id="ib1s7i0">2013/9/25 21:11:06</td>
-
<td class="M1_exp_item_content"> <table id="i1s3i0" class="C8">
+
<td class="M1_exp_item_content"> <table id="i1s7i0" class="C1"> <tbody><tr>
-
<tbody><tr> <th colspan="2"  
+
<td class="C1_td"> <img id="i1s7i00"  
-
class="C8_td_title"> Start to Ligate Plasmids
+
class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t14i1s8i0920.gif"><br>
-
</th> </tr>
+
<form id="i1s7i04" target="M7" action="img.php" method="post"  
-
 
+
-
<tr> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="pbad" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="pbad"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="130" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2155" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (B)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2M19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="rbs-gfp-tt"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctg
+
-
 
+
-
tcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaaca
+
-
 
+
-
cttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccga
+
-
 
+
-
aggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtta
+
-
 
+
-
atagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatac
+
-
 
+
-
atcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccatta
+
-
 
+
-
tcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacg
+
-
 
+
-
aaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagc
+
-
 
+
-
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
+
-
 
+
-
ctggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="857" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="2079" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table>
+
-
 
+
-
</td> <td style="display: none;">
+
-
 
+
-
<table class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="3"
+
-
 
+
-
class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
plasmid
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<td class="C3_td" colspan="2"> <input
+
-
 
+
-
type="button" class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i1s3i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i1s3i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s3i1">2013/8/10 22:17:54</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table class="C5" id="i1s3i1"> <tbody><tr>
+
-
 
+
-
<th colspan="3" id="i1s3i129" class="C5_th_C">Extract plasmid</th>
+
-
 
+
-
<td rowspan="10" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s3i10"> </textarea>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C5_canvas_td" colspan="3" id="i1s3i130"> <div
+
-
 
+
-
class="C5_canvas_matrix"> </div>
+
-
 
+
-
<div class="C5_TEXT1">EX</div> <div
+
-
 
+
-
class="C5_TEXT2">SP</div> <input type="button"
+
-
 
+
-
class="C5_canvas_button1" id="i1s3i11" name="#i1s3i1" value="pbad (A)">
+
-
 
+
-
<input type="button" class="C5_canvas_button2" id="i1s3i12" name="#i1s3i1"
+
-
 
+
-
value="2M19 (B)"> <input type="button"
+
-
 
+
-
class="C5_canvas_button3" id="i1s3i13" name="#i1s3i1" value="2M19 (B)">
+
-
 
+
-
</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th>
+
-
 
+
-
<th class="C3_td">(A)</th> <th
+
-
 
+
-
class="C3_td">(B)</th> <th class="C3_td_C5_C"
+
-
 
+
-
style="display: none;">(C)</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Plasmid</th>
+
-
 
+
-
<td class="C3_td"><input value="60" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="50" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="10" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="10"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Eco</em>R I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Xba</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Spe</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="5" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="0"
+
-
 
+
-
class="C3_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td"><em>Pst</em> I /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0" class="C5_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="5"
+
-
 
+
-
class="C5_input"></td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">H<sub>2</sub>0 /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="30" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td_C5_C" style="display: none;"><input value="18"
+
-
 
+
-
class="C3_input"></td> <input type="hidden" id="i1s3i126"
+
-
 
+
-
value="0"> <input type="hidden" id="i1s3i127"
+
-
 
+
-
value="1"> <input type="hidden" id="i1s3i128"
+
-
 
+
-
value="1"> </tr> </tbody></table></td>
+
-
 
+
-
</tr> <tr class="M1_exp_stepItem"> <td
+
-
 
+
-
class="M1_exp_item_time" id="ib1s3i2">2013/8/10 22:18:10</td> <td
+
-
 
+
-
class="M1_exp_item_content"> <table id="i1s3i2" class="C1"> <tbody><tr>
+
-
 
+
-
<td class="C1_td"> <img id="i1s3i20" class="C1_graph"
+
-
 
+
-
src="http://trysomething-block.stor.sinaapp.com/t1i1s3i213.8.10 回收pbad 2m19.jpg"><br>
+
-
 
+
-
<form id="i1s3i24" target="M7" action="img.php" method="post"  
+
enctype="multipart/form-data"></form> <input name="img"  
enctype="multipart/form-data"></form> <input name="img"  
-
type="file" id="i1s3i22" form="i1s3i24" class="C1_button1" required="required" disabled="">
+
type="file" id="i1s7i02" form="i1s7i04" class="C1_button1" required="required" disabled="">
-
<input name="pname" type="hidden" value="t1" form="i1s3i24">
+
<input name="pname" type="hidden" value="t14" form="i1s7i04">
-
<input name="id" type="hidden" value="i1s3i2" form="i1s3i24">
+
<input name="id" type="hidden" value="i1s7i0" form="i1s7i04">
-
<input name="oldgraph" type="hidden" id="i1s3i25" form="i1s3i24"  
+
<input name="oldgraph" type="hidden" id="i1s7i05" form="i1s7i04"  
-
value="t1i1s3i213.8.10 回收pbad 2m19.jpg"> <input id="i1s3i26"  
+
value="t14i1s8i0920.gif"> <input id="i1s7i06" type="submit"  
-
type="submit" value="submit" form="i1s3i24" class="C1_button2" disabled="">
+
value="submit" form="i1s7i04" class="C1_button2" disabled=""> <div
-
<div id="i1s3i23" class="M1_exp_img"></div> </td>
+
id="i1s7i03" class="M1_exp_img"></div> </td> <td
-
<td class="C1_td_text"> <textarea id="i1s3i21"  
+
class="C1_td_text"> <textarea id="i1s7i01" class="C1_text">20th
-
class="C1_text">PBAD M1000 2M19</textarea> <br>
+
September
 +
Plasmid:sfGfp(coE1) aiia(pSB3T5)
 +
Bacteria: BL21(WT)
 +
Condition: Flowing medium culture supply. Cells could flow into different traps. With
-
</td> </tr> </tbody></table> </td> </tr>
+
antibiotic putting in.
 +
Total Obserbation Time:470mins
 +
Analysis:
 +
From all the microfluid images, there is a very weak decrease of the fluorescence degree.
 +
</textarea> <br> </td> </tr>
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
</tbody></table> </td> </tr> </tbody></table>
-
id="ib1s3i3">2013/8/10 22:18:46</td> <td class="M1_exp_item_content">
+
<div class="M1_exp_stepInfo" title="0922 BL21(WT) sfGfp(coE1) aiia(pSB3T5)"  
-
<table id="i1s3i3" class="C6"> <tbody><tr>
+
id="sb1s8"> <button class="M1_exp_step_name_button"><input value="0922
-
<th class="C3_td_title" colspan="5">Electrophoretogram and
+
BL21(WT) sfGfp(coE1) aiia(pSB3T5)" class="M1_exp_step_name"></button>
-
 
+
-
recover</th> <td rowspan="10" class="C6_td_textarea">
+
-
 
+
-
<textarea class="C3_textarea" id="i1s3i36">
+
-
 
+
-
</textarea> </td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
colspan="5" class="C3_td"> Analyse
+
-
 
+
-
Electrophoretogram </th>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct1s3i3">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">A<sub>260/280</sub></th> <th
+
-
 
+
-
class="C3_td">ng/μL</th> <th class="C3_td">bp</th>
+
-
 
+
-
<td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Correct" class="C6_button" name="1s3i3">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Correct1s3i3"> <td class="C3_td"
+
-
 
+
-
nowrap="nowrap">(A)</td> <td class="C3_td"><input
+
-
 
+
-
value="1.41" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="9.7" class="C6_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="130" class="C6_input"></td> </tr>
+
-
 
+
-
<tr class="mdoelC5Correct1s3i3"> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="1.41" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="5.9" class="C6_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="2936" class="C6_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct1s3i3" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap">(C)</td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Wrong1s3i3" style="display: none;">
+
-
 
+
-
<td class="C3_td" colspan="4" rowspan="3">
+
-
 
+
-
Possible reason:<br> 1. The
+
-
 
+
-
validity or contamination of gel extraction kit and DD water.<br>
+
-
 
+
-
2. The validity of enzyme and buffer<br>
+
-
 
+
-
3. Incomplete dissolution of agar gel<br>
+
-
 
+
-
</td> <td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Wrong" class="C6_button" name="1s3i3">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Wrong1s3i3" style="display: none;"> </tr>
+
-
 
+
-
<tr class="mdoelC5Wrong1s3i3" style="display: none;">
+
-
 
+
-
</tr> <tr class="mdoelC5Wrong1s3i3" style="display: none;">
+
-
 
+
-
</tr> </tbody></table> <input
+
-
 
+
-
type="hidden" id="i1s3i37" value="1"></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s3i4">2013/8/10 22:19:07</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s3i4" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s3i40"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i1s3i426">A
+
-
 
+
-
(insert) /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="0.5980467963171129" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="0.75" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" id="i1s3i427">B (vector) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="7.401953203682887" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="7.5" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<th class="C3_td">ligase /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s3i5">2013/8/10 22:19:36</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s3i5" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s3i50"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i1s3i526">A
+
-
 
+
-
(insert) /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="0.5980467963171129" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="7.5" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
id="i1s3i527">B (vector) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="7.401953203682887" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="0.75" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<th class="C3_td">ligase /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s3i6">2013/8/10 22:20:43</td> <td
+
-
 
+
-
class="M1_exp_item_content"><textarea id="i1s3i6" class="C0"
+
-
 
+
-
rows="1">TRANSFORMATION</textarea></td> </tr> </tbody></table>
+
-
 
+
-
<div class="M1_exp_stepInfo" title="PBAD2M19  4" id="sb1s4">
+
-
 
+
-
<button class="M1_exp_step_name_button"><input value="PBAD2M19  4"
+
-
 
+
-
class="M1_exp_step_name"></button> <span
+
-
class="M1_exp_step_time">2013/8/13 23:24:12--Ruosang</span> </div>
+
<span class="M1_exp_step_time">2013/9/25 21:12:48--Chun Tang</span> </div>
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
-
<td class="M1_exp_item_time" id="ib1s4i0">2013/8/13 23:25:39</td>
+
<td class="M1_exp_item_time" id="ib1s8i0">2013/9/25 21:13:01</td>
-
<td class="M1_exp_item_content"> <table id="i1s4i0"  
+
<td class="M1_exp_item_content"> <table id="i1s8i0" class="C1"> <tbody><tr>
-
class="C9"> <tbody><tr>
+
<td class="C1_td"> <img id="i1s8i00"
-
<th colspan="6">Template and Primers</th>
+
class="C1_graph" src="http://1.trysomething.sinaapp.com/M.php"><br>
-
</tr> <tr> <th
+
<form id="i1s8i04" target="M7" action="img.php" method="post" enctype="multipart/form-
-
class="C3_td" colspan="3"> choose plasmid </th>
+
data"></form> <input name="img" type="file" id="i1s8i02"  
-
<th class="C3_td" rowspan="4"> primer1 </th>
+
form="i1s8i04" class="C1_button1" required="required" disabled="">
-
<th class="C3_td"> name </th>
+
<input name="pname" type="hidden" value="t14" form="i1s8i04">
-
<td class="C3_td"><input value="P4" class="C3_input"></td>
+
<input name="id" type="hidden" value="i1s8i0" form="i1s8i04">
-
</tr> <tr>
+
<input name="oldgraph" type="hidden" id="i1s8i05" form="i1s8i04" value="">
-
<th class="C3_td" rowspan="2"> plasmid </th>
+
<input id="i1s8i06" type="submit" value="submit" form="i1s8i04" class="C1_button2"  
-
<th class="C3_td"> name
+
disabled=""> <div id="i1s8i03" class="M1_exp_img"></div>
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="pBAD2M19 (7)" class="C3_input"></td> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input value="CAAAGTGTGACGCCGTGC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location
+
-
 
+
-
</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
direction </th> <td
+
-
 
+
-
class="C3_td"> <select id="i1s4i04"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th class="C3_td"
+
-
 
+
-
colspan="2"> type </th> <td
+
-
 
+
-
class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> position
+
-
 
+
-
</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" rowspan="2">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
+
-
 
+
-
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
+
-
 
+
-
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
+
-
 
+
-
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
+
-
 
+
-
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
+
-
 
+
-
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
+
-
 
+
-
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaa
+
-
 
+
-
ccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
+
-
 
+
-
ttacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
+
-
 
+
-
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td"
+
-
 
+
-
rowspan="4"> primer2
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value="P5" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td"> length </th>
+
-
 
+
-
<td class="C3_td"><input value="136" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">sequence</th>
+
-
 
+
-
<td class="C3_td"><input value="GCCATAGCAAGATAGTCC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">name</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">direction</th> <td
+
-
 
+
-
class="C3_td"> <select id="i1s4i012"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
class="C3_td">length</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s4i1">2013/8/13 23:28:09</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s4i1" class="C9">
+
-
 
+
-
<tbody><tr> <th colspan="6">Template and
+
-
 
+
-
Primers</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3"> choose plasmid
+
-
 
+
-
</th> <th class="C3_td" rowspan="4"> primer1
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th> <td
+
-
 
+
-
class="C3_td"><input value="P6" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="pBAD2M19 (7)"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input value="AGCAAAGTGTGACGCCGTG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> direction
+
-
 
+
-
</th> <td class="C3_td">
+
-
 
+
-
<select id="i1s4i14" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> position
+
-
 
+
-
</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" rowspan="2">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
+
-
 
+
-
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
+
-
 
+
-
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
+
-
 
+
-
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
+
-
 
+
-
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
+
-
 
+
-
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
+
-
 
+
-
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaa
+
-
 
+
-
ccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
+
-
 
+
-
ttacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
+
-
 
+
-
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td"
+
-
 
+
-
rowspan="4"> primer2
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value="P5" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td"> length </th>
+
-
 
+
-
<td class="C3_td"><input value="136" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">sequence</th>
+
-
 
+
-
<td class="C3_td"><input value="GCCATAGCAAGATAGTCC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">name</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">direction</th> <td
+
-
 
+
-
class="C3_td"> <select id="i1s4i112"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
class="C3_td">length</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s4i2">2013/8/13 23:28:51</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s4i2" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s4i20" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s4i213.8.13 pbad2m19(45)(67)  19ai2m19 18ai.jpg"><br>
+
-
 
+
-
<form id="i1s4i24" target="M7" action="img.php" method="post"
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"
+
-
 
+
-
type="file" id="i1s4i22" form="i1s4i24" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s4i24">
+
-
 
+
-
<input name="id" type="hidden" value="i1s4i2" form="i1s4i24">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s4i25" form="i1s4i24"
+
-
 
+
-
value="t1i1s4i213.8.13 pbad2m19(45)(67)  19ai2m19 18ai.jpg">
+
-
 
+
-
<input id="i1s4i26" type="submit" value="submit" form="i1s4i24" class="C1_button2"
+
-
 
+
-
disabled=""> <div id="i1s4i23" class="M1_exp_img"></div>
+
</td> <td class="C1_td_text">
</td> <td class="C1_td_text">
-
<textarea id="i1s4i21" class="C1_text">pbad2m19(45)(65) ai2m19 18ai
+
<textarea id="i1s8i01" class="C1_text">22th September
-
C    C      C        W</textarea>
+
Plasmid:sfGfp(coE1) aiia(pSB3T5)
 +
Bacteria: BL21(WT)
 +
Condition: Flowing medium culture supply. Cells could flow into different traps. With
-
<br> </td> </tr> </tbody></table>
+
antibiotic putting in.
-
 
+
Total Obserbation Time:?
-
</td> </tr> </tbody></table> <div
+
Analysis:  
-
 
+
?
-
class="M1_exp_stepInfo" title="PBAD2M19 1 AND 2" id="sb1s5"> <button
+
</textarea> <br> </td> </tr>
-
 
+
-
class="M1_exp_step_name_button"><input value="PBAD2M19 1 AND 2"
+
-
 
+
-
class="M1_exp_step_name"></button> <span
+
-
 
+
-
class="M1_exp_step_time">2013/8/18 00:00:42--Ruosang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s5i0">2013/8/18 00:01:15</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s5i0"
+
-
 
+
-
class="C9"> <tbody><tr>
+
-
 
+
-
<th colspan="6">Template and Primers</th>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" colspan="3"> choose plasmid </th>
+
-
 
+
-
<th class="C3_td" rowspan="4"> primer1 </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="P1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="pBAD2M19 (7)" class="C3_input"></td> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input value="TATGGCATAGCAAAGTGTG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location
+
-
 
+
-
</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
direction </th> <td
+
-
 
+
-
class="C3_td"> <select id="i1s5i04"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th class="C3_td"
+
-
 
+
-
colspan="2"> type </th> <td
+
-
 
+
-
class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> position
+
-
 
+
-
</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" rowspan="2">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
+
-
 
+
-
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
+
-
 
+
-
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
+
-
 
+
-
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
+
-
 
+
-
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
+
-
 
+
-
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
+
-
 
+
-
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaa
+
-
 
+
-
ccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
+
-
 
+
-
ttacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
+
-
 
+
-
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td"
+
-
 
+
-
rowspan="4"> primer2
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value="P2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td"> length </th>
+
-
 
+
-
<td class="C3_td"><input value="136" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">sequence</th>
+
-
 
+
-
<td class="C3_td"><input value="GCAATATAGTCCATAAGATTAGCGG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">name</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">direction</th> <td
+
-
 
+
-
class="C3_td"> <select id="i1s5i012"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
class="C3_td">length</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s5i1">2013/8/18 00:02:21</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s5i1" class="C9">
+
-
 
+
-
<tbody><tr> <th colspan="6">Template and
+
-
 
+
-
Primers</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3"> choose plasmid
+
-
 
+
-
</th> <th class="C3_td" rowspan="4"> primer1
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th> <td
+
-
 
+
-
class="C3_td"><input value="P3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="pBAD2M19 (7)"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input value="TATGGCATAGCAAAGTGTG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> direction
+
-
 
+
-
</th> <td class="C3_td">
+
-
 
+
-
<select id="i1s5i14" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> position
+
-
 
+
-
</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" rowspan="2">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
+
-
 
+
-
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
+
-
 
+
-
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
+
-
 
+
-
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
+
-
 
+
-
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
+
-
 
+
-
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
+
-
 
+
-
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaa
+
-
 
+
-
ccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
+
-
 
+
-
ttacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
+
-
 
+
-
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td"
+
-
 
+
-
rowspan="4"> primer2
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value="P2" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td"> length </th>
+
-
 
+
-
<td class="C3_td"><input value="136" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">sequence</th>
+
-
 
+
-
<td class="C3_td"><input value="GCAATATAGTCCATAAGATTAGCGG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">name</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">direction</th> <td
+
-
 
+
-
class="C3_td"> <select id="i1s5i112"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
class="C3_td">length</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s5i2">2013/8/18 00:02:55</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s5i2" class="C10">
+
-
 
+
-
<tbody><tr> <th colspan="3">PCR-Reaction</th>
+
-
 
+
-
<td rowspan="9" class="C4_td_textarea">
+
-
 
+
-
<textarea class="C3_textarea" id="i1s5i20">
+
-
 
+
-
</textarea>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Reaction /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="25" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="2.5"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap">dNTP (
+
-
 
+
-
Mixture（2.5 mM each） ) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="4" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="P3" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="1"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap"><input
+
-
 
+
-
value="P2" class="C10_system_input"></th> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">Template /μL <select
+
-
 
+
-
id="i1s5i210">   <option
+
-
 
+
-
value="colony">colony</option>  
+
-
 
+
-
<option value="plasmid">plasmid</option>
+
-
 
+
-
</select> </th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="0.01" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">rTaq /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0.25" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td">H<sub>2</sub>O /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="16.240000000000002" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s5i3">2013/8/18 00:05:05</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s5i3" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s5i30" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s5i313.8.16 pbad2m19 lian1 lian2.jpg"><br>
+
-
 
+
-
<form id="i1s5i34" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i1s5i32"
+
-
 
+
-
form="i1s5i34" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s5i34">
+
-
 
+
-
<input name="id" type="hidden" value="i1s5i3" form="i1s5i34">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s5i35" form="i1s5i34" value="t1i1s5i313.8.16
+
-
 
+
-
pbad2m19 lian1 lian2.jpg"> <input id="i1s5i36" type="submit"
+
-
 
+
-
value="submit" form="i1s5i34" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i1s5i33" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s5i31" class="C1_text">line
+
-
 
+
-
1 and line2</textarea> <br> </td>
+
-
 
+
-
</tr> </tbody></table> </td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s5i4">2013/8/20 00:11:54</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s5i4" class="C10">
+
-
 
+
-
<tbody><tr> <th colspan="3">PCR-Reaction</th>
+
-
 
+
-
<td rowspan="9" class="C4_td_textarea">
+
-
 
+
-
<textarea class="C3_textarea" id="i1s5i40">
+
-
 
+
-
</textarea>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Reaction /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="25" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="2.5"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap">dNTP (
+
-
 
+
-
Mixture（2.5 mM each） ) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="4" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="V1" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="1"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap"><input
+
-
 
+
-
value="V2" class="C10_system_input"></th> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">Template /μL <select
+
-
 
+
-
id="i1s5i410">   <option
+
-
 
+
-
value="colony">colony</option>  
+
-
 
+
-
<option value="plasmid">plasmid</option>
+
-
 
+
-
</select> </th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">rTaq /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0.25" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td">H<sub>2</sub>O /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="6.489999999999998" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s5i5">2013/8/20 00:10:09</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s5i5" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s5i50" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s5i613.8.19 18A-AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN
+
-
 
+
-
(123456).jpg"><br> <form id="i1s5i54" target="M7"
+
-
 
+
-
action="img.php" method="post" enctype="multipart/form-data"></form>
+
-
 
+
-
<input name="img" type="file" id="i1s5i52" form="i1s5i54" class="C1_button1"
+
-
 
+
-
required="required" disabled=""> <input name="pname"
+
-
 
+
-
type="hidden" value="t1" form="i1s5i54"> <input name="id"
+
-
 
+
-
type="hidden" value="i1s5i5" form="i1s5i54"> <input
+
-
 
+
-
name="oldgraph" type="hidden" id="i1s5i55" form="i1s5i54" value="t1i1s5i613.8.19 18A-AI2M19
+
-
 
+
-
M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN(123456).jpg"> <input
+
-
 
+
-
id="i1s5i56" type="submit" value="submit" form="i1s5i54" class="C1_button2" disabled="">
+
-
 
+
-
<div id="i1s5i53" class="M1_exp_img"></div>
+
-
 
+
-
</td> <td class="C1_td_text"> <textarea
+
-
 
+
-
id="i1s5i51" class="C1_text">18A-AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN(123456)
+
-
W  W  W                                      WCCCCC                       
+
-
 
+
-
  WCWWWC</textarea> <br> </td>
+
-
 
+
-
</tr> </tbody></table> </td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s5i6">2013/8/31 11:49:18</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s5i6" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s5i60" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s5i62013.08.31CH.jpg"><br> <form
+
-
 
+
-
id="i1s5i64" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i1s5i62"
+
-
 
+
-
form="i1s5i64" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s5i64">
+
-
 
+
-
<input name="id" type="hidden" value="i1s5i6" form="i1s5i64">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s5i65" form="i1s5i64"
+
-
 
+
-
value="t1i1s5i62013.08.31CH.jpg"> <input id="i1s5i66"
+
-
 
+
-
type="submit" value="submit" form="i1s5i64" class="C1_button2" disabled="">
+
-
 
+
-
<div id="i1s5i63" class="M1_exp_img"></div> </td>
+
-
 
+
-
<td class="C1_td_text"> <textarea id="i1s5i61"
+
-
 
+
-
class="C1_text">4.1  4.2 4.3 9.1 M 9.2 9.3 9.4 9.5 10.1 10.2 10.3 M 14.1 14.2 16.1 16.2 16.3
+
-
 
+
-
17.1 17.2 M 17.2
+
-
C    C    C    C        C    C    C    W  C    C    W      W    C    W 
+
-
 
+
-
W    W  W  W        W
+
-
PBAD LINE0(BL21) LINE1(BL21) LINE2(BL21)
+
-
  C                      C                   
+
-
 
+
-
C</textarea> <br> </td> </tr>
+
</tbody></table> </td> </tr> </tbody></table>
</tbody></table> </td> </tr> </tbody></table>
-
<div class="M1_exp_stepInfo" title="PBAD2M19 4" id="sb1s6">
+
<div class="M1_exp_stepInfo" title="Microfluidic" id="sb1s9">
-
<button class="M1_exp_step_name_button"><input value="PBAD2M19 4"  
+
<button class="M1_exp_step_name_button"><input value="Microfluidic"  
class="M1_exp_step_name"></button> <span  
class="M1_exp_step_name"></button> <span  
-
class="M1_exp_step_time">2013/8/26 00:35:21--Ruosang</span> </div>
+
class="M1_exp_step_time">2013/9/25 21:15:00--Chun Tang</span> </div>
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
-
<td class="M1_exp_item_time" id="ib1s6i0">2013/8/26 00:35:54</td>
+
<td class="M1_exp_item_time" id="ib1s9i0">2013/9/25 21:15:47</td>
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s6i0"
+
-
 
+
-
class="C9"> <tbody><tr>
+
-
 
+
-
<th colspan="6">Template and Primers</th>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" colspan="3"> choose plasmid </th>
+
-
 
+
-
<th class="C3_td" rowspan="4"> primer1 </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="P4" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="pBAD2M19 (7)" class="C3_input"></td> <th
+
-
 
+
-
class="C3_td"> sequence </th>
+
-
 
+
-
<td class="C3_td"><input value="CAAAGTGTGACGCCGTGC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location
+
-
 
+
-
</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
direction </th> <td
+
-
 
+
-
class="C3_td"> <select id="i1s6i04"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th class="C3_td"
+
-
 
+
-
colspan="2"> type </th> <td
+
-
 
+
-
class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> position
+
-
 
+
-
</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" rowspan="2">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
+
-
 
+
-
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
+
-
 
+
-
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
+
-
 
+
-
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
+
-
 
+
-
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
+
-
 
+
-
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
+
-
 
+
-
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaa
+
-
 
+
-
ccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
+
-
 
+
-
ttacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
+
-
 
+
-
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td"
+
-
 
+
-
rowspan="4"> primer2
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value="P5" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td"> length </th>
+
-
 
+
-
<td class="C3_td"><input value="136" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">sequence</th>
+
-
 
+
-
<td class="C3_td"><input value="GCCATAGCAAGATAGTCC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">name</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">direction</th> <td
+
-
 
+
-
class="C3_td"> <select id="i1s6i012"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
class="C3_td">length</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s6i1">2013/8/26 00:37:04</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s6i1" class="C10">
+
-
 
+
-
<tbody><tr> <th colspan="3">PCR-Reaction</th>
+
-
 
+
-
<td rowspan="9" class="C4_td_textarea">
+
-
 
+
-
<textarea class="C3_textarea" id="i1s6i10">
+
-
 
+
-
</textarea>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Reaction /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="50" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="5"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap">dNTP (
+
-
 
+
-
Mixture（2.5 mM each） ) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="4" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="P6" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="1"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap"><input
+
-
 
+
-
value="P5" class="C10_system_input"></th> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">Template /μL <select
+
-
 
+
-
id="i1s6i110">   <option
+
-
 
+
-
value="colony">colony</option>  
+
-
 
+
-
<option value="plasmid">plasmid</option>
+
-
 
+
-
</select> </th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="0.1" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">rTaq /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0.25" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td">H<sub>2</sub>O /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="38.65" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
id="ib1s6i2">2013/8/26 00:40:19</td> <td class="M1_exp_item_content">
+
<td class="M1_exp_item_content"> <table id="i1s9i0" class="C1"> <tbody><tr>
-
<table id="i1s6i2" class="C1"> <tbody><tr> <td class="C1_td">
+
<td class="C1_td"> <img id="i1s9i00"  
-
<img id="i1s6i20" class="C1_graph" src="http://trysomething-
+
class="C1_graph" src="http://trysomething-
-
block.stor.sinaapp.com/t1i1s6i513.8.26pbadl4.l5.jpg"><br>
+
block.stor.sinaapp.com/t14i1s10i0DSC_0455.JPG"><br> <form
-
<form id="i1s6i24" target="M7" action="img.php" method="post" enctype="multipart/form-
+
id="i1s9i04" target="M7" action="img.php" method="post" enctype="multipart/form-
-
data"></form> <input name="img" type="file" id="i1s6i22"  
+
data"></form> <input name="img" type="file" id="i1s9i02"  
-
form="i1s6i24" class="C1_button1" required="required" disabled="">
+
form="i1s9i04" class="C1_button1" required="required" disabled="">
-
<input name="pname" type="hidden" value="t1" form="i1s6i24">
+
<input name="pname" type="hidden" value="t14" form="i1s9i04">
-
<input name="id" type="hidden" value="i1s6i2" form="i1s6i24">
+
<input name="id" type="hidden" value="i1s9i0" form="i1s9i04">
-
<input name="oldgraph" type="hidden" id="i1s6i25" form="i1s6i24"  
+
<input name="oldgraph" type="hidden" id="i1s9i05" form="i1s9i04"  
-
value="t1i1s6i513.8.26pbadl4.l5.jpg"> <input id="i1s6i26"  
+
value="t14i1s10i0DSC_0455.JPG"> <input id="i1s9i06" type="submit"  
-
type="submit" value="submit" form="i1s6i24" class="C1_button2" disabled="">
+
value="submit" form="i1s9i04" class="C1_button2" disabled=""> <div
-
<div id="i1s6i23" class="M1_exp_img"></div> </td>
+
id="i1s9i03" class="M1_exp_img"></div> </td> <td
-
<td class="C1_td_text"> <textarea id="i1s6i21"  
+
class="C1_td_text"> <textarea id="i1s9i01"  
-
class="C1_text">pbad l4.l5</textarea> <br>
+
class="C1_text">Microscope</textarea> <br>
</td> </tr> </tbody></table> </td> </tr>
</td> </tr> </tbody></table> </td> </tr>
Line 16,628: Line 6,992:
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"  
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"  
-
id="ib1s6i3">2013/8/27 00:42:13</td> <td  
+
id="ib1s9i1">2013/9/25 21:16:23</td> <td class="M1_exp_item_content">
-
class="M1_exp_item_content"><textarea id="i1s6i3" class="C0"  
+
<table id="i1s9i1" class="C1"> <tbody><tr> <td class="C1_td">
-
rows="1">TRANSFORMATIOIN</textarea></td> </tr> <tr
+
<img id="i1s9i10" class="C1_graph" src="http://trysomething-
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
block.stor.sinaapp.com/t14i1s10i1wi-part2.jpg"><br> <form
-
id="ib1s6i4">2013/8/27 00:42:54</td> <td class="M1_exp_item_content">
+
id="i1s9i14" target="M7" action="img.php" method="post" enctype="multipart/form-
-
<table id="i1s6i4" class="C1"> <tbody><tr> <td class="C1_td">
+
data"></form> <input name="img" type="file" id="i1s9i12"  
-
<img id="i1s6i40" class="C1_graph" src="http://trysomething-
+
form="i1s9i14" class="C1_button1" required="required" disabled="">
-
block.stor.sinaapp.com/t1i1s6i713 8.29 pbad2m19 line4 (1-4).jpg"><br>
+
<input name="pname" type="hidden" value="t14" form="i1s9i14">
-
<form id="i1s6i44" target="M7" action="img.php" method="post" enctype="multipart/form-
+
<input name="id" type="hidden" value="i1s9i1" form="i1s9i14">
-
data"></form> <input name="img" type="file" id="i1s6i42"  
+
<input name="oldgraph" type="hidden" id="i1s9i15" form="i1s9i14" value="t14i1s10i1wi-
-
form="i1s6i44" class="C1_button1" required="required" disabled="">
+
part2.jpg"> <input id="i1s9i16" type="submit" value="submit"  
-
<input name="pname" type="hidden" value="t1" form="i1s6i44">
+
form="i1s9i14" class="C1_button2" disabled=""> <div id="i1s9i13"  
-
<input name="id" type="hidden" value="i1s6i4" form="i1s6i44">
+
class="M1_exp_img"></div> </td> <td
-
<input name="oldgraph" type="hidden" id="i1s6i45" form="i1s6i44" value="t1i1s6i713 8.29
+
class="C1_td_text"> <textarea id="i1s9i11"  
-
pbad2m19 line4 (1-4).jpg"> <input id="i1s6i46" type="submit"
+
class="C1_text">Microfluidic chip</textarea> <br>
-
 
+
-
value="submit" form="i1s6i44" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i1s6i43" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s6i41"
+
-
 
+
-
class="C1_text">choose the photo you want to submit</textarea> <br>
+
-
 
+
-
</td> </tr> </tbody></table> </td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s6i5">2013/8/28 11:26:54</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s6i5" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s6i50" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s6i513.8.28 pbad2m19 line4 5(1-6).jpg"><br>
+
-
 
+
-
<form id="i1s6i54" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i1s6i52"
+
-
 
+
-
form="i1s6i54" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s6i54">
+
-
 
+
-
<input name="id" type="hidden" value="i1s6i5" form="i1s6i54">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s6i55" form="i1s6i54" value="t1i1s6i513.8.28
+
-
 
+
-
pbad2m19 line4 5(1-6).jpg"> <input id="i1s6i56" type="submit"
+
-
 
+
-
value="submit" form="i1s6i54" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i1s6i53" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s6i51"
+
-
 
+
-
class="C1_text">pbad2m19 line4 5 (1-6)
+
-
WWWWCC  CCCCCC</textarea> <br>
+
</td> </tr> </tbody></table> </td> </tr>
</td> </tr> </tbody></table> </td> </tr>
Line 16,705: Line 7,026:
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"  
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"  
-
id="ib1s6i6">2013/9/16 12:56:58</td> <td  
+
id="ib1s9i2">2013/9/25 21:16:51</td> <td class="M1_exp_item_content">
-
class="M1_exp_item_content"><textarea id="i1s6i6" class="C0" rows="1">THE SEQUNCING SHOWS
+
<table id="i1s9i2" class="C1"> <tbody><tr> <td class="C1_td">
-
THE GENE KNOCKOUT IS SUCCESSFUL TRANSLATE IT INTO BL21</textarea></td> </tr>
+
<img id="i1s9i20" class="C1_graph" src="http://trysomething-
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
block.stor.sinaapp.com/t14i1s10i2wl-part.jpg"><br> <form
-
id="ib1s6i7">2013/9/16 12:58:29</td> <td class="M1_exp_item_content">
+
id="i1s9i24" target="M7" action="img.php" method="post" enctype="multipart/form-
-
<table id="i1s6i7" class="C1"> <tbody><tr> <td class="C1_td">
+
data"></form> <input name="img" type="file" id="i1s9i22"  
-
<img id="i1s6i70" class="C1_graph" src="http://trysomething-
+
form="i1s9i24" class="C1_button1" required="required" disabled="">
-
block.stor.sinaapp.com/t1i1s6i813.9.16 pbad2m19 line4 123456.jpg"><br>
+
<input name="pname" type="hidden" value="t14" form="i1s9i24">
-
<form id="i1s6i74" target="M7" action="img.php" method="post" enctype="multipart/form-
+
<input name="id" type="hidden" value="i1s9i2" form="i1s9i24">
-
data"></form> <input name="img" type="file" id="i1s6i72"  
+
<input name="oldgraph" type="hidden" id="i1s9i25" form="i1s9i24" value="t14i1s10i2wl-
-
form="i1s6i74" class="C1_button1" required="required" disabled="">
+
part.jpg"> <input id="i1s9i26" type="submit" value="submit"  
-
<input name="pname" type="hidden" value="t1" form="i1s6i74">
+
form="i1s9i24" class="C1_button2" disabled=""> <div id="i1s9i23"  
-
<input name="id" type="hidden" value="i1s6i7" form="i1s6i74">
+
class="M1_exp_img"></div> </td> <td
-
<input name="oldgraph" type="hidden" id="i1s6i75" form="i1s6i74" value="t1i1s6i813.9.16
+
class="C1_td_text"> <textarea id="i1s9i21"  
-
pbad2m19 line4 123456.jpg"> <input id="i1s6i76" type="submit"
+
class="C1_text">Valves</textarea> <br>
-
 
+
-
value="submit" form="i1s6i74" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i1s6i73" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s6i71"
+
-
 
+
-
class="C1_text">PBAD2M19 LINE 4 IN BL21  123456  ALL CORRECT</textarea>
+
-
 
+
-
<br> </td> </tr> </tbody></table> </td>
+
-
 
+
-
</tr> </tbody></table> <div class="M1_exp_stepInfo"
+
-
 
+
-
title="PBAD2M10 5" id="sb1s7"> <button
+
-
 
+
-
class="M1_exp_step_name_button"><input value="PBAD2M10 5" class="M1_exp_step_name"></button>
+
-
 
+
-
<span class="M1_exp_step_time">2013/8/26 00:39:17--Ruosang</span>
+
-
 
+
-
</div> <table class="M1_exp_stepBoard"> <tbody><tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s7i0">2013/8/26 00:39:25</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s7i0" class="C9">
+
-
 
+
-
<tbody><tr> <th colspan="6">Template and
+
-
 
+
-
Primers</th> </tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="3"> choose plasmid
+
-
 
+
-
</th> <th class="C3_td" rowspan="4"> primer1
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th> <td
+
-
 
+
-
class="C3_td"><input value="P6" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" rowspan="2"> plasmid </th>
+
-
 
+
-
<th class="C3_td"> name </th>
+
-
 
+
-
<td class="C3_td"><input value="pBAD2M19 (7)"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input value="AGCAAAGTGTGACGCCGTG" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td"> location </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> direction
+
-
 
+
-
</th> <td class="C3_td">
+
-
 
+
-
<select id="i1s7i04" class="C3_input">
+
-
 
+
-
  <option value="left">left</option>
+
-
 
+
-
  <option value="right">right</option>
+
-
 
+
-
</select> </td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" colspan="2"> type </th>
+
-
 
+
-
<td class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td"> position
+
-
 
+
-
</th> <td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" rowspan="2">
+
-
 
+
-
part-only
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
sequence </th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
+
-
 
+
-
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
+
-
 
+
-
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
+
-
 
+
-
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
+
-
 
+
-
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
+
-
 
+
-
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
+
-
 
+
-
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaa
+
-
 
+
-
ccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
+
-
 
+
-
ttacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
+
-
 
+
-
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata"
+
-
 
+
-
class="C3_input"></td> <th class="C3_td"
+
-
 
+
-
rowspan="4"> primer2
+
-
 
+
-
</th> <th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value="P5" class="C3_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td"> length </th>
+
-
 
+
-
<td class="C3_td"><input value="136" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">sequence</th>
+
-
 
+
-
<td class="C3_td"><input value="GCCATAGCAAGATAGTCC" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" rowspan="2">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">name</th> <td
+
-
 
+
-
class="C3_td"><input value="psb1a2" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">direction</th> <td
+
-
 
+
-
class="C3_td"> <select id="i1s7i012"
+
-
 
+
-
class="C3_input">   <option
+
-
 
+
-
value="left">left</option>   <option
+
-
 
+
-
value="right">right</option> </select>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
class="C3_td">length</th> <td
+
-
 
+
-
class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
<th class="C3_td">position</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s7i1">2013/8/26 00:40:04</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s7i1" class="C10">
+
-
 
+
-
<tbody><tr> <th colspan="3">PCR-Reaction</th>
+
-
 
+
-
<td rowspan="9" class="C4_td_textarea">
+
-
 
+
-
<textarea class="C3_textarea" id="i1s7i10">
+
-
 
+
-
</textarea>
+
-
 
+
-
</td> </tr> <tr>
+
-
 
+
-
<th class="C3_td">Reaction /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="50" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="5"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap">dNTP (
+
-
 
+
-
Mixture（2.5 mM each） ) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="4" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="P6" class="C10_system_input"></th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="1"
+
-
 
+
-
class="C10_input"></td> </tr>
+
-
 
+
-
<tr> <th class="C3_td" nowrap="nowrap"><input
+
-
 
+
-
value="P5" class="C10_system_input"></th> <td
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">Template /μL <select
+
-
 
+
-
id="i1s7i110">   <option
+
-
 
+
-
value="colony">colony</option>  
+
-
 
+
-
<option value="plasmid">plasmid</option>
+
-
 
+
-
</select> </th>
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="0.1" class="C10_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">rTaq /μL</th>
+
-
 
+
-
<td class="C3_td"><input value="0.25" class="C10_input"></td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
class="C3_td">H<sub>2</sub>O /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="38.65" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s7i2">2013/8/26 00:40:51</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s7i2" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s7i20" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s7i213.8.26pbadl4.l5.jpg"><br>
+
-
 
+
-
<form id="i1s7i24" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i1s7i22"
+
-
 
+
-
form="i1s7i24" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s7i24">
+
-
 
+
-
<input name="id" type="hidden" value="i1s7i2" form="i1s7i24">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s7i25" form="i1s7i24"
+
-
 
+
-
value="t1i1s7i213.8.26pbadl4.l5.jpg"> <input id="i1s7i26"
+
-
 
+
-
type="submit" value="submit" form="i1s7i24" class="C1_button2" disabled="">
+
-
 
+
-
<div id="i1s7i23" class="M1_exp_img"></div> </td>
+
-
 
+
-
<td class="C1_td_text"> <textarea id="i1s7i21"
+
-
 
+
-
class="C1_text">pbad l4.l5</textarea> <br>
+
-
 
+
-
</td> </tr> </tbody></table> </td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s7i3">2013/8/27 00:42:25</td> <td
+
-
 
+
-
class="M1_exp_item_content"><textarea id="i1s7i3" class="C0"
+
-
 
+
-
rows="1">TRANSFORMATION</textarea></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s7i4">2013/8/28 11:27:57</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s7i4" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s7i40" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s7i413.8.28 pbad2m19 line4 5(1-6).jpg"><br>
+
-
 
+
-
<form id="i1s7i44" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i1s7i42"
+
-
 
+
-
form="i1s7i44" class="C1_button1" required="required" disabled="">
+
-
 
+
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<input name="pname" type="hidden" value="t1" form="i1s7i44">
+
-
 
+
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<input name="id" type="hidden" value="i1s7i4" form="i1s7i44">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s7i45" form="i1s7i44" value="t1i1s7i413.8.28
+
-
 
+
-
pbad2m19 line4 5(1-6).jpg"> <input id="i1s7i46" type="submit"
+
-
 
+
-
value="submit" form="i1s7i44" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i1s7i43" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s7i41"
+
-
 
+
-
class="C1_text">pbad2m19 line4 5 (1-6)
+
-
WWWWCC  CCCCCC</textarea> <br>
+
</td> </tr> </tbody></table> </td> </tr>
</td> </tr> </tbody></table> </td> </tr>
-
</tbody></table> <div class="M1_exp_stepInfo" title="LINE
+
</tbody></table>
-
 
+
-
0,1,2 IN C3" id="sb1s8"> <button
+
-
 
+
-
class="M1_exp_step_name_button"><input value="LINE 0,1,2 IN C3"
+
-
 
+
-
class="M1_exp_step_name"></button> <span
+
-
 
+
-
class="M1_exp_step_time">2013/9/6 12:40:40--Ruosang</span> </div>
+
-
 
+
-
<table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem">
+
-
 
+
-
<td class="M1_exp_item_time" id="ib1s8i0">2013/9/6 12:41:24</td>
+
-
 
+
-
<td class="M1_exp_item_content"> <table id="i1s8i0" class="C8">
+
-
 
+
-
<tbody><tr> <th colspan="2"
+
-
 
+
-
class="C8_td_title"> Start to Ligate Plasmids
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (A)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="C3" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="BACKBONE"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
backbone </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
</tbody></table>
+
-
 
+
-
</td> <td>
+
-
 
+
-
<table class="C8"> <tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (B)
+
-
 
+
-
</th> </tr>
+
-
 
+
-
<tr> <th
+
-
 
+
-
rowspan="2" class="C3_td">
+
-
 
+
-
plasmid </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="pBAD2M19" class="C3_input"></td>
+
-
 
+
-
</tr> <tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
type </th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="pbad-rbs-gfp-tt"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th rowspan="2" class="C3_td">
+
-
 
+
-
part-only </th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td
+
-
 
+
-
class="C3_td"><input
+
-
 
+
-
value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgcttttt
+
-
 
+
-
atcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaat
+
-
 
+
-
tcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
+
-
 
+
-
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
+
-
 
+
-
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
+
-
 
+
-
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
+
-
 
+
-
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
+
-
 
+
-
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaa
+
-
 
+
-
ccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
+
-
 
+
-
ttacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
+
-
 
+
-
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="993" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> backbone
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value="psb1a2"
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="2079" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
</tbody></table> </td>
+
-
 
+
-
<td style="display: none;"> <table class="C8">
+
-
 
+
-
<tbody><tr>
+
-
 
+
-
<th colspan="3" class="C8_td_title">
+
-
 
+
-
choose plasmid (C) </th>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> plasmid
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
location </th>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td"> type
+
-
 
+
-
</th>
+
-
 
+
-
<td class="C3_td" colspan="2"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> part-only
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
sequence
+
-
 
+
-
</th> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
<tr> <th rowspan="2"
+
-
 
+
-
class="C3_td"> backbone
+
-
 
+
-
</th>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
name </th>
+
-
 
+
-
<td class="C3_td"><input value=""
+
-
 
+
-
class="C3_input"></td> </tr>
+
-
 
+
-
<tr>
+
-
 
+
-
<th class="C3_td">
+
-
 
+
-
length </th>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr>
+
-
 
+
-
</tbody></table> </td>
+
-
 
+
-
</tr> <tr> <td class="C3_td"
+
-
 
+
-
colspan="2"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you need another plasmid (C) , double click here"
+
-
 
+
-
name="#i1s8i0"> <input type="button"
+
-
 
+
-
class="C8_button" value="if you don't need the plasmid (C) , double click here"
+
-
 
+
-
name="#i1s8i0" style="display: none;"> </td>
+
-
 
+
-
</tr> </tbody></table></td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s8i1">2013/9/6 12:42:40</td> <td
+
-
 
+
-
class="M1_exp_item_content"><textarea id="i1s8i1" class="C0" rows="1">DIGESTE BY 5uL EcoRI
+
-
 
+
-
AND PstI</textarea></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s8i2">2013/9/6 12:43:17</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s8i2" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s8i20" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s8i313.9.6 C3 LINE0 LINE1 LINE2.jpg"><br>
+
-
 
+
-
<form id="i1s8i24" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i1s8i22"
+
-
 
+
-
form="i1s8i24" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s8i24">
+
-
 
+
-
<input name="id" type="hidden" value="i1s8i2" form="i1s8i24">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s8i25" form="i1s8i24" value="t1i1s8i313.9.6 C3
+
-
 
+
-
LINE0 LINE1 LINE2.jpg"> <input id="i1s8i26" type="submit"
+
-
 
+
-
value="submit" form="i1s8i24" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i1s8i23" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s8i21" class="C1_text">C3
+
-
 
+
-
LINE0 LINE1 LINE2
+
-
C  W          C        W</textarea> <br>
+
-
 
+
-
</td> </tr> </tbody></table> </td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s8i3">2013/9/6 12:44:27</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s8i3" class="C6"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="5">Electrophoretogram and
+
-
 
+
-
recover</th> <td rowspan="10" class="C6_td_textarea">
+
-
 
+
-
<textarea class="C3_textarea" id="i1s8i36">
+
-
 
+
-
</textarea> </td>
+
-
 
+
-
</tr> <tr> <th
+
-
 
+
-
colspan="5" class="C3_td"> Analyse
+
-
 
+
-
Electrophoretogram </th>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct1s8i3">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">A<sub>260/280</sub></th> <th
+
-
 
+
-
class="C3_td">ng/μL</th> <th class="C3_td">bp</th>
+
-
 
+
-
<td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Correct" class="C6_button" name="1s8i3">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Correct1s8i3"> <td class="C3_td"
+
-
 
+
-
nowrap="nowrap">(A)</td> <td class="C3_td"><input
+
-
 
+
-
value="" class="C3_input"></td> <td class="C3_td"><input
+
-
 
+
-
value="38.1" class="C6_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="2070" class="C6_input"></td>
+
-
 
+
-
</tr> <tr class="mdoelC5Correct1s8i3">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap">(B)</td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="8.4" class="C6_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="993" class="C6_input"></td> </tr>
+
-
 
+
-
<tr class="mdoelC5Correct1s8i3" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap">(C)</td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="mdoelC5Wrong1s8i3" style="display: none;">
+
-
 
+
-
<td class="C3_td" colspan="4" rowspan="3">
+
-
 
+
-
Possible reason:<br> 1. The validity or
+
-
 
+
-
contamination of gel extraction kit and DD water.<br>
+
-
 
+
-
2. The validity of enzyme and buffer<br> 3.
+
-
 
+
-
Incomplete dissolution of agar gel<br> </td>
+
-
 
+
-
<td class="C3_td" rowspan="4">
+
-
 
+
-
<input type="button" value="Wrong" class="C6_button" name="1s8i3">
+
-
 
+
-
</td> </tr> <tr
+
-
 
+
-
class="mdoelC5Wrong1s8i3" style="display: none;"> </tr>
+
-
 
+
-
<tr class="mdoelC5Wrong1s8i3" style="display: none;">
+
-
 
+
-
</tr> <tr class="mdoelC5Wrong1s8i3" style="display: none;">
+
-
 
+
-
</tr> </tbody></table> <input
+
-
 
+
-
type="hidden" id="i1s8i37" value="1"></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s8i4">2013/9/6 12:44:44</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s8i4" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s8i40"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i1s8i426">A
+
-
 
+
-
(vector) /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="1.0627720362233637" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
id="i1s8i427">B (insert) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="6.937227963776635" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="7" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<th class="C3_td">ligase /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s8i5">2013/9/7 12:48:34</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s8i5" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s8i50" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s8i613.9.7 pbad2m19 line0 line2.jpg"><br>
+
-
 
+
-
<form id="i1s8i54" target="M7" action="img.php" method="post" enctype="multipart/form-
+
-
 
+
-
data"></form> <input name="img" type="file" id="i1s8i52"
+
-
 
+
-
form="i1s8i54" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s8i54">
+
-
 
+
-
<input name="id" type="hidden" value="i1s8i5" form="i1s8i54">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s8i55" form="i1s8i54" value="t1i1s8i613.9.7
+
-
 
+
-
pbad2m19 line0 line2.jpg"> <input id="i1s8i56" type="submit"
+
-
 
+
-
value="submit" form="i1s8i54" class="C1_button2" disabled=""> <div
+
-
 
+
-
id="i1s8i53" class="M1_exp_img"></div> </td> <td
+
-
 
+
-
class="C1_td_text"> <textarea id="i1s8i51" class="C1_text">0 AND
+
-
 
+
-
2</textarea> <br> </td> </tr>
+
-
 
+
-
</tbody></table> </td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s8i6">2013/9/7 12:49:01</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s8i6" class="C7"> <tbody><tr>
+
-
 
+
-
<th class="C3_td_title" colspan="3">
+
-
 
+
-
Ligation </th>
+
-
 
+
-
<td rowspan="8" class="C3_td_textarea"> <textarea
+
-
 
+
-
class="C3_textarea" id="i1s8i60"> </textarea>
+
-
 
+
-
</td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
nowrap="nowrap"><input value="10" class="C7_blur_1"></th>
+
-
 
+
-
<td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<td class="C3_td"></td> <th
+
-
 
+
-
class="C3_td">Recommend</th> <th class="C3_td">Actual
+
-
 
+
-
use</th> </tr> <tr
+
-
 
+
-
class="C7_tr_AB"> <th class="C3_td" id="i1s8i626">A
+
-
 
+
-
(vector) /μL</th> <td class="C3_td"><input
+
-
 
+
-
value="1.0627720362233637" class="C3_input"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_AB"> <th class="C3_td"
+
-
 
+
-
id="i1s8i627">B (insert) /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="6.937227963776635" class="C3_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="7" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB"> <th
+
-
 
+
-
class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_blur_2"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_AB">
+
-
 
+
-
<th class="C3_td">ligase /μL</th> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C7_blur_2"></td> <td
+
-
 
+
-
class="C3_td"><input value="1" class="C3_input"></td> </tr>
+
-
 
+
-
+
-
 
+
-
<tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <th class="C3_td" nowrap="nowrap"><input value="25"
+
-
 
+
-
class="C5_system_input"></th> <td
+
-
 
+
-
class="C3_td">Recommend</td> <td class="C3_td">Actual
+
-
 
+
-
use</td> </tr> <tr
+
-
 
+
-
class="C7_tr_ABC" style="display: none;"> <td
+
-
 
+
-
class="C3_td">A /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">B /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">C /μL</td> <td class="C3_td"><input
+
-
 
+
-
value="1" class="C7_input_C"></td> <td
+
-
 
+
-
class="C3_td"><input value="" class="C3_input"></td> </tr>
+
-
 
+
-
<tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display:
+
-
 
+
-
none;"> <td class="C3_td">ligase /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="1" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> <tr class="C7_tr_ABC" style="display: none;">
+
-
 
+
-
<td class="C3_td">H<sub>2</sub>O /μL</td>
+
-
 
+
-
<td class="C3_td"><input value="20" class="C7_input_C"></td>
+
-
 
+
-
<td class="C3_td"><input value="" class="C3_input"></td>
+
-
 
+
-
</tr> </tbody></table></td> </tr> <tr
+
-
 
+
-
class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s8i7">2013/9/7 12:49:19</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s8i7" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s8i70" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s8i813.9.8 c3-line1(1) c3-line1(2) 2M19D5 2M19D5 500.jpg"><br>
+
-
 
+
-
<form id="i1s8i74" target="M7" action="img.php" method="post"
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"
+
-
 
+
-
type="file" id="i1s8i72" form="i1s8i74" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s8i74">
+
-
 
+
-
<input name="id" type="hidden" value="i1s8i7" form="i1s8i74">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s8i75" form="i1s8i74"
+
-
 
+
-
value="t1i1s8i813.9.8 c3-line1(1) c3-line1(2) 2M19D5 2M19D5 500.jpg">
+
-
 
+
-
<input id="i1s8i76" type="submit" value="submit" form="i1s8i74" class="C1_button2"
+
-
 
+
-
disabled=""> <div id="i1s8i73" class="M1_exp_img"></div>
+
-
 
+
-
</td> <td class="C1_td_text">
+
-
 
+
-
<textarea id="i1s8i71" class="C1_text">LINE 1 IN C3  OTHER SAMPLE  M
+
-
C    C</textarea> <br>
+
-
 
+
-
</td> </tr> </tbody></table> </td> </tr>
+
-
 
+
-
<tr class="M1_exp_stepItem"> <td class="M1_exp_item_time"
+
-
 
+
-
id="ib1s8i8">2013/9/9 12:50:45</td> <td class="M1_exp_item_content">
+
-
 
+
-
<table id="i1s8i8" class="C1"> <tbody><tr> <td class="C1_td">
+
-
 
+
-
<img id="i1s8i80" class="C1_graph" src="http://trysomething-
+
-
 
+
-
block.stor.sinaapp.com/t1i1s8i913.9.9 pbad2m19-c3 line0 1 2 ,line2 1 2.jpg"><br>
+
-
 
+
-
<form id="i1s8i84" target="M7" action="img.php" method="post"
+
-
 
+
-
enctype="multipart/form-data"></form> <input name="img"
+
-
 
+
-
type="file" id="i1s8i82" form="i1s8i84" class="C1_button1" required="required" disabled="">
+
-
 
+
-
<input name="pname" type="hidden" value="t1" form="i1s8i84">
+
-
 
+
-
<input name="id" type="hidden" value="i1s8i8" form="i1s8i84">
+
-
 
+
-
<input name="oldgraph" type="hidden" id="i1s8i85" form="i1s8i84"
+
-
 
+
-
value="t1i1s8i913.9.9 pbad2m19-c3 line0 1 2 ,line2 1 2.jpg">
+
-
 
+
-
<input id="i1s8i86" type="submit" value="submit" form="i1s8i84" class="C1_button2"
+
-
 
+
-
disabled=""> <div id="i1s8i83" class="M1_exp_img"></div>
+
-
 
+
-
</td> <td class="C1_td_text">
+
-
 
+
-
<textarea id="i1s8i81" class="C1_text">ANALYSE LINE 0  1，2  M  LINE 2  1，2</textarea>
+
-
 
+
-
<br> </td> </tr> </tbody></table>
+
-
</td> </tr> </tbody></table>
 
-
</div>
 
-
</div>
 
-
</div>
 
-
</div>
 
<script>
<script>
var dataOutput_tetetetete=-1;
var dataOutput_tetetetete=-1;
Line 17,914: Line 7,083:
}
}
});
});
-
</script>
+
</script>
-
</body>
+
</html>
</html>

Revision as of 16:35, 25 September 2013

18A-AI-2M19
2013/7/29 09:15:49--Ruosang
2013/7/29 09:16:24
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/7/30 09:18:19
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/7/30 09:19:34


2013/7/30 09:21:18
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/7/30 09:21:31


2013/7/30 09:22:23
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/7/30 09:23:12
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
Recommend Actual use
ligase /μL
2013/7/31 09:23:55
2013/8/1 09:24:26--Ruosang
2013/8/1 09:24:42
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/1 09:25:15
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/1 09:25:39


2013/8/5 21:44:19--Ruosang
2013/8/5 21:45:25
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/5 21:45:55


2013/8/6 21:46:26
2013/8/7 21:50:17
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/7 21:50:56
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/7 21:52:12
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/7 21:52:52
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/8 21:54:37


2013/8/9 22:05:07


2013/8/9 22:13:49
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/9 22:14:05
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/16 23:58:07
2013/8/7 21:46:54--Ruosang
2013/8/7 21:47:35
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/7 21:48:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/7 21:48:47


2013/8/9 22:02:31


2013/8/9 22:15:05
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/9 22:15:18
Cultivation
Plasmid (A )
Strain
antibiotics
Medium
Volume
Temperature ℃
Speed
Plasmid (B )
Strain
antibiotics
Medium
Volume
Temperature ℃
Speed
Plasmid (C )
Strain
antibiotics
Medium
Volume
Temperature ℃
Speed
2013/8/10 22:16:21
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/16 23:58:45
2013/8/8 21:56:45--Ruosang
2013/8/8 21:57:12
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/8 22:08:20


2013/8/9 22:09:20--Ruosang
2013/8/9 22:09:38
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/9 22:10:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/9 22:11:10
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/9 22:11:57


2013/8/12 22:22:06--Ruosang
2013/8/12 22:22:24
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/12 22:22:43
Cultivation
Plasmid (A )
Strain
antibiotics
Medium
Volume
Temperature ℃
Speed
Plasmid (B )
Strain
antibiotics
Medium
Volume
Temperature ℃
Speed
Plasmid (C )
Strain
antibiotics
Medium
Volume
Temperature ℃
Speed
2013/8/13 23:21:13
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/13 23:21:47


2013/8/13 23:25:17
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/13 23:36:12


2013/8/15 23:41:26--Ruosang
2013/8/15 23:41:42
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
2013/9/18 10:50:50--Chun Tang
2013/9/25 20:38:47


2013/9/18 10:51:01--Chun Tang
2013/9/25 20:42:38


2013/9/18 10:51:41--Chun Tang
2013/9/25 20:45:15


2013/9/18 10:52:00--Chun Tang
2013/9/25 20:46:04


2013/9/18 10:52:31--Chun Tang
2013/9/25 20:47:42


2013/9/18 10:53:02--Chun Tang
2013/9/25 20:50:35


2013/9/18 10:53:24--Chun Tang
2013/9/25 20:52:02


2013/9/25 21:10:34--Chun Tang
2013/9/25 21:11:06


2013/9/25 21:12:48--Chun Tang
2013/9/25 21:13:01


2013/9/25 21:15:00--Chun Tang
2013/9/25 21:15:47


2013/9/25 21:16:23


2013/9/25 21:16:51


Retrieved from "http://2013.igem.org/Team:XMU-China/wetlab_journal"