From 2013.igem.org
Aug 31
No name
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | -- | --
|
| 3 | RBS-lysis2-(9) | EcoRI & SpeI
|
| 4
|
| 5 | -- | --
|
| 6 | pSB1C3(1) | EcoRI & SpeI
|
| 7
|
| 8 | -- | --
|
| 9 | pSB1C3(1) | XbaI&PstI
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | tRNA-Spinach | EcoRI & SpeI
|
| 3
|
| 4 | -- | --
|
| 5 | pT181 attenuator-(2) | EcoRI & SpeI
|
| 6
|
| 7 | pT181 attenuator-(2) | XbaI & PstI
|
| 8
|
| 9 | -- | --
|
| 10 | pT181 antisense-(2) | EcoRI & SpeI
|
| 11
|
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme
|
| 1 | 100bp ladder | --
|
| 2 | pT181 antisense | XbaRI & PstI
|
| 3
|
| 4 | -- | --
|
| 5 | tetR aptamer 12_1R-(1) | EcoRI & SpeI
|
| 6
|
| Name | concentration[µg/mL] | 260/280 | 260/230
|
| Pcon-RBS-GFP-DT (EcoRI&SpeI) | 7.1 | 1.88 | 0.07
|
| Pcon-RBS-luxR-DT (EcoRI&XbaI) | 15.3 | 1.93 | 0.70
|
| Plux (SpeI&PstI) | 4.5 | 1.79 | 0.31
|
| RBS-GFP-DT (XbaI&PstI) | 6.0 | 2.16 | 0.03
|
| RBS-tetR-DT (XbaI&PstI) | 7.6 | 1.91 | 0.38
|
"
