Team:XMU Software/html

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Revision as of 09:11, 24 October 2013 by YuezhenChen (Talk | contribs)

18A-AI-2M19
2013/7/29 09:15:49--Ruosang
2013/7/29 09:16:24
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/7/30 09:18:19
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/7/30 09:19:34


2013/7/30 09:21:18
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/7/30 09:21:31


2013/7/30 09:22:23
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/7/30 09:23:12
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/7/31 09:23:55
2013/8/1 09:24:26--Ruosang
2013/8/1 09:24:42
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/1 09:25:15
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/1 09:25:39


2013/8/5 21:44:19--Ruosang
2013/8/5 21:45:25
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/5 21:45:55


2013/8/6 21:46:26
2013/8/7 21:50:17
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/7 21:50:56
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/7 21:52:12
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/7 21:52:52
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/8 21:54:37


2013/8/9 22:05:07


2013/8/9 22:13:49
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/9 22:14:05
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/16 23:58:07
2013/8/7 21:46:54--Ruosang
2013/8/7 21:47:35
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/7 21:48:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/7 21:48:47


2013/8/9 22:02:31


2013/8/9 22:15:05
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/9 22:15:18
Cultivation
Plasmid (A )
Strain
antibiotics
Medium
Volume
Temperature
Speed
Plasmid (B )
Strain
antibiotics
Medium
Volume
Temperature
Speed
Plasmid (C )
Strain
antibiotics
Medium
Volume
Temperature
Speed
2013/8/10 22:16:21
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/16 23:58:45
2013/8/8 21:56:45--Ruosang
2013/8/8 21:57:12
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/8 22:08:20


2013/8/9 22:09:20--Ruosang
2013/8/9 22:09:38
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/9 22:10:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/9 22:11:10
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/9 22:11:57


2013/8/12 22:22:06--Ruosang
2013/8/12 22:22:24
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/12 22:22:43
Cultivation
Plasmid (A )
Strain
antibiotics
Medium
Volume
Temperature
Speed
Plasmid (B )
Strain
antibiotics
Medium
Volume
Temperature
Speed
Plasmid (C )
Strain
antibiotics
Medium
Volume
Temperature
Speed
2013/8/13 23:21:13
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/13 23:21:47


2013/8/13 23:25:17
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/13 23:36:12


2013/8/15 23:41:26--Ruosang
2013/8/15 23:41:42
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/15 23:44:36
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/15 23:43:57
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/15 23:47:13
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/15 23:52:24
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/15 23:54:43


2013/8/15 23:55:52
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/8/15 23:56:29
Ligation
Recommend Actual use
A (insert) /μL
B (vector) /μL
ligase /μL
2013/8/16 23:59:03
2013/8/17 23:59:37


2013/8/19 00:07:27


2013/8/19 00:09:02
Ligation
Recommend Actual use
A (insert) /μL
B (vector) /μL
ligase /μL
2013/8/20 00:13:32


2013/8/20 00:14:31--Ruosang
2013/8/20 00:14:48
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/20 00:15:21


2013/8/20 00:16:34


2013/8/20 00:16:50
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/21 00:17:26--Ruosang
2013/8/21 00:17:45
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/21 00:18:04
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/22 00:18:33--Ruosang
2013/8/22 00:18:50
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/22 00:19:07


2013/8/22 00:20:05
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/22 00:20:33
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/23 00:21:17--Ruosang
2013/8/23 00:21:30
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/23 00:21:48


2013/8/23 00:23:10--Ruosang
2013/8/23 00:23:21
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/23 00:24:04
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
(C)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/23 00:24:54


2013/8/23 00:25:28
2013/8/23 00:26:59--Ruosang
2013/8/23 00:27:17
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/23 00:28:07
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/23 00:29:20
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/8/23 00:29:43
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/24 00:33:05


2013/8/25 00:30:23
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/25 00:30:39
2013/8/26 00:33:48
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/26 00:34:19
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/26 00:31:30


2013/8/28 12:02:00


2013/8/29 11:28:51--Ruosang
2013/8/29 11:29:30
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:30:51
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:32:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:34:47
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:41:40
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:42:42
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/29 11:43:26


2013/8/29 11:44:18
2013/8/31 11:44:58


2013/9/2 11:52:55


pBAD-2M19
2013/8/3 18:01:27--Ruosang
2013/8/3 18:01:51
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/3 18:02:03
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/3 18:03:36
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/3 18:03:49


2013/8/3 18:04:55--Ruosang
2013/8/3 18:05:49
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/3 18:08:04
Plasmid Extraction
      A260/280 ng/μL
(A)
(B)
A260/280>1.8 and >200 ng/μL great
fine
A260/280<1.6 or <80 ng/μL not good
2013/8/3 18:10:12--Ruosang
2013/8/3 18:10:41
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/3 18:11:50
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/3 19:45:37


2013/8/3 18:13:13
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/8/3 18:13:35
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/3 18:14:18
2013/8/5 11:54:23
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/8/8 12:43:18


2013/8/9 12:49:26


2013/8/10 22:17:02--Ruosang
2013/8/10 22:17:31
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/8/10 22:17:54
Extract plasmid
EX
SP
(A) (B)
Plasmid
EcoR I /μL
Xba I /μL
Spe I /μL
Pst I /μL
H20 /μL
2013/8/10 22:18:10


2013/8/10 22:18:46
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/8/10 22:19:07
Ligation
Recommend Actual use
A (insert) /μL
B (vector) /μL
ligase /μL
2013/8/10 22:19:36
Ligation
Recommend Actual use
A (insert) /μL
B (vector) /μL
ligase /μL
2013/8/10 22:20:43
2013/8/13 23:24:12--Ruosang
2013/8/13 23:25:39
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/13 23:28:09
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/13 23:28:51


2013/8/18 00:00:42--Ruosang
2013/8/18 00:01:15
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/18 00:02:21
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/18 00:02:55
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/18 00:05:05


2013/8/20 00:11:54
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/20 00:10:09


2013/8/31 11:49:18


2013/8/26 00:35:21--Ruosang
2013/8/26 00:35:54
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/26 00:37:04
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/26 00:40:19


2013/8/27 00:42:13
2013/8/27 00:42:54


2013/8/28 11:26:54


2013/9/16 12:56:58
2013/9/16 12:58:29


2013/8/26 00:39:17--Ruosang
2013/8/26 00:39:25
Template and Primers
choose plasmid primer1 name
plasmid name sequence
location direction
type position
part-only sequence primer2 name
length sequence
backbone name direction
length position
2013/8/26 00:40:04
PCR-Reaction
Reaction /μL
dNTP ( Mixture(2.5 mM each) ) /μL
Template /μL
rTaq /μL
H2O /μL
2013/8/26 00:40:51


2013/8/27 00:42:25
2013/8/28 11:27:57


2013/9/6 12:40:40--Ruosang
2013/9/6 12:41:24
Start to Ligate Plasmids
choose plasmid (A)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (B)
plasmid name
location
type
part-only sequence
length
backbone name
length
choose plasmid (C)
plasmid name
location
type
part-only sequence
length
backbone name
length
2013/9/6 12:42:40
2013/9/6 12:43:17


2013/9/6 12:44:27
Electrophoretogram and recover
Analyse Electrophoretogram
A260/280 ng/μL bp
(A)
(B)
2013/9/6 12:44:44
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/9/7 12:48:34


2013/9/7 12:49:01
Ligation
Recommend Actual use
A (vector) /μL
B (insert) /μL
ligase /μL
2013/9/7 12:49:19


2013/9/9 12:50:45