Template:Kyoto/Notebook/Sep 8

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Contents

Sep 8

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
1100bp ladder
2RBS-lysis1-DTX+P
3RBS-lysis1-DTX+P
5RBS-lysis3-DTX+P
6RBS-lysis3-DTX+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
RBS-lysis1-DT(X+P) 5.31.980.06
RBS-lysis3-DT(X+P) 5.31.980.06
LaneDNAEnzyme
1100bp ladder
3Pcon-pT181 atteE+S
4Pcon-pT181 atteE+S
6Spinach-DTX+P
7Spinach-DTX+P
9RBS-lacZα-DTX+P
10RBS-lacZα-DTX+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
Pcon-pT181 atte(E+S) 5.21.820.36
Spinach-DT(X+P) 8.01.870.25
RBS-lacZα-DT(X+P) 10.31.710.54
LaneDNAEnzyme
11kbp ladder
2Ptet-pT181-antiE+P
3Ptet-pT181-antiE+P
5pSB4K5E+S
6pSB4K5E+S

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
Ptet-pT181-anti(E+P) 30.61.841.16
pSB4K5(E+S) 21.81.870.98
LaneDNAEnzyme
11kbp ladder
2Pcon-pT181 atteS+P
3Pcon-pT181 atteS+P
5PlacS+P
6PlacS+P
8pSB1C3E+S
9pSB1C3E+S
11pSB1C3X+P
12pSB1C3X+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
Pcon-pT181 atte(S+P) 14.51.911.09
Plac(S+P) 11.61.741.19
pSB1C3(E+S) 5.71.740.34
pSB1C3(X+P) 5.62.000.37

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
Pcon-pT181 anti243.01.901.81
Pcon-Spinach-DT253.71.571.51

Restriction Enzyme Digestion

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts(°°) µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts(°°) µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

Transformation

じっけんしゃ

NameSampleCompetent CellsTotalPlate
いでんしなんちゃらµLごにょごにょµLほにゃららµLこーせーぶっしつ
いかどうよう

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
(°Д°)
LaneSampleEnzyme1Enzyme2
(°Д°)
LaneSampleEnzyme1Enzyme2
(°Д°)

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
(>ω・)b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
(´ω`)
(ΦωΦ^)
LaneDNAEnzyme
(>ω・)b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
(´ω`)
(ΦωΦ^)
LaneDNAEnzyme
(>ω・)b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
(´ω`)
(ΦωΦ^)
LaneDNAEnzyme
(>ω・)b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
(´ω`)
(ΦωΦ^)
LaneDNAEnzyme
(>ω・)b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
(´ω`)
(ΦωΦ^)

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL
stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
 °C °C °C °C--
(◎Д◎)    

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
(>ω・)b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
(´ω`)
(ΦωΦ^)
LaneDNAEnzyme
(>ω・)b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
(´ω`)
(ΦωΦ^)