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| | <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.8.0/jquery.min.js"></script> | | <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.8.0/jquery.min.js"></script> |
| | <script type="text/javascript" async="" src="https://2013.igem.org/wiki/index.php?title=Team:XMU_Software/js&action=raw&ctype=text/javascript"></script> | | <script type="text/javascript" async="" src="https://2013.igem.org/wiki/index.php?title=Team:XMU_Software/js&action=raw&ctype=text/javascript"></script> |
| - | <link rel="stylesheet" type="text/css" href="xml.css"/> | + | <link rel="stylesheet" type="text/css" href="https://2013.igem.org/wiki/index.php?title=Team:XMU_Software/css&action=raw&ctype=text/css"/> |
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| | W W W WCCCCC WCWWWC</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18A-AI2M19" id="sb0s8"> <button class="M1_exp_step_name_button"><input value="18A-AI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/20 00:14:31--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i0">2013/8/20 00:14:48</td> <td class="M1_exp_item_content"> <table id="i0s8i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="35" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s8i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s8i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i1">2013/8/20 00:15:21</td> <td class="M1_exp_item_content"> <table id="i0s8i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s8i10" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s10i113.8.19 18A-AI2M19 1 2.jpg"><br> <form id="i0s8i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s8i12" form="i0s8i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s8i14"> <input name="id" type="hidden" value="i0s8i1" form="i0s8i14"> <input name="oldgraph" type="hidden" id="i0s8i15" form="i0s8i14" value="t1i0s10i113.8.19 18A-AI2M19 1 2.jpg"> <input id="i0s8i16" type="submit" value="submit" form="i0s8i14" class="C1_button2" disabled=""> <div id="i0s8i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s8i11" class="C1_text">choose the photo you want to submit</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i2">2013/8/20 00:16:34</td> <td class="M1_exp_item_content"> <table id="i0s8i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s8i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s10i213.8.19 18A.jpg"><br> <form id="i0s8i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s8i22" form="i0s8i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s8i24"> <input name="id" type="hidden" value="i0s8i2" form="i0s8i24"> <input name="oldgraph" type="hidden" id="i0s8i25" form="i0s8i24" value="t1i0s10i213.8.19 18A.jpg"> <input id="i0s8i26" type="submit" value="submit" form="i0s8i24" class="C1_button2" disabled=""> <div id="i0s8i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s8i21" class="C1_text">choose the photo you want to submit</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i3">2013/8/20 00:16:50</td> <td class="M1_exp_item_content"> <table id="i0s8i3" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s8i30"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s8i326">A (vector) /μL</th> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s8i327">B (insert) /μL</th> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="7" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18A-AI2M19" id="sb0s9"> <button class="M1_exp_step_name_button"><input value="18A-AI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/21 00:17:26--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s9i0">2013/8/21 00:17:45</td> <td class="M1_exp_item_content"> <table id="i0s9i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="35" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="35" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s9i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s9i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s9i1">2013/8/21 00:18:04</td> <td class="M1_exp_item_content"> <table id="i0s9i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i0s9i19"> </textarea></td> </tr> <tr> <td class="C3_td"> </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.87" class="C3_input"></td> <td class="C3_td"><input value="89.6" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="1.68" class="C3_input"></td> <td class="C3_td"><input value="107.2" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i0s9i112" name="#i0s9i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18O-AI2M19" id="sb0s10"> <button class="M1_exp_step_name_button"><input value="18O-AI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/22 00:18:33--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s10i0">2013/8/22 00:18:50</td> <td class="M1_exp_item_content"> <table id="i0s10i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="0" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s10i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s10i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s10i1">2013/8/22 00:19:07</td> <td class="M1_exp_item_content"> <table id="i0s10i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s10i10" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s12i113.8.22 18o ai2m19 18o1 s d 18o2s.jpg"><br> <form id="i0s10i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s10i12" form="i0s10i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s10i14"> <input name="id" type="hidden" value="i0s10i1" form="i0s10i14"> <input name="oldgraph" type="hidden" id="i0s10i15" form="i0s10i14" value="t1i0s12i113.8.22 18o ai2m19 18o1 s d 18o2s.jpg"> <input id="i0s10i16" type="submit" value="submit" form="i0s10i14" class="C1_button2" disabled=""> <div id="i0s10i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s10i11" class="C1_text">18o ai2m19 18o1 s d 18o2s | | W W W WCCCCC WCWWWC</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18A-AI2M19" id="sb0s8"> <button class="M1_exp_step_name_button"><input value="18A-AI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/20 00:14:31--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i0">2013/8/20 00:14:48</td> <td class="M1_exp_item_content"> <table id="i0s8i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="35" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s8i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s8i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i1">2013/8/20 00:15:21</td> <td class="M1_exp_item_content"> <table id="i0s8i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s8i10" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s10i113.8.19 18A-AI2M19 1 2.jpg"><br> <form id="i0s8i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s8i12" form="i0s8i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s8i14"> <input name="id" type="hidden" value="i0s8i1" form="i0s8i14"> <input name="oldgraph" type="hidden" id="i0s8i15" form="i0s8i14" value="t1i0s10i113.8.19 18A-AI2M19 1 2.jpg"> <input id="i0s8i16" type="submit" value="submit" form="i0s8i14" class="C1_button2" disabled=""> <div id="i0s8i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s8i11" class="C1_text">choose the photo you want to submit</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i2">2013/8/20 00:16:34</td> <td class="M1_exp_item_content"> <table id="i0s8i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s8i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s10i213.8.19 18A.jpg"><br> <form id="i0s8i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s8i22" form="i0s8i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s8i24"> <input name="id" type="hidden" value="i0s8i2" form="i0s8i24"> <input name="oldgraph" type="hidden" id="i0s8i25" form="i0s8i24" value="t1i0s10i213.8.19 18A.jpg"> <input id="i0s8i26" type="submit" value="submit" form="i0s8i24" class="C1_button2" disabled=""> <div id="i0s8i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s8i21" class="C1_text">choose the photo you want to submit</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s8i3">2013/8/20 00:16:50</td> <td class="M1_exp_item_content"> <table id="i0s8i3" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s8i30"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s8i326">A (vector) /μL</th> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s8i327">B (insert) /μL</th> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="7" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18A-AI2M19" id="sb0s9"> <button class="M1_exp_step_name_button"><input value="18A-AI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/21 00:17:26--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s9i0">2013/8/21 00:17:45</td> <td class="M1_exp_item_content"> <table id="i0s9i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="35" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="35" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s9i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s9i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s9i1">2013/8/21 00:18:04</td> <td class="M1_exp_item_content"> <table id="i0s9i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i0s9i19"> </textarea></td> </tr> <tr> <td class="C3_td"> </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.87" class="C3_input"></td> <td class="C3_td"><input value="89.6" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="1.68" class="C3_input"></td> <td class="C3_td"><input value="107.2" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i0s9i112" name="#i0s9i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18O-AI2M19" id="sb0s10"> <button class="M1_exp_step_name_button"><input value="18O-AI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/22 00:18:33--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s10i0">2013/8/22 00:18:50</td> <td class="M1_exp_item_content"> <table id="i0s10i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="0" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s10i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s10i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s10i1">2013/8/22 00:19:07</td> <td class="M1_exp_item_content"> <table id="i0s10i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s10i10" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s12i113.8.22 18o ai2m19 18o1 s d 18o2s.jpg"><br> <form id="i0s10i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s10i12" form="i0s10i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s10i14"> <input name="id" type="hidden" value="i0s10i1" form="i0s10i14"> <input name="oldgraph" type="hidden" id="i0s10i15" form="i0s10i14" value="t1i0s12i113.8.22 18o ai2m19 18o1 s d 18o2s.jpg"> <input id="i0s10i16" type="submit" value="submit" form="i0s10i14" class="C1_button2" disabled=""> <div id="i0s10i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s10i11" class="C1_text">18o ai2m19 18o1 s d 18o2s |
| | C</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s10i2">2013/8/22 00:20:05</td> <td class="M1_exp_item_content"> <table class="C5" id="i0s10i2"> <tbody><tr> <th colspan="3" id="i0s10i229" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s10i20"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i0s10i230"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i0s10i21" name="#i0s10i2" value="18O (A)"> <input type="button" class="C5_canvas_button2" id="i0s10i22" name="#i0s10i2" value="AI2M19 (B)"> <input type="button" class="C5_canvas_button3" id="i0s10i23" name="#i0s10i2" value="18O (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="50" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="30" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i0s10i226" value="0"> <input type="hidden" id="i0s10i227" value="1"> <input type="hidden" id="i0s10i228" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s10i3">2013/8/22 00:20:33</td> <td class="M1_exp_item_content"> <table id="i0s10i3" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s10i30"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s10i326">A (vector) /μL</th> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s10i327">B (insert) /μL</th> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="7" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18O AND 18A" id="sb0s11"> <button class="M1_exp_step_name_button"><input value="18O AND 18A" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/23 00:21:17--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s11i0">2013/8/23 00:21:30</td> <td class="M1_exp_item_content"> <table id="i0s11i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="35" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="0" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s11i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s11i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s11i1">2013/8/23 00:21:48</td> <td class="M1_exp_item_content"> <table id="i0s11i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s11i10" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s13i113.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"><br> <form id="i0s11i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s11i12" form="i0s11i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s11i14"> <input name="id" type="hidden" value="i0s11i1" form="i0s11i14"> <input name="oldgraph" type="hidden" id="i0s11i15" form="i0s11i14" value="t1i0s13i113.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"> <input id="i0s11i16" type="submit" value="submit" form="i0s11i14" class="C1_button2" disabled=""> <div id="i0s11i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s11i11" class="C1_text">18o-ai2m19 1 2 3 M 18A 1 2 | | C</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s10i2">2013/8/22 00:20:05</td> <td class="M1_exp_item_content"> <table class="C5" id="i0s10i2"> <tbody><tr> <th colspan="3" id="i0s10i229" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s10i20"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i0s10i230"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i0s10i21" name="#i0s10i2" value="18O (A)"> <input type="button" class="C5_canvas_button2" id="i0s10i22" name="#i0s10i2" value="AI2M19 (B)"> <input type="button" class="C5_canvas_button3" id="i0s10i23" name="#i0s10i2" value="18O (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="50" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="30" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i0s10i226" value="0"> <input type="hidden" id="i0s10i227" value="1"> <input type="hidden" id="i0s10i228" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s10i3">2013/8/22 00:20:33</td> <td class="M1_exp_item_content"> <table id="i0s10i3" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s10i30"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s10i326">A (vector) /μL</th> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s10i327">B (insert) /μL</th> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="7" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18O AND 18A" id="sb0s11"> <button class="M1_exp_step_name_button"><input value="18O AND 18A" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/23 00:21:17--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s11i0">2013/8/23 00:21:30</td> <td class="M1_exp_item_content"> <table id="i0s11i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18A" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="35" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="0" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s11i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s11i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s11i1">2013/8/23 00:21:48</td> <td class="M1_exp_item_content"> <table id="i0s11i1" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s11i10" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s13i113.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"><br> <form id="i0s11i14" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s11i12" form="i0s11i14" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s11i14"> <input name="id" type="hidden" value="i0s11i1" form="i0s11i14"> <input name="oldgraph" type="hidden" id="i0s11i15" form="i0s11i14" value="t1i0s13i113.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"> <input id="i0s11i16" type="submit" value="submit" form="i0s11i14" class="C1_button2" disabled=""> <div id="i0s11i13" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s11i11" class="C1_text">18o-ai2m19 1 2 3 M 18A 1 2 |
| - | WCC C W</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18OAI2M19" id="sb0s12"> <button class="M1_exp_step_name_button"><input value="18OAI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/23 00:23:10--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s12i0">2013/8/23 00:23:21</td> <td class="M1_exp_item_content"> <table id="i0s12i0" class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18OAI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1808" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1c3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2070" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18OAI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1808" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1c3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2070" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18OAI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1808" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1c3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2070" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="3"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s12i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s12i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s12i1">2013/8/23 00:24:04</td> <td class="M1_exp_item_content"> <table id="i0s12i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="10" class="C4_td_textarea"> <textarea class="C3_textarea" id="i0s12i19"> </textarea></td> </tr> <tr> <td class="C3_td"> </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.84" class="C3_input"></td> <td class="C3_td"><input value="27.9" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="1.76" class="C3_input"></td> <td class="C3_td"><input value="29.3" class="C3_input"></td> </tr> <tr class="C4_tr_C"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="1.68" class="C3_input"></td> <td class="C3_td"><input value="166.3" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i0s12i112" name="#i0s12i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s12i2">2013/8/23 00:24:54</td> <td class="M1_exp_item_content"> <table id="i0s12i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s12i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s14i213.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"><br> <form id="i0s12i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s12i22" form="i0s12i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s12i24"> <input name="id" type="hidden" value="i0s12i2" form="i0s12i24"> <input name="oldgraph" type="hidden" id="i0s12i25" form="i0s12i24" value="t1i0s14i213.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"> <input id="i0s12i26" type="submit" value="submit" form="i0s12i24" class="C1_button2" disabled=""> <div id="i0s12i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s12i21" class="C1_text">WRONG AND USE THE PLASMID TO DO THE DIGESTION</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s12i3">2013/8/23 00:25:28</td> <td class="M1_exp_item_content"><textarea id="i0s12i3" class="C0" rows="1">FAILD</textarea></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18O-AI2M19" id="sb0s13"> <button class="M1_exp_step_name_button"><input value="18O-AI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/23 00:26:59--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i0">2013/8/23 00:27:17</td> <td class="M1_exp_item_content"> <table id="i0s13i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="0" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s13i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s13i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i1">2013/8/23 00:28:07</td> <td class="M1_exp_item_content"> <table class="C5" id="i0s13i1"> <tbody><tr> <th colspan="3" id="i0s13i129" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s13i10"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i0s13i130"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i0s13i11" name="#i0s13i1" value="18O (A)"> <input type="button" class="C5_canvas_button2" id="i0s13i12" name="#i0s13i1" value="AI2M19 (B)"> <input type="button" class="C5_canvas_button3" id="i0s13i13" name="#i0s13i1" value="18O (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="30" class="C3_input"></td> <td class="C3_td"><input value="0" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="50" class="C3_input"></td> <td class="C3_td"><input value="80" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i0s13i126" value="0"> <input type="hidden" id="i0s13i127" value="1"> <input type="hidden" id="i0s13i128" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i2">2013/8/23 00:29:20</td> <td class="M1_exp_item_content"> <table id="i0s13i2" class="C6"> <tbody><tr> <th class="C3_td_title" colspan="5">Electrophoretogram and recover</th> <td rowspan="10" class="C6_td_textarea"> <textarea class="C3_textarea" id="i0s13i26"> </textarea> </td> </tr> <tr> <th colspan="5" class="C3_td"> Analyse Electrophoretogram </th> </tr> <tr class="mdoelC5Correct0s13i2"> <td class="C3_td"></td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> <th class="C3_td">bp</th> <td class="C3_td" rowspan="4"> <input type="button" value="Correct" class="C6_button" name="0s13i2"> </td> </tr> <tr class="mdoelC5Correct0s13i2"> <td class="C3_td" nowrap="nowrap">(A)</td> <td class="C3_td"><input value="1.54" class="C3_input"></td> <td class="C3_td"><input value="6.4" class="C6_input"></td> <td class="C3_td"><input value="2079" class="C6_input"></td> </tr> <tr class="mdoelC5Correct0s13i2"> <td class="C3_td" nowrap="nowrap">(B)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="4" class="C6_input"></td> <td class="C3_td"><input value="1767" class="C6_input"></td> </tr> <tr class="mdoelC5Correct0s13i2" style="display: none;"> <td class="C3_td" nowrap="nowrap">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="mdoelC5Wrong0s13i2" style="display: none;"> <td class="C3_td" colspan="4" rowspan="3"> Possible reason:<br> 1. The validity or contamination of gel extraction kit and DD water.<br> 2. The validity of enzyme and buffer<br> 3. Incomplete dissolution of agar gel<br> </td> <td class="C3_td" rowspan="4"> <input type="button" value="Wrong" class="C6_button" name="0s13i2"> </td> </tr> <tr class="mdoelC5Wrong0s13i2" style="display: none;"> </tr> <tr class="mdoelC5Wrong0s13i2" style="display: none;"> </tr> <tr class="mdoelC5Wrong0s13i2" style="display: none;"> </tr> </tbody></table> <input type="hidden" id="i0s13i27" value="1"></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i3">2013/8/23 00:29:43</td> <td class="M1_exp_item_content"> <table id="i0s13i3" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s13i30"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s13i326">A (vector) /μL</th> <td class="C3_td"><input value="1.574910516447929" class="C3_input"></td> <td class="C3_td"><input value="2" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s13i327">B (insert) /μL</th> <td class="C3_td"><input value="6.425089483552071" class="C3_input"></td> <td class="C3_td"><input value="4" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="3" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i4">2013/8/24 00:33:05</td> <td class="M1_exp_item_content"> <table id="i0s13i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s13i40" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s15i10180 8.24.jpg"><br> <form id="i0s13i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s13i42" form="i0s13i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s13i44"> <input name="id" type="hidden" value="i0s13i4" form="i0s13i44"> <input name="oldgraph" type="hidden" id="i0s13i45" form="i0s13i44" value="t1i0s15i10180 8.24.jpg"> <input id="i0s13i46" type="submit" value="submit" form="i0s13i44" class="C1_button2" disabled=""> <div id="i0s13i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s13i41" class="C1_text">ALL WRONG</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i5">2013/8/25 00:30:23</td> <td class="M1_exp_item_content"> <table id="i0s13i5" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s13i50"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s13i526">A (vector) /μL</th> <td class="C3_td"><input value="1.574910516447929" class="C3_input"></td> <td class="C3_td"><input value="2" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s13i527">B (insert) /μL</th> <td class="C3_td"><input value="6.425089483552071" class="C3_input"></td> <td class="C3_td"><input value="6" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i6">2013/8/25 00:30:39</td> <td class="M1_exp_item_content"><textarea id="i0s13i6" class="C0" rows="1">TRANSFORMATION</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i7">2013/8/26 00:33:48</td> <td class="M1_exp_item_content"> <table id="i0s13i7" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="V1" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18OAI2M19 (9)" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i0s13i74" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="V2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1808" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1c3" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i0s13i712" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2070" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i8">2013/8/26 00:34:19</td> <td class="M1_exp_item_content"> <table id="i0s13i8" class="C10"> <tbody><tr> <th colspan="3">PCR-Reaction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i0s13i80"> </textarea> </td> </tr> <tr> <th class="C3_td">Reaction /μL</th> <td class="C3_td"><input value="25" class="C3_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th> <td class="C3_td"><input value="2.5" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap">dNTP ( Mixture（2.5 mM each） ) /μL</th> <td class="C3_td"><input value="4" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="V1" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="V2" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td">Template /μL <select id="i0s13i810"> <option value="colony">colony</option> <option value="plasmid">plasmid</option> </select> </th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td">rTaq /μL</th> <td class="C3_td"><input value="0.25" class="C10_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>O /μL</th> <td class="C3_td"><input value="15.25" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i9">2013/8/26 00:31:30</td> <td class="M1_exp_item_content"> <table id="i0s13i9" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s13i90" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s15i9180 8.26 18o-2m19 连接鉴定.jpg"><br> <form id="i0s13i94" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s13i92" form="i0s13i94" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s13i94"> <input name="id" type="hidden" value="i0s13i9" form="i0s13i94"> <input name="oldgraph" type="hidden" id="i0s13i95" form="i0s13i94" value="t1i0s15i9180 8.26 18o-2m19 连接鉴定.jpg"> <input id="i0s13i96" type="submit" value="submit" form="i0s13i94" class="C1_button2" disabled=""> <div id="i0s13i93" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s13i91" class="C1_text">ALL WRONG</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i10">2013/8/28 12:02:00</td> <td class="M1_exp_item_content"> <table id="i0s13i10" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s13i100" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s13i10180 8.28 18o-2m19 连接鉴定.jpg"><br> <form id="i0s13i104" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s13i102" form="i0s13i104" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s13i104"> <input name="id" type="hidden" value="i0s13i10" form="i0s13i104"> <input name="oldgraph" type="hidden" id="i0s13i105" form="i0s13i104" value="t1i0s13i10180 8.28 18o-2m19 连接鉴定.jpg"> <input id="i0s13i106" type="submit" value="submit" form="i0s13i104" class="C1_button2" disabled=""> <div id="i0s13i103" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s13i101" class="C1_text">18O-AI2M19 ALL WRONG</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="AI2M19 4 6 10 14 16 17" id="sb0s14"> <button class="M1_exp_step_name_button"><input value="AI2M19 4 6 10 14 16 17" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/29 11:28:51--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i0">2013/8/29 11:29:30</td> <td class="M1_exp_item_content"> <table id="i0s14i0" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="6" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 (6) 4" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="CAGTGATAGAGATATAATGCACTACTAGAG" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i0s14i04" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="7" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="ATAGGGATGTCAATCTCTATCACTG" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i0s14i012" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i1">2013/8/29 11:30:51</td> <td class="M1_exp_item_content"> <table id="i0s14i1" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="8" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 (6) 6" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="TGATAGAGATACTGAGCAC" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i0s14i14" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="9" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="TGATAGGGATGTCAATCTC" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i0s14i112" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i2">2013/8/29 11:32:21</td> <td class="M1_exp_item_content"> <table id="i0s14i2" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 (6) 10" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="CGTGATAGAGATACTGAGCAC" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i0s14i24" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="9" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="TGATAGGGATGTCAATCTC" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i0s14i212" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i3">2013/8/29 11:34:47</td> <td class="M1_exp_item_content"> <table id="i0s14i3" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="PTET14F" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 (6) 14" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="GAGAAAGAGGCGAAATAC" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i0s14i34" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="PTET11R" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="TAGTAGTGCTCAGTATCTC" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i0s14i312" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i4">2013/8/29 11:41:40</td> <td class="M1_exp_item_content"> <table id="i0s14i4" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="PTET16F" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 (6) 16" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="TACTAGATGCGTAAAGGAG" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i0s14i44" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="PTET16R" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="CTCCTCTTTCTCTAGTAG" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i0s14i412" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i5">2013/8/29 11:42:42</td> <td class="M1_exp_item_content"> <table id="i0s14i5" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="PTET17F" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19 (6) 17" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="AGGATACTAGATGCGTAAAGGAG" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i0s14i54" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="PTET17R" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="TTTCTCCTCTTTCTCTAGTAGTGC" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i0s14i512" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i6">2013/8/29 11:43:26</td> <td class="M1_exp_item_content"> <table id="i0s14i6" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s14i60" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s14i713.8.29 ptet 4 6 10 14 16 17.jpg"><br> <form id="i0s14i64" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s14i62" form="i0s14i64" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s14i64"> <input name="id" type="hidden" value="i0s14i6" form="i0s14i64"> <input name="oldgraph" type="hidden" id="i0s14i65" form="i0s14i64" value="t1i0s14i713.8.29 ptet 4 6 10 14 16 17.jpg"> <input id="i0s14i66" type="submit" value="submit" form="i0s14i64" class="C1_button2" disabled=""> <div id="i0s14i63" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s14i61" class="C1_text">ptet 4 6 10 14 16 17</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i7">2013/8/29 11:44:18</td> <td class="M1_exp_item_content"><textarea id="i0s14i7" class="C0" rows="1">DO THE LIGAION</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i8">2013/8/31 11:44:58</td> <td class="M1_exp_item_content"> <table id="i0s14i8" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s14i80" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s14i92013.08.31CH.jpg"><br> <form id="i0s14i84" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s14i82" form="i0s14i84" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s14i84"> <input name="id" type="hidden" value="i0s14i8" form="i0s14i84"> <input name="oldgraph" type="hidden" id="i0s14i85" form="i0s14i84" value="t1i0s14i92013.08.31CH.jpg"> <input id="i0s14i86" type="submit" value="submit" form="i0s14i84" class="C1_button2" disabled=""> <div id="i0s14i83" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s14i81" class="C1_text">4.1 4.2 4.3 9.1 M 9.2 9.3 9.4 9.5 10.1 10.2 10.3 M 14.1 14.2 16.1 16.2 16.3 17.1 17.2 M 17.2
| + | WCC C W</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18OAI2M19" id="sb0s12"> <button class="M1_exp_step_name_button"><input value="18OAI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/23 00:23:10--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s12i0">2013/8/23 00:23:21</td> <td class="M1_exp_item_content"> <table id="i0s12i0" class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18OAI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1808" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1c3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2070" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18OAI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1808" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1c3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2070" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18OAI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon-rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1808" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1c3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2070" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="3"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s12i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s12i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s12i1">2013/8/23 00:24:04</td> <td class="M1_exp_item_content"> <table id="i0s12i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="10" class="C4_td_textarea"> <textarea class="C3_textarea" id="i0s12i19"> </textarea></td> </tr> <tr> <td class="C3_td"> </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.84" class="C3_input"></td> <td class="C3_td"><input value="27.9" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="1.76" class="C3_input"></td> <td class="C3_td"><input value="29.3" class="C3_input"></td> </tr> <tr class="C4_tr_C"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="1.68" class="C3_input"></td> <td class="C3_td"><input value="166.3" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i0s12i112" name="#i0s12i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s12i2">2013/8/23 00:24:54</td> <td class="M1_exp_item_content"> <table id="i0s12i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s12i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s14i213.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"><br> <form id="i0s12i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s12i22" form="i0s12i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s12i24"> <input name="id" type="hidden" value="i0s12i2" form="i0s12i24"> <input name="oldgraph" type="hidden" id="i0s12i25" form="i0s12i24" value="t1i0s14i213.8.23 18o-ai2m19 1 2 3 M 18A 1 2.jpg"> <input id="i0s12i26" type="submit" value="submit" form="i0s12i24" class="C1_button2" disabled=""> <div id="i0s12i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s12i21" class="C1_text">WRONG AND USE THE PLASMID TO DO THE DIGESTION</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s12i3">2013/8/23 00:25:28</td> <td class="M1_exp_item_content"><textarea id="i0s12i3" class="C0" rows="1">FAILD</textarea></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="18O-AI2M19" id="sb0s13"> <button class="M1_exp_step_name_button"><input value="18O-AI2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/23 00:26:59--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i0">2013/8/23 00:27:17</td> <td class="M1_exp_item_content"> <table id="i0s13i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="2013 P5 18O" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pcon" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="ttgacagctagctcagtcctaggtataatgctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="0" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="AI2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-tetr-tt-ptetr-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="agaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="1767" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i0s13i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i0s13i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i1">2013/8/23 00:28:07</td> <td class="M1_exp_item_content"> <table class="C5" id="i0s13i1"> <tbody><tr> <th colspan="3" id="i0s13i129" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s13i10"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i0s13i130"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i0s13i11" name="#i0s13i1" value="18O (A)"> <input type="button" class="C5_canvas_button2" id="i0s13i12" name="#i0s13i1" value="AI2M19 (B)"> <input type="button" class="C5_canvas_button3" id="i0s13i13" name="#i0s13i1" value="18O (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="30" class="C3_input"></td> <td class="C3_td"><input value="0" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="50" class="C3_input"></td> <td class="C3_td"><input value="80" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i0s13i126" value="0"> <input type="hidden" id="i0s13i127" value="1"> <input type="hidden" id="i0s13i128" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i2">2013/8/23 00:29:20</td> <td class="M1_exp_item_content"> <table id="i0s13i2" class="C6"> <tbody><tr> <th class="C3_td_title" colspan="5">Electrophoretogram and recover</th> <td rowspan="10" class="C6_td_textarea"> <textarea class="C3_textarea" id="i0s13i26"> </textarea> </td> </tr> <tr> <th colspan="5" class="C3_td"> Analyse Electrophoretogram </th> </tr> <tr class="mdoelC5Correct0s13i2"> <td class="C3_td"></td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> <th class="C3_td">bp</th> <td class="C3_td" rowspan="4"> <input type="button" value="Correct" class="C6_button" name="0s13i2"> </td> </tr> <tr class="mdoelC5Correct0s13i2"> <td class="C3_td" nowrap="nowrap">(A)</td> <td class="C3_td"><input value="1.54" class="C3_input"></td> <td class="C3_td"><input value="6.4" class="C6_input"></td> <td class="C3_td"><input value="2079" class="C6_input"></td> </tr> <tr class="mdoelC5Correct0s13i2"> <td class="C3_td" nowrap="nowrap">(B)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="4" class="C6_input"></td> <td class="C3_td"><input value="1767" class="C6_input"></td> </tr> <tr class="mdoelC5Correct0s13i2" style="display: none;"> <td class="C3_td" nowrap="nowrap">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="mdoelC5Wrong0s13i2" style="display: none;"> <td class="C3_td" colspan="4" rowspan="3"> Possible reason:<br> 1. The validity or contamination of gel extraction kit and DD water.<br> 2. The validity of enzyme and buffer<br> 3. Incomplete dissolution of agar gel<br> </td> <td class="C3_td" rowspan="4"> <input type="button" value="Wrong" class="C6_button" name="0s13i2"> </td> </tr> <tr class="mdoelC5Wrong0s13i2" style="display: none;"> </tr> <tr class="mdoelC5Wrong0s13i2" style="display: none;"> </tr> <tr class="mdoelC5Wrong0s13i2" style="display: none;"> </tr> </tbody></table> <input type="hidden" id="i0s13i27" value="1"></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i3">2013/8/23 00:29:43</td> <td class="M1_exp_item_content"> <table id="i0s13i3" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s13i30"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s13i326">A (vector) /μL</th> <td class="C3_td"><input value="1.574910516447929" class="C3_input"></td> <td class="C3_td"><input value="2" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s13i327">B (insert) /μL</th> <td class="C3_td"><input value="6.425089483552071" class="C3_input"></td> <td class="C3_td"><input value="4" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="3" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i4">2013/8/24 00:33:05</td> <td class="M1_exp_item_content"> <table id="i0s13i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s13i40" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s15i10180 8.24.jpg"><br> <form id="i0s13i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s13i42" form="i0s13i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s13i44"> <input name="id" type="hidden" value="i0s13i4" form="i0s13i44"> <input name="oldgraph" type="hidden" id="i0s13i45" form="i0s13i44" value="t1i0s15i10180 8.24.jpg"> <input id="i0s13i46" type="submit" value="submit" form="i0s13i44" class="C1_button2" disabled=""> <div id="i0s13i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s13i41" class="C1_text">ALL WRONG</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s13i5">2013/8/25 00:30:23</td> <td class="M1_exp_item_content"> <table id="i0s13i5" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i0s13i50"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s13i526">A (vector) /μL</th> <td class="C3_td"><input value="1.574910516447929" class="C3_input"></td> <td class="C3_td"><input value="2" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i0s13i527">B (insert) /μL</th> <td class="C3_td"><input value="6.425089483552071" class="C3_input"></td> |
| - | C C C C C C C W C C W W C W W W W W W
| + | |
| - | PBAD LINE0(BL21) LINE1(BL21) LINE2(BL21)
| + | |
| - | C C C</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib0s14i9">2013/9/2 11:52:55</td> <td class="M1_exp_item_content"> <table id="i0s14i9" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i0s14i90" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i0s14i1013.9.2 ptet 4.4 10.4 10.5 10.6 14.3 14.4 14.5 16.3 17.1 17.2.jpg"><br> <form id="i0s14i94" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i0s14i92" form="i0s14i94" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i0s14i94"> <input name="id" type="hidden" value="i0s14i9" form="i0s14i94"> <input name="oldgraph" type="hidden" id="i0s14i95" form="i0s14i94" value="t1i0s14i1013.9.2 ptet 4.4 10.4 10.5 10.6 14.3 14.4 14.5 16.3 17.1 17.2.jpg"> <input id="i0s14i96" type="submit" value="submit" form="i0s14i94" class="C1_button2" disabled=""> <div id="i0s14i93" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i0s14i91" class="C1_text">4.4 10.4 10.5 10.6 14.3 M 14.4 14.5 16.3 17.1 17.2
| + | |
| - | ALL WRONG</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
| - | | + | |
| - | <div id="t1e2">
| + | |
| - | <div class="dataOutput_head">
| + | |
| - | pBAD-2M19
| + | |
| - | </div>
| + | |
| - | <div class="dataOutput">
| + | |
| - | <div class="M1_exp_stepInfo" title="pBAD" id="sb1s0"> <button class="M1_exp_step_name_button"><input value="pBAD" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/3 18:01:27--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i0">2013/8/3 18:01:51</td> <td class="M1_exp_item_content"> <table id="i1s0i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pbad" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pbad" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="130" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2155" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i1s0i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i1s0i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i1">2013/8/3 18:02:03</td> <td class="M1_exp_item_content"> <table id="i1s0i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i1s0i19"> </textarea></td> </tr> <tr> <td class="C3_td"> </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.88" class="C3_input"></td> <td class="C3_td"><input value="19" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i1s0i112" name="#i1s0i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i2">2013/8/3 18:03:36</td> <td class="M1_exp_item_content"> <table class="C5" id="i1s0i2"> <tbody><tr> <th colspan="3" id="i1s0i229" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s0i20"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i1s0i230"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i1s0i21" name="#i1s0i2" value="pbad (A)"> <input type="button" class="C5_canvas_button2" id="i1s0i22" name="#i1s0i2" value=" (B)"> <input type="button" class="C5_canvas_button3" id="i1s0i23" name="#i1s0i2" value="pbad (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="0" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="40" class="C3_input"></td> <td class="C3_td"><input value="80" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i1s0i226" value="0"> <input type="hidden" id="i1s0i227" value="1"> <input type="hidden" id="i1s0i228" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s0i3">2013/8/3 18:03:49</td> <td class="M1_exp_item_content"> <table id="i1s0i3" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s0i30" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s0i313.8.3 牛津样，pBAD.jpg"><br> <form id="i1s0i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s0i32" form="i1s0i34" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s0i34"> <input name="id" type="hidden" value="i1s0i3" form="i1s0i34"> <input name="oldgraph" type="hidden" id="i1s0i35" form="i1s0i34" value="t1i1s0i313.8.3 牛津样，pBAD.jpg"> <input id="i1s0i36" type="submit" value="submit" form="i1s0i34" class="C1_button2" disabled=""> <div id="i1s0i33" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s0i31" class="C1_text">OTHER SAMPLE M(500) PBAD
| + | |
| - | | + | |
| - | WRONG AND DO THIS AGAIN TOMORROW</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="PBAD-2M19" id="sb1s1"> <button class="M1_exp_step_name_button"><input value="PBAD-2M19" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/3 18:04:55--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s1i0">2013/8/3 18:05:49</td> <td class="M1_exp_item_content"> <table id="i1s1i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pbad 1 (5)" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pbad" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="130" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2155" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pbad 2 (5)" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pbad" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="130" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2155" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i1s1i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i1s1i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s1i1">2013/8/3 18:08:04</td> <td class="M1_exp_item_content"> <table id="i1s1i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i1s1i19"> </textarea></td> </tr> <tr> <td class="C3_td"> </td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> </tr> <tr> <td class="C3_td" align="center">(A)</td> <td class="C3_td"><input value="1.85" class="C3_input"></td> <td class="C3_td"><input value="105.0" class="C3_input"></td> </tr> <tr> <td class="C3_td" align="center">(B)</td> <td class="C3_td"><input value="1.86" class="C3_input"></td> <td class="C3_td"><input value="148.6" class="C3_input"></td> </tr> <tr class="C4_tr_C" style="display: none;"> <td class="C3_td" align="center">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" colspan="3"> <button class="C8_button" id="i1s1i112" name="#i1s1i1"> Evaluate </button> </th> </tr> <tr style="display: none;"> <td colspan="3"> <table class="C3"> <tbody><tr> <td class="C3_td">A<sub>260/280</sub>>1.8 and >200 ng/μL</td> <td class="C3_td">great</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td">fine</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td" rowspan="2">A<sub>260/280</sub><1.6 or <80 ng/μL</td> <td class="C3_td" rowspan="2">not good</td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> </tbody></table></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="PBAD-2M19 PBAD IS THE BACKBONE" id="sb1s2"> <button class="M1_exp_step_name_button"><input value="PBAD-2M19 PBAD IS THE BACKBONE" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/3 18:10:12--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s2i0">2013/8/3 18:10:41</td> <td class="M1_exp_item_content"> <table id="i1s2i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pbad" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pbad" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="130" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2155" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="857" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i1s2i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i1s2i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s2i1">2013/8/3 18:11:50</td> <td class="M1_exp_item_content"> <table class="C5" id="i1s2i1"> <tbody><tr> <th colspan="3" id="i1s2i129" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s2i10"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i1s2i130"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i1s2i11" name="#i1s2i1" value="pbad (A)"> <input type="button" class="C5_canvas_button2" id="i1s2i12" name="#i1s2i1" value="2M19 (B)"> <input type="button" class="C5_canvas_button3" id="i1s2i13" name="#i1s2i1" value="pbad (A)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="20" class="C3_input"></td> <td class="C3_td"><input value="62" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="60" class="C3_input"></td> <td class="C3_td"><input value="18" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i1s2i126" value="0"> <input type="hidden" id="i1s2i127" value="1"> <input type="hidden" id="i1s2i128" value="0"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s2i2">2013/8/3 19:45:37</td> <td class="M1_exp_item_content"> <table id="i1s2i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s2i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s2i913.8.4 牛津样，pBAD.jpg"><br> <form id="i1s2i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s2i22" form="i1s2i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s2i24"> <input name="id" type="hidden" value="i1s2i2" form="i1s2i24"> <input name="oldgraph" type="hidden" id="i1s2i25" form="i1s2i24" value="t1i1s2i913.8.4 牛津样，pBAD.jpg"> <input id="i1s2i26" type="submit" value="submit" form="i1s2i24" class="C1_button2" disabled=""> <div id="i1s2i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s2i21" class="C1_text">OTHER SAMPLE M(500) PBAD</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s2i3">2013/8/3 18:13:13</td> <td class="M1_exp_item_content"> <table id="i1s2i3" class="C6"> <tbody><tr> <th class="C3_td_title" colspan="5">Electrophoretogram and recover</th> <td rowspan="10" class="C6_td_textarea"> <textarea class="C3_textarea" id="i1s2i36"> </textarea> </td> </tr> <tr> <th colspan="5" class="C3_td"> Analyse Electrophoretogram </th> </tr> <tr class="mdoelC5Correct1s2i3"> <td class="C3_td"></td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> <th class="C3_td">bp</th> <td class="C3_td" rowspan="4"> <input type="button" value="Correct" class="C6_button" name="1s2i3"> </td> </tr> <tr class="mdoelC5Correct1s2i3"> <td class="C3_td" nowrap="nowrap">(A)</td> <td class="C3_td"><input value="1.79" class="C3_input"></td> <td class="C3_td"><input value="13.8" class="C6_input"></td> <td class="C3_td"><input value="2285" class="C6_input"></td> </tr> <tr class="mdoelC5Correct1s2i3"> <td class="C3_td" nowrap="nowrap">(B)</td> <td class="C3_td"><input value="1.86" class="C3_input"></td> <td class="C3_td"><input value="2.6" class="C6_input"></td> <td class="C3_td"><input value="857" class="C6_input"></td> </tr> <tr class="mdoelC5Correct1s2i3" style="display: none;"> <td class="C3_td" nowrap="nowrap">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="mdoelC5Wrong1s2i3" style="display: none;"> <td class="C3_td" colspan="4" rowspan="3"> Possible reason:<br> 1. The validity or contamination of gel extraction kit and DD water.<br> 2. The validity of enzyme and buffer<br> 3. Incomplete dissolution of agar gel<br> </td> <td class="C3_td" rowspan="4"> <input type="button" value="Wrong" class="C6_button" name="1s2i3"> </td> </tr> <tr class="mdoelC5Wrong1s2i3" style="display: none;"> </tr> <tr class="mdoelC5Wrong1s2i3" style="display: none;"> </tr> <tr class="mdoelC5Wrong1s2i3" style="display: none;"> </tr> </tbody></table> <input type="hidden" id="i1s2i37" value="1"></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s2i4">2013/8/3 18:13:35</td> <td class="M1_exp_item_content"> <table id="i1s2i4" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s2i40"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s2i426">A (vector) /μL</th> <td class="C3_td"><input value="1.1474428306551299" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s2i427">B (insert) /μL</th> <td class="C3_td"><input value="6.85255716934487" class="C3_input"></td> <td class="C3_td"><input value="5" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s2i5">2013/8/3 18:14:18</td> <td class="M1_exp_item_content"><textarea id="i1s2i5" class="C0" rows="1">TRANSFORMATION</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s2i6">2013/8/5 11:54:23</td> <td class="M1_exp_item_content"> <table id="i1s2i6" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s2i60"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s2i626">A (vector) /μL</th> <td class="C3_td"><input value="1.1474428306551299" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s2i627">B (insert) /μL</th> <td class="C3_td"><input value="6.85255716934487" class="C3_input"></td> <td class="C3_td"><input value="7" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s2i7">2013/8/8 12:43:18</td> <td class="M1_exp_item_content"> <table id="i1s2i7" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s2i70" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s2i713.8.8(M 1A 2A M 3A 1B M 2B 3B M).jpg"><br> <form id="i1s2i74" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s2i72" form="i1s2i74" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s2i74"> <input name="id" type="hidden" value="i1s2i7" form="i1s2i74"> <input name="oldgraph" type="hidden" id="i1s2i75" form="i1s2i74" value="t1i1s2i713.8.8(M 1A 2A M 3A 1B M 2B 3B M).jpg"> <input id="i1s2i76" type="submit" value="submit" form="i1s2i74" class="C1_button2" disabled=""> <div id="i1s2i73" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s2i71" class="C1_text">ANALYSE BY PCR
| + | |
| - | 1819(23) pbad2m19 1819(34)1 1819(34)2 1819(35)1 1819(35)2
| + | |
| - | C W C C C C </textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s2i8">2013/8/9 12:49:26</td> <td class="M1_exp_item_content"> <table id="i1s2i8" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s2i80" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s2i813.8.9 PBAD 123456鉴定.jpg"><br> <form id="i1s2i84" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s2i82" form="i1s2i84" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s2i84"> <input name="id" type="hidden" value="i1s2i8" form="i1s2i84"> <input name="oldgraph" type="hidden" id="i1s2i85" form="i1s2i84" value="t1i1s2i813.8.9 PBAD 123456鉴定.jpg"> <input id="i1s2i86" type="submit" value="submit" form="i1s2i84" class="C1_button2" disabled=""> <div id="i1s2i83" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s2i81" class="C1_text">ANALYSE BY PCR
| + | |
| - | PBAD 1 2 3 4 5 6
| + | |
| - | C C C</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="PBAD-2M19 2M19 IS THE BACKBONE" id="sb1s3"> <button class="M1_exp_step_name_button"><input value="PBAD-2M19 2M19 IS THE BACKBONE" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/10 22:17:02--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s3i0">2013/8/10 22:17:31</td> <td class="M1_exp_item_content"> <table id="i1s3i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pbad" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pbad" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="130" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2155" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="857" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i1s3i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i1s3i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s3i1">2013/8/10 22:17:54</td> <td class="M1_exp_item_content"> <table class="C5" id="i1s3i1"> <tbody><tr> <th colspan="3" id="i1s3i129" class="C5_th_C">Extract plasmid</th> <td rowspan="10" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s3i10"> </textarea> </td> </tr> <tr> <th class="C5_canvas_td" colspan="3" id="i1s3i130"> <div class="C5_canvas_matrix"> </div> <div class="C5_TEXT1">EX</div> <div class="C5_TEXT2">SP</div> <input type="button" class="C5_canvas_button1" id="i1s3i11" name="#i1s3i1" value="pbad (A)"> <input type="button" class="C5_canvas_button2" id="i1s3i12" name="#i1s3i1" value="2M19 (B)"> <input type="button" class="C5_canvas_button3" id="i1s3i13" name="#i1s3i1" value="2M19 (B)"> </th> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="100" class="C5_system_input"></th> <th class="C3_td">(A)</th> <th class="C3_td">(B)</th> <th class="C3_td_C5_C" style="display: none;">(C)</th> </tr> <tr> <th class="C3_td">Plasmid</th> <td class="C3_td"><input value="60" class="C3_input"></td> <td class="C3_td"><input value="50" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="62" class="C3_input"></td> </tr> <tr> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td"><input value="10" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="10" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Eco</em>R I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td"><em>Xba</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Spe</em> I /μL</th> <td class="C3_td"><input value="5" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="0" class="C3_input"></td> </tr> <tr> <th class="C3_td"><em>Pst</em> I /μL</th> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td"><input value="0" class="C5_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="5" class="C5_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>0 /μL</th> <td class="C3_td"><input value="20" class="C3_input"></td> <td class="C3_td"><input value="30" class="C3_input"></td> <td class="C3_td_C5_C" style="display: none;"><input value="18" class="C3_input"></td> <input type="hidden" id="i1s3i126" value="0"> <input type="hidden" id="i1s3i127" value="1"> <input type="hidden" id="i1s3i128" value="1"> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s3i2">2013/8/10 22:18:10</td> <td class="M1_exp_item_content"> <table id="i1s3i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s3i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s3i213.8.10 回收pbad 2m19.jpg"><br> <form id="i1s3i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s3i22" form="i1s3i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s3i24"> <input name="id" type="hidden" value="i1s3i2" form="i1s3i24"> <input name="oldgraph" type="hidden" id="i1s3i25" form="i1s3i24" value="t1i1s3i213.8.10 回收pbad 2m19.jpg"> <input id="i1s3i26" type="submit" value="submit" form="i1s3i24" class="C1_button2" disabled=""> <div id="i1s3i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s3i21" class="C1_text">PBAD M1000 2M19</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s3i3">2013/8/10 22:18:46</td> <td class="M1_exp_item_content"> <table id="i1s3i3" class="C6"> <tbody><tr> <th class="C3_td_title" colspan="5">Electrophoretogram and recover</th> <td rowspan="10" class="C6_td_textarea"> <textarea class="C3_textarea" id="i1s3i36"> </textarea> </td> </tr> <tr> <th colspan="5" class="C3_td"> Analyse Electrophoretogram </th> </tr> <tr class="mdoelC5Correct1s3i3"> <td class="C3_td"></td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> <th class="C3_td">bp</th> <td class="C3_td" rowspan="4"> <input type="button" value="Correct" class="C6_button" name="1s3i3"> </td> </tr> <tr class="mdoelC5Correct1s3i3"> <td class="C3_td" nowrap="nowrap">(A)</td> <td class="C3_td"><input value="1.41" class="C3_input"></td> <td class="C3_td"><input value="9.7" class="C6_input"></td> <td class="C3_td"><input value="130" class="C6_input"></td> </tr> <tr class="mdoelC5Correct1s3i3"> <td class="C3_td" nowrap="nowrap">(B)</td> <td class="C3_td"><input value="1.41" class="C3_input"></td> <td class="C3_td"><input value="5.9" class="C6_input"></td> <td class="C3_td"><input value="2936" class="C6_input"></td> </tr> <tr class="mdoelC5Correct1s3i3" style="display: none;"> <td class="C3_td" nowrap="nowrap">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="mdoelC5Wrong1s3i3" style="display: none;"> <td class="C3_td" colspan="4" rowspan="3"> Possible reason:<br> 1. The validity or contamination of gel extraction kit and DD water.<br> 2. The validity of enzyme and buffer<br> 3. Incomplete dissolution of agar gel<br> </td> <td class="C3_td" rowspan="4"> <input type="button" value="Wrong" class="C6_button" name="1s3i3"> </td> </tr> <tr class="mdoelC5Wrong1s3i3" style="display: none;"> </tr> <tr class="mdoelC5Wrong1s3i3" style="display: none;"> </tr> <tr class="mdoelC5Wrong1s3i3" style="display: none;"> </tr> </tbody></table> <input type="hidden" id="i1s3i37" value="1"></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s3i4">2013/8/10 22:19:07</td> <td class="M1_exp_item_content"> <table id="i1s3i4" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s3i40"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s3i426">A (insert) /μL</th> <td class="C3_td"><input value="0.5980467963171129" class="C3_input"></td> <td class="C3_td"><input value="0.75" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s3i427">B (vector) /μL</th> <td class="C3_td"><input value="7.401953203682887" class="C3_input"></td> <td class="C3_td"><input value="7.5" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s3i5">2013/8/10 22:19:36</td> <td class="M1_exp_item_content"> <table id="i1s3i5" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s3i50"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s3i526">A (insert) /μL</th> <td class="C3_td"><input value="0.5980467963171129" class="C3_input"></td> <td class="C3_td"><input value="7.5" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s3i527">B (vector) /μL</th> <td class="C3_td"><input value="7.401953203682887" class="C3_input"></td> <td class="C3_td"><input value="0.75" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s3i6">2013/8/10 22:20:43</td> <td class="M1_exp_item_content"><textarea id="i1s3i6" class="C0" rows="1">TRANSFORMATION</textarea></td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="PBAD2M19 4" id="sb1s4"> <button class="M1_exp_step_name_button"><input value="PBAD2M19 4" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/13 23:24:12--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s4i0">2013/8/13 23:25:39</td> <td class="M1_exp_item_content"> <table id="i1s4i0" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P4" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pBAD2M19 (7)" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="CAAAGTGTGACGCCGTGC" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i1s4i04" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P5" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="136" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="GCCATAGCAAGATAGTCC" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i1s4i012" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s4i1">2013/8/13 23:28:09</td> <td class="M1_exp_item_content"> <table id="i1s4i1" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P6" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pBAD2M19 (7)" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="AGCAAAGTGTGACGCCGTG" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i1s4i14" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P5" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="136" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="GCCATAGCAAGATAGTCC" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i1s4i112" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s4i2">2013/8/13 23:28:51</td> <td class="M1_exp_item_content"> <table id="i1s4i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s4i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s4i213.8.13 pbad2m19(45)(67) 19ai2m19 18ai.jpg"><br> <form id="i1s4i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s4i22" form="i1s4i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s4i24"> <input name="id" type="hidden" value="i1s4i2" form="i1s4i24"> <input name="oldgraph" type="hidden" id="i1s4i25" form="i1s4i24" value="t1i1s4i213.8.13 pbad2m19(45)(67) 19ai2m19 18ai.jpg"> <input id="i1s4i26" type="submit" value="submit" form="i1s4i24" class="C1_button2" disabled=""> <div id="i1s4i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s4i21" class="C1_text">pbad2m19(45)(65) ai2m19 18ai
| + | |
| - | C C C W</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="PBAD2M19 1 AND 2" id="sb1s5"> <button class="M1_exp_step_name_button"><input value="PBAD2M19 1 AND 2" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/18 00:00:42--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s5i0">2013/8/18 00:01:15</td> <td class="M1_exp_item_content"> <table id="i1s5i0" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P1" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pBAD2M19 (7)" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="TATGGCATAGCAAAGTGTG" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i1s5i04" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="136" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="GCAATATAGTCCATAAGATTAGCGG" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i1s5i012" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s5i1">2013/8/18 00:02:21</td> <td class="M1_exp_item_content"> <table id="i1s5i1" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P3" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pBAD2M19 (7)" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="TATGGCATAGCAAAGTGTG" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i1s5i14" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="136" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="GCAATATAGTCCATAAGATTAGCGG" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i1s5i112" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s5i2">2013/8/18 00:02:55</td> <td class="M1_exp_item_content"> <table id="i1s5i2" class="C10"> <tbody><tr> <th colspan="3">PCR-Reaction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i1s5i20"> </textarea> </td> </tr> <tr> <th class="C3_td">Reaction /μL</th> <td class="C3_td"><input value="25" class="C3_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th> <td class="C3_td"><input value="2.5" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap">dNTP ( Mixture（2.5 mM each） ) /μL</th> <td class="C3_td"><input value="4" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="P3" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="P2" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td">Template /μL <select id="i1s5i210"> <option value="colony">colony</option> <option value="plasmid">plasmid</option> </select> </th> <td class="C3_td" nowrap="nowrap"><input value="0.01" class="C10_input"></td> </tr> <tr> <th class="C3_td">rTaq /μL</th> <td class="C3_td"><input value="0.25" class="C10_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>O /μL</th> <td class="C3_td"><input value="16.240000000000002" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s5i3">2013/8/18 00:05:05</td> <td class="M1_exp_item_content"> <table id="i1s5i3" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s5i30" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s5i313.8.16 pbad2m19 lian1 lian2.jpg"><br> <form id="i1s5i34" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s5i32" form="i1s5i34" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s5i34"> <input name="id" type="hidden" value="i1s5i3" form="i1s5i34"> <input name="oldgraph" type="hidden" id="i1s5i35" form="i1s5i34" value="t1i1s5i313.8.16 pbad2m19 lian1 lian2.jpg"> <input id="i1s5i36" type="submit" value="submit" form="i1s5i34" class="C1_button2" disabled=""> <div id="i1s5i33" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s5i31" class="C1_text">line 1 and line2</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s5i4">2013/8/20 00:11:54</td> <td class="M1_exp_item_content"> <table id="i1s5i4" class="C10"> <tbody><tr> <th colspan="3">PCR-Reaction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i1s5i40"> </textarea> </td> </tr> <tr> <th class="C3_td">Reaction /μL</th> <td class="C3_td"><input value="25" class="C3_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th> <td class="C3_td"><input value="2.5" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap">dNTP ( Mixture（2.5 mM each） ) /μL</th> <td class="C3_td"><input value="4" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="V1" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="V2" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td">Template /μL <select id="i1s5i410"> <option value="colony">colony</option> <option value="plasmid">plasmid</option> </select> </th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td">rTaq /μL</th> <td class="C3_td"><input value="0.25" class="C10_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>O /μL</th> <td class="C3_td"><input value="6.489999999999998" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s5i5">2013/8/20 00:10:09</td> <td class="M1_exp_item_content"> <table id="i1s5i5" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s5i50" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s5i613.8.19 18A-AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN(123456).jpg"><br> <form id="i1s5i54" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s5i52" form="i1s5i54" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s5i54"> <input name="id" type="hidden" value="i1s5i5" form="i1s5i54"> <input name="oldgraph" type="hidden" id="i1s5i55" form="i1s5i54" value="t1i1s5i613.8.19 18A-AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN(123456).jpg"> <input id="i1s5i56" type="submit" value="submit" form="i1s5i54" class="C1_button2" disabled=""> <div id="i1s5i53" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s5i51" class="C1_text">18A-AI2M19 M PBAD2M19 LIAN1(123456) PBAD2M19 LIAN(123456)
| + | |
| - | W W W WCCCCC WCWWWC</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s5i6">2013/8/31 11:49:18</td> <td class="M1_exp_item_content"> <table id="i1s5i6" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s5i60" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s5i62013.08.31CH.jpg"><br> <form id="i1s5i64" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s5i62" form="i1s5i64" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s5i64"> <input name="id" type="hidden" value="i1s5i6" form="i1s5i64"> <input name="oldgraph" type="hidden" id="i1s5i65" form="i1s5i64" value="t1i1s5i62013.08.31CH.jpg"> <input id="i1s5i66" type="submit" value="submit" form="i1s5i64" class="C1_button2" disabled=""> <div id="i1s5i63" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s5i61" class="C1_text">4.1 4.2 4.3 9.1 M 9.2 9.3 9.4 9.5 10.1 10.2 10.3 M 14.1 14.2 16.1 16.2 16.3 17.1 17.2 M 17.2
| + | |
| - | C C C C C C C W C C W W C W W W W W W
| + | |
| - | PBAD LINE0(BL21) LINE1(BL21) LINE2(BL21)
| + | |
| - | C C C</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="PBAD2M19 4" id="sb1s6"> <button class="M1_exp_step_name_button"><input value="PBAD2M19 4" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/26 00:35:21--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s6i0">2013/8/26 00:35:54</td> <td class="M1_exp_item_content"> <table id="i1s6i0" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P4" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pBAD2M19 (7)" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="CAAAGTGTGACGCCGTGC" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i1s6i04" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P5" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="136" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="GCCATAGCAAGATAGTCC" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i1s6i012" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s6i1">2013/8/26 00:37:04</td> <td class="M1_exp_item_content"> <table id="i1s6i1" class="C10"> <tbody><tr> <th colspan="3">PCR-Reaction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i1s6i10"> </textarea> </td> </tr> <tr> <th class="C3_td">Reaction /μL</th> <td class="C3_td"><input value="50" class="C3_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th> <td class="C3_td"><input value="5" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap">dNTP ( Mixture（2.5 mM each） ) /μL</th> <td class="C3_td"><input value="4" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="P6" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="P5" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td">Template /μL <select id="i1s6i110"> <option value="colony">colony</option> <option value="plasmid">plasmid</option> </select> </th> <td class="C3_td" nowrap="nowrap"><input value="0.1" class="C10_input"></td> </tr> <tr> <th class="C3_td">rTaq /μL</th> <td class="C3_td"><input value="0.25" class="C10_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>O /μL</th> <td class="C3_td"><input value="38.65" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s6i2">2013/8/26 00:40:19</td> <td class="M1_exp_item_content"> <table id="i1s6i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s6i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s6i513.8.26pbadl4.l5.jpg"><br> <form id="i1s6i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s6i22" form="i1s6i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s6i24"> <input name="id" type="hidden" value="i1s6i2" form="i1s6i24"> <input name="oldgraph" type="hidden" id="i1s6i25" form="i1s6i24" value="t1i1s6i513.8.26pbadl4.l5.jpg"> <input id="i1s6i26" type="submit" value="submit" form="i1s6i24" class="C1_button2" disabled=""> <div id="i1s6i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s6i21" class="C1_text">pbad l4.l5</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s6i3">2013/8/27 00:42:13</td> <td class="M1_exp_item_content"><textarea id="i1s6i3" class="C0" rows="1">TRANSFORMATIOIN</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s6i4">2013/8/27 00:42:54</td> <td class="M1_exp_item_content"> <table id="i1s6i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s6i40" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s6i713 8.29 pbad2m19 line4 (1-4).jpg"><br> <form id="i1s6i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s6i42" form="i1s6i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s6i44"> <input name="id" type="hidden" value="i1s6i4" form="i1s6i44"> <input name="oldgraph" type="hidden" id="i1s6i45" form="i1s6i44" value="t1i1s6i713 8.29 pbad2m19 line4 (1-4).jpg"> <input id="i1s6i46" type="submit" value="submit" form="i1s6i44" class="C1_button2" disabled=""> <div id="i1s6i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s6i41" class="C1_text">choose the photo you want to submit</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s6i5">2013/8/28 11:26:54</td> <td class="M1_exp_item_content"> <table id="i1s6i5" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s6i50" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s6i513.8.28 pbad2m19 line4 5(1-6).jpg"><br> <form id="i1s6i54" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s6i52" form="i1s6i54" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s6i54"> <input name="id" type="hidden" value="i1s6i5" form="i1s6i54"> <input name="oldgraph" type="hidden" id="i1s6i55" form="i1s6i54" value="t1i1s6i513.8.28 pbad2m19 line4 5(1-6).jpg"> <input id="i1s6i56" type="submit" value="submit" form="i1s6i54" class="C1_button2" disabled=""> <div id="i1s6i53" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s6i51" class="C1_text">pbad2m19 line4 5 (1-6)
| + | |
| - | WWWWCC CCCCCC</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s6i6">2013/9/16 12:56:58</td> <td class="M1_exp_item_content"><textarea id="i1s6i6" class="C0" rows="1">THE SEQUNCING SHOWS THE GENE KNOCKOUT IS SUCCESSFUL TRANSLATE IT INTO BL21</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s6i7">2013/9/16 12:58:29</td> <td class="M1_exp_item_content"> <table id="i1s6i7" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s6i70" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s6i813.9.16 pbad2m19 line4 123456.jpg"><br> <form id="i1s6i74" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s6i72" form="i1s6i74" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s6i74"> <input name="id" type="hidden" value="i1s6i7" form="i1s6i74"> <input name="oldgraph" type="hidden" id="i1s6i75" form="i1s6i74" value="t1i1s6i813.9.16 pbad2m19 line4 123456.jpg"> <input id="i1s6i76" type="submit" value="submit" form="i1s6i74" class="C1_button2" disabled=""> <div id="i1s6i73" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s6i71" class="C1_text">PBAD2M19 LINE 4 IN BL21 123456 ALL CORRECT</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="PBAD2M10 5" id="sb1s7"> <button class="M1_exp_step_name_button"><input value="PBAD2M10 5" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/8/26 00:39:17--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s7i0">2013/8/26 00:39:25</td> <td class="M1_exp_item_content"> <table id="i1s7i0" class="C9"> <tbody><tr> <th colspan="6">Template and Primers</th> </tr> <tr> <th class="C3_td" colspan="3"> choose plasmid </th> <th class="C3_td" rowspan="4"> primer1 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P6" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pBAD2M19 (7)" class="C3_input"></td> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="AGCAAAGTGTGACGCCGTG" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> <th class="C3_td"> direction </th> <td class="C3_td"> <select id="i1s7i04" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td" colspan="2"> type </th> <td class="C3_td"><input value="pbad-rbs-gfp-tt" class="C3_input"></td> <th class="C3_td"> position </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> <th class="C3_td" rowspan="4"> primer2 </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="P5" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="136" class="C3_input"></td> <th class="C3_td">sequence</th> <td class="C3_td"><input value="GCCATAGCAAGATAGTCC" class="C3_input"></td> </tr> <tr> <th class="C3_td" rowspan="2"> backbone </th> <th class="C3_td">name</th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> <th class="C3_td">direction</th> <td class="C3_td"> <select id="i1s7i012" class="C3_input"> <option value="left">left</option> <option value="right">right</option> </select> </td> </tr> <tr> <th class="C3_td">length</th> <td class="C3_td"><input value="2079" class="C3_input"></td> <th class="C3_td">position</th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s7i1">2013/8/26 00:40:04</td> <td class="M1_exp_item_content"> <table id="i1s7i1" class="C10"> <tbody><tr> <th colspan="3">PCR-Reaction</th> <td rowspan="9" class="C4_td_textarea"> <textarea class="C3_textarea" id="i1s7i10"> </textarea> </td> </tr> <tr> <th class="C3_td">Reaction /μL</th> <td class="C3_td"><input value="50" class="C3_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="10" class="C10_system_input"></th> <td class="C3_td"><input value="5" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap">dNTP ( Mixture（2.5 mM each） ) /μL</th> <td class="C3_td"><input value="4" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="P6" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td" nowrap="nowrap"><input value="P5" class="C10_system_input"></th> <td class="C3_td" nowrap="nowrap"><input value="1" class="C10_input"></td> </tr> <tr> <th class="C3_td">Template /μL <select id="i1s7i110"> <option value="colony">colony</option> <option value="plasmid">plasmid</option> </select> </th> <td class="C3_td" nowrap="nowrap"><input value="0.1" class="C10_input"></td> </tr> <tr> <th class="C3_td">rTaq /μL</th> <td class="C3_td"><input value="0.25" class="C10_input"></td> </tr> <tr> <th class="C3_td">H<sub>2</sub>O /μL</th> <td class="C3_td"><input value="38.65" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s7i2">2013/8/26 00:40:51</td> <td class="M1_exp_item_content"> <table id="i1s7i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s7i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s7i213.8.26pbadl4.l5.jpg"><br> <form id="i1s7i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s7i22" form="i1s7i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s7i24"> <input name="id" type="hidden" value="i1s7i2" form="i1s7i24"> <input name="oldgraph" type="hidden" id="i1s7i25" form="i1s7i24" value="t1i1s7i213.8.26pbadl4.l5.jpg"> <input id="i1s7i26" type="submit" value="submit" form="i1s7i24" class="C1_button2" disabled=""> <div id="i1s7i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s7i21" class="C1_text">pbad l4.l5</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s7i3">2013/8/27 00:42:25</td> <td class="M1_exp_item_content"><textarea id="i1s7i3" class="C0" rows="1">TRANSFORMATION</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s7i4">2013/8/28 11:27:57</td> <td class="M1_exp_item_content"> <table id="i1s7i4" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s7i40" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s7i413.8.28 pbad2m19 line4 5(1-6).jpg"><br> <form id="i1s7i44" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s7i42" form="i1s7i44" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s7i44"> <input name="id" type="hidden" value="i1s7i4" form="i1s7i44"> <input name="oldgraph" type="hidden" id="i1s7i45" form="i1s7i44" value="t1i1s7i413.8.28 pbad2m19 line4 5(1-6).jpg"> <input id="i1s7i46" type="submit" value="submit" form="i1s7i44" class="C1_button2" disabled=""> <div id="i1s7i43" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s7i41" class="C1_text">pbad2m19 line4 5 (1-6)
| + | |
| - | WWWWCC CCCCCC</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table> <div class="M1_exp_stepInfo" title="LINE 0,1,2 IN C3" id="sb1s8"> <button class="M1_exp_step_name_button"><input value="LINE 0,1,2 IN C3" class="M1_exp_step_name"></button> <span class="M1_exp_step_time">2013/9/6 12:40:40--Ruosang</span> </div> <table class="M1_exp_stepBoard"> <tbody><tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s8i0">2013/9/6 12:41:24</td> <td class="M1_exp_item_content"> <table id="i1s8i0" class="C8"> <tbody><tr> <th colspan="2" class="C8_td_title"> Start to Ligate Plasmids </th> </tr> <tr> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (A) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="C3" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="BACKBONE" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> <td> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (B) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="pBAD2M19" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="pbad-rbs-gfp-tt" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="993" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="psb1a2" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="2079" class="C3_input"></td> </tr> </tbody></table> </td> <td style="display: none;"> <table class="C8"> <tbody><tr> <th colspan="3" class="C8_td_title"> choose plasmid (C) </th> </tr> <tr> <th rowspan="2" class="C3_td"> plasmid </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> location </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> type </th> <td class="C3_td" colspan="2"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> part-only </th> <th class="C3_td"> sequence </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th rowspan="2" class="C3_td"> backbone </th> <th class="C3_td"> name </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr> <th class="C3_td"> length </th> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table> </td> </tr> <tr> <td class="C3_td" colspan="2"> <input type="button" class="C8_button" value="if you need another plasmid (C) , double click here" name="#i1s8i0"> <input type="button" class="C8_button" value="if you don't need the plasmid (C) , double click here" name="#i1s8i0" style="display: none;"> </td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s8i1">2013/9/6 12:42:40</td> <td class="M1_exp_item_content"><textarea id="i1s8i1" class="C0" rows="1">DIGESTE BY 5uL EcoRI AND PstI</textarea></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s8i2">2013/9/6 12:43:17</td> <td class="M1_exp_item_content"> <table id="i1s8i2" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s8i20" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s8i313.9.6 C3 LINE0 LINE1 LINE2.jpg"><br> <form id="i1s8i24" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s8i22" form="i1s8i24" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s8i24"> <input name="id" type="hidden" value="i1s8i2" form="i1s8i24"> <input name="oldgraph" type="hidden" id="i1s8i25" form="i1s8i24" value="t1i1s8i313.9.6 C3 LINE0 LINE1 LINE2.jpg"> <input id="i1s8i26" type="submit" value="submit" form="i1s8i24" class="C1_button2" disabled=""> <div id="i1s8i23" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s8i21" class="C1_text">C3 LINE0 LINE1 LINE2
| + | |
| - | C W C W</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s8i3">2013/9/6 12:44:27</td> <td class="M1_exp_item_content"> <table id="i1s8i3" class="C6"> <tbody><tr> <th class="C3_td_title" colspan="5">Electrophoretogram and recover</th> <td rowspan="10" class="C6_td_textarea"> <textarea class="C3_textarea" id="i1s8i36"> </textarea> </td> </tr> <tr> <th colspan="5" class="C3_td"> Analyse Electrophoretogram </th> </tr> <tr class="mdoelC5Correct1s8i3"> <td class="C3_td"></td> <th class="C3_td">A<sub>260/280</sub></th> <th class="C3_td">ng/μL</th> <th class="C3_td">bp</th> <td class="C3_td" rowspan="4"> <input type="button" value="Correct" class="C6_button" name="1s8i3"> </td> </tr> <tr class="mdoelC5Correct1s8i3"> <td class="C3_td" nowrap="nowrap">(A)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="38.1" class="C6_input"></td> <td class="C3_td"><input value="2070" class="C6_input"></td> </tr> <tr class="mdoelC5Correct1s8i3"> <td class="C3_td" nowrap="nowrap">(B)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="8.4" class="C6_input"></td> <td class="C3_td"><input value="993" class="C6_input"></td> </tr> <tr class="mdoelC5Correct1s8i3" style="display: none;"> <td class="C3_td" nowrap="nowrap">(C)</td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="mdoelC5Wrong1s8i3" style="display: none;"> <td class="C3_td" colspan="4" rowspan="3"> Possible reason:<br> 1. The validity or contamination of gel extraction kit and DD water.<br> 2. The validity of enzyme and buffer<br> 3. Incomplete dissolution of agar gel<br> </td> <td class="C3_td" rowspan="4"> <input type="button" value="Wrong" class="C6_button" name="1s8i3"> </td> </tr> <tr class="mdoelC5Wrong1s8i3" style="display: none;"> </tr> <tr class="mdoelC5Wrong1s8i3" style="display: none;"> </tr> <tr class="mdoelC5Wrong1s8i3" style="display: none;"> </tr> </tbody></table> <input type="hidden" id="i1s8i37" value="1"></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s8i4">2013/9/6 12:44:44</td> <td class="M1_exp_item_content"> <table id="i1s8i4" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s8i40"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s8i426">A (vector) /μL</th> <td class="C3_td"><input value="1.0627720362233637" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s8i427">B (insert) /μL</th> <td class="C3_td"><input value="6.937227963776635" class="C3_input"></td> <td class="C3_td"><input value="7" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s8i5">2013/9/7 12:48:34</td> <td class="M1_exp_item_content"> <table id="i1s8i5" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s8i50" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s8i613.9.7 pbad2m19 line0 line2.jpg"><br> <form id="i1s8i54" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s8i52" form="i1s8i54" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s8i54"> <input name="id" type="hidden" value="i1s8i5" form="i1s8i54"> <input name="oldgraph" type="hidden" id="i1s8i55" form="i1s8i54" value="t1i1s8i613.9.7 pbad2m19 line0 line2.jpg"> <input id="i1s8i56" type="submit" value="submit" form="i1s8i54" class="C1_button2" disabled=""> <div id="i1s8i53" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s8i51" class="C1_text">0 AND 2</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s8i6">2013/9/7 12:49:01</td> <td class="M1_exp_item_content"> <table id="i1s8i6" class="C7"> <tbody><tr> <th class="C3_td_title" colspan="3"> Ligation </th> <td rowspan="8" class="C3_td_textarea"> <textarea class="C3_textarea" id="i1s8i60"> </textarea> </td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C7_blur_1"></th> <td colspan="2" class="C3_td" nowrap="nowrap"><input value="3" class="C7_blur_1"></td> </tr> <tr class="C7_tr_AB"> <td class="C3_td"></td> <th class="C3_td">Recommend</th> <th class="C3_td">Actual use</th> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s8i626">A (vector) /μL</th> <td class="C3_td"><input value="1.0627720362233637" class="C3_input"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" id="i1s8i627">B (insert) /μL</th> <td class="C3_td"><input value="6.937227963776635" class="C3_input"></td> <td class="C3_td"><input value="7" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_AB"> <th class="C3_td">ligase /μL</th> <td class="C3_td"><input value="1" class="C7_blur_2"></td> <td class="C3_td"><input value="1" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <th class="C3_td" nowrap="nowrap"><input value="25" class="C5_system_input"></th> <td class="C3_td">Recommend</td> <td class="C3_td">Actual use</td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">A /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">B /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">C /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td" nowrap="nowrap"><input value="10" class="C5_system_input"></td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">ligase /μL</td> <td class="C3_td"><input value="1" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> <tr class="C7_tr_ABC" style="display: none;"> <td class="C3_td">H<sub>2</sub>O /μL</td> <td class="C3_td"><input value="20" class="C7_input_C"></td> <td class="C3_td"><input value="" class="C3_input"></td> </tr> </tbody></table></td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s8i7">2013/9/7 12:49:19</td> <td class="M1_exp_item_content"> <table id="i1s8i7" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s8i70" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s8i813.9.8 c3-line1(1) c3-line1(2) 2M19D5 2M19D5 500.jpg"><br> <form id="i1s8i74" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s8i72" form="i1s8i74" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s8i74"> <input name="id" type="hidden" value="i1s8i7" form="i1s8i74"> <input name="oldgraph" type="hidden" id="i1s8i75" form="i1s8i74" value="t1i1s8i813.9.8 c3-line1(1) c3-line1(2) 2M19D5 2M19D5 500.jpg"> <input id="i1s8i76" type="submit" value="submit" form="i1s8i74" class="C1_button2" disabled=""> <div id="i1s8i73" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s8i71" class="C1_text">LINE 1 IN C3 OTHER SAMPLE M
| + | |
| - | C C</textarea> <br> </td> </tr> </tbody></table> </td> </tr> <tr class="M1_exp_stepItem"> <td class="M1_exp_item_time" id="ib1s8i8">2013/9/9 12:50:45</td> <td class="M1_exp_item_content"> <table id="i1s8i8" class="C1"> <tbody><tr> <td class="C1_td"> <img id="i1s8i80" class="C1_graph" src="http://trysomething-block.stor.sinaapp.com/t1i1s8i913.9.9 pbad2m19-c3 line0 1 2 ,line2 1 2.jpg"><br> <form id="i1s8i84" target="M7" action="img.php" method="post" enctype="multipart/form-data"></form> <input name="img" type="file" id="i1s8i82" form="i1s8i84" class="C1_button1" required="required" disabled=""> <input name="pname" type="hidden" value="t1" form="i1s8i84"> <input name="id" type="hidden" value="i1s8i8" form="i1s8i84"> <input name="oldgraph" type="hidden" id="i1s8i85" form="i1s8i84" value="t1i1s8i913.9.9 pbad2m19-c3 line0 1 2 ,line2 1 2.jpg"> <input id="i1s8i86" type="submit" value="submit" form="i1s8i84" class="C1_button2" disabled=""> <div id="i1s8i83" class="M1_exp_img"></div> </td> <td class="C1_td_text"> <textarea id="i1s8i81" class="C1_text">ANALYSE LINE 0 1，2 M LINE 2 1，2</textarea> <br> </td> </tr> </tbody></table> </td> </tr> </tbody></table>
| + | |
| - | </div>
| + | |
| - | </div>
| + | |
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| - | }
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| - | </script>
| + | |
| - | </body>
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| - | </html>
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