Team:XMU Software/Project protein/cutting site

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Revision as of 00:53, 29 October 2013

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14 kinds of commonly used cutting sites

Enzyme cutting site Recognition sequence
ApaI 5’GGGCC^C’3
BamHI 5’G^GATCC’3
BglII 5’A^GATCT’3
EcoRI 5’G^AATTC’3
Hind1II 5’A^AGCTT’3
KpnI 5’GGTAC^C’3
NcoI 5’C^CATGG’3
NdeI 5’CA^TATG’3
NheI 5’G^CTAGC’3
NotI 5’GC^GGCCGC’3
Sa1I 5’G^TCGAC’3
SphI 5’GCATG^C’3
XbaI 5’T^CTAGA’3
XhoI 5’C^TCGAG’3