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Team:Yale/Project Validate
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== Aims for the Project == | == Aims for the Project == | ||
| - | <style="color:red;"> | + | #<style="color:red;">'''Engineer strains of ''E. coli'' to validate PLA synthesis'''</style> <br> |
#'''Develop bioassay to screen PLA production'''<br> | #'''Develop bioassay to screen PLA production'''<br> | ||
#'''Apply MAGE to optimize PLA production, guided by FBA'''<br> | #'''Apply MAGE to optimize PLA production, guided by FBA'''<br> | ||
Revision as of 01:07, 22 August 2013
Aims for the Project
- <style="color:red;">Engineer strains of E. coli to validate PLA synthesis</style>
- Develop bioassay to screen PLA production
- Apply MAGE to optimize PLA production, guided by FBA
- Introduce type 1 secretion system to export and extract PLA
- Make a bioplastic
Engineer strains of E. coli to validate PLA synthesis
| This was our plan in order to validate PLA synthesis. In order to save money we ordered each of the two heterologous genes in four pieces from IDT giving us 8 GBlocks. |
