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{

ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1> Name of participants: Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Todays work:</h2> Mutagenesis 338.pSB1C3-meffBlue Plasmidprepp: 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 301. pEL3S17-J23110-B0034-Thioredoxin Digest: 147.pSB1C3-B0034-Thioredoxin Ligate: 147.pSB1C3-B0034-Thioredoxin+CP1→ → 346.pSB1C3-CP1-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP8→ →347.pSB1C3-CP8-B0034-Thioredoxin 147.pSB1C3-B0034-Thioredoxin+CP41→ →348.pSB1C3-CP41-B0034-Thioredoxin Transformation: 1.pSB1C3-Red (competens test) 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 208.pSB1A3-B0034-Thioredoxin-ZCD-His 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 285.pSB1C3.J23110-B0034-aeBlue 286.pSB1C3.J23110-B0034-amilCP 287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ 336.pSB1C3-spisPink 337pSB1C3-scOrange 346.pSB1C3-CP1-B0034-Thioredoxin 347.pSB1C3-CP8-B0034-Thioredoxin 348.pSB1C3-CP41-B0034-Thioredoxin 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple Re-streak: 284. pSB1C3-B0034-aeBlue Sequenceprep: 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) </div>'

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else if(id == 'd2013826')

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{

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ds = '<div id="dairy-text"><h1>Monday 2013-08-26</h1> Name of participants: Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M <h2>Ongoing constructs:</h2> 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin <h2>Todays work</h2> Streak from frozen stock: 68. pSB3K3-CP1-B0034-BFP 69. pSB3K3-CP8-B0034-BFP 73. pSB3K3-CP41-B0034-BFP 139. pSB3K3-J23101-B0034-BFP PCR: Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures +2° and +4°C) Slovenian 4CL with His-primers Gel electrophoresis: Erythromycin-cassette PCR-product from Lb2.1 pLUL631 Erythromycin-cassette PCR-product from Lb2.2 pLUL631 Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R Assembly & transformation: 349 Mutagenesis 338.pSB1C3-meffBlue Re streak 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple 343. pSB1C3-J23106-B0034-asCP Plasmidprepp: 102.pSB1C3-CrtE 272.pSB1K3-B0034-Thioredoxin-ZCD-His (272F.pSB1K3-B0034-Thioredoxin-ZCD-His) 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin O/N screen 284. pSB1C3-B0034-aeBlue Screening: 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 346.pSB1C3-CP1-B0034-Thioredoxin 347.pSB1C3-CP8-B0034-Thioredoxin 348. pSB1C3-CP41-B0034-Thioredoxin O/N: 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (annan ligering än ovan, den som screenades) Re-streak 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (den som screenades) Sekvensprep: 102.pSB1C3-CrtE 272.pSB1K3-B0034-Thioredoxin-ZCD-His (272F.pSB1K3-B0034-Thioredoxin-ZCD-His) 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 143. pEL3S17-CP8-B0034-UGTCs2-6xHis 179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 265. J23110-B0034-Exp-Linker-Miraculin-His 155. pSB1C3-j23110-RBS-meffRed 156. pSB1C3-j23106-RBS-meffRed 157. pSB1C3-j23110-RBS-spisPink 158. pSB1C3-j23106-RBS-spisPink 247. pSB1C3-J23110-RBS-tsPurple 248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees) 249. pSB1C3-J23110-RBS-scOrange 250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees) 251. pSB1C3-J23110-RBS-fwYellow 252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees) 339. pSB1C3-gfasPurple 340. pSB1C3-amajCP(amajLime) 341. pSB1C3-tsPurple 342. pSB1C3-fwYellow Ligation: 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP1→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP8→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP41→ → 346.pSB1K3-CP1-B0034-Thioredoxin 6. pSB1K3-Red + 20.1.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 → →215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→ → 303.pSB1K3-J23101-B0034-CrtZ 127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→ → 223.pSB3K3-CP8-B0034-UGTCs2-His <h2>Results:</h2> Gel electrophoresis: Erythromycin-cassette PCR-product: Good bands! → proceed with PCR-cleanup and assembly Erythromycin-cassette PCR-product: --”-- Lb9. faecalis JH2-2 pAMbeta1-ori: One of the two had a good band → proceed 77. pSB4C15-red: Unspecific binding → redo PCR with +2° and +4° annealing temp. 269 showed correct bands. 247 and 248 showed credible bands, but a mistake in the assembly was detected (resistance on source of insert is the same as target backbone). These samples were discarded and a new ligation with the backbone pSB1K3 was made. <h2>Characterization:</h2> Western blot sample preparation: 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS <h2>Other experiments</h2> Preparing MRS-agar Preparing low salt LB-agar</div>'

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}

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else if(id == 'd2013827')

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{

+

ds = '<div id="dairy-text"><h1>Tuesday 2013-08-27</h1> Name of participants: Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus <h2>Ongoing constructs:</h2> 102.pSB1C3-CrtE 108.pEL3S17-J23106-B0034-IspA 109.pEL3S17-K206000-B0034-IspA 110.pEL3S17-K206001-B0034-IspA 140.pEL3S17-J23110-B0034-UGTCs2-6xHis 141.pEL3S17-J23101-B0034-UGTCs2-6xHis 142.pEL3S17-CP1-B0034-UGTCs2-6xHis 143.pEL3S17-CP8-B0034-UGTCs2-6xHis 147.pSB1C3-B0034-Thioredoxin 163.pEL3S17-ZCD-His-B0034-UGTCs2-His 177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His 178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His 179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His 180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His 181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His 182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His 183.pEL3A15-B0034-CrtZ 184.pSB1C3-B0034-CrtZ (201. E.Coli. Nissle - CrtY-CrtI) 202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268 203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 208. pSB1A3-B0034-Thioredoxin-ZCD-His 213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 220.pSB3K3-J23110-B0034-UGTCs2-His 221.pSB3T5-J23101-B0034-UGTCs2-His 222.pSB3K3-CP1-B0034-UGTCs2-His 223.pSB3K3-CP8-B0034-UGTCs2-His 264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His 265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His 266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His 269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His 272. pSB1K3-B0034-Thioredoxin-ZCD-His 299. pEL3S17-J23101-B0034-Thioredoxin 300. pEL3S17-J23106-B0034-Thioredoxin 301. pEL3S17-J23110-B0034-Thioredoxin 303. pSB1A3-j23101-B0034-CrtZ 304. pSB1A3-j23106-B0034-CrtZ 305.pSB1K3-J23110-CrtZ 308.pSB1C3-Miraculin 309.pSB1C3-B0034-Miraculin 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His 261. pSB3K3-J23110-B0034-His-4CL 333. pSB3K3-J23110-HIS-STS 334. pSB3K3-CP1-His-STS 335. pSB3K3-J23101-HIs-STS 349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS <h2>Todays work</h2> O/N: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 Transformation: Chromoproteins into pSB3K3 215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 223.pSB3K3-CP8-B0034-UGTCs2-His 303.pSB1K3-j23101-B0034-CrtZ 346.pSB1K3-CP1-B0034-Thioredoxin 347.pSB1K3-CP8-B0034-Thioredoxin 348.pSB1K3-CP41-B0034-Thioredoxin Gel electrophoresis: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures +2° and +4°C) Screening PCR: 351.1 pSB1C3-pemp-mut1 351.2 pSB1C3-pemp-mut1 377.pSB1A3-CP8-B0034-UGTCs2-His 378.pSB1A3-J23110-B0034-UGTCs2-His 379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His 380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His 381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His 382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His Mutagenesis 338.pSB1C3-meffBlue Plasmidprepp: 182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ Sekvensprep: 182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His 269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His 303.pSB1K3-j23101-B0034-CrtZ 284. pSB1C3-B0034-aeBlue Re-streak 285. pSB1C3-J23110-B0034-aeBlue 286. pSB1C3-J23110-B0034-amilCP 287. pSB1C3-J23110-B0034-asCP 336.pSB1C3-spisPink 337.pSB1C3-scOrange 159. pSB1C3-J23110-RBS-gfasPurple 160. pSB1C3-J23110-RBS-gfasPurple 343. pSB1C3-J23106-B0034-asCP PCR: 77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R* *(with annealing temperatures Tm1 and +5°C (with touchdown)) Streaking of plasmid free stock on test plates: - e. coli (10. and 60.3) onto erythromycin LB-plates: 5 µg/ml 10 µg/ml 20 µg/ml 50 µg/ml - lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations: 50 µg/ml 100 µg/ml <h2>Results</h2> Gel electrophoresis: 77. pSB4C15-red +2°C annealing temperature: Strange band at 1000 bp 77. pSB4C15-red +4°C annealing temperature: Strange band at 1000 bp <h2>Other experiments</h2> Preparing test plates, to determine minimum antibiotic concentration: Lb-erythromycin: 5 µg/ml 10 µg/ml 20 µg/ml 50 µg/ml MRS-zeocin: 50 µg/ml 100 µg/ml</div>'

}

else if(id == 'd2013919')

@@ Line 527: / Line 527: @@
   {
 	ds = '<div id="dairy-text"><h1>Sunday 2013-08-25</h1><br><b>Name of participants:</b> Alexander, Pontus, Sabri, Malin, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Todays work:</h2><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Plasmidprepp:</b><br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265.pSB3K3.J23110-B0034-Exp-Linker-Miraculin-His<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180. pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>301. pEL3S17-J23110-B0034-Thioredoxin<br><br><b>Digest:</b><br>147.pSB1C3-B0034-Thioredoxin<br><br><b>Ligate:</b><br>147.pSB1C3-B0034-Thioredoxin+CP1→<br>→ 346.pSB1C3-CP1-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP8→<br>→347.pSB1C3-CP8-B0034-Thioredoxin<br>147.pSB1C3-B0034-Thioredoxin+CP41→<br>→348.pSB1C3-CP41-B0034-Thioredoxin<br><br><b>Transformation:</b><br>1.pSB1C3-Red (competens test)<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>208.pSB1A3-B0034-Thioredoxin-ZCD-His<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>285.pSB1C3.J23110-B0034-aeBlue<br>286.pSB1C3.J23110-B0034-amilCP<br>287.pSB1C3-J23110-B0034-asCP303.pSB1K3-j23101-B0034-CrtZ<br>336.pSB1C3-spisPink<br>337pSB1C3-scOrange<br>346.pSB1C3-CP1-B0034-Thioredoxin<br>347.pSB1C3-CP8-B0034-Thioredoxin<br>348.pSB1C3-CP41-B0034-Thioredoxin<br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br><br><b>Re-streak:</b><br>284. pSB1C3-B0034-aeBlue<br><br><b>Sequenceprep:</b><br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br></div>'
+ }
+      else if(id == 'd2013826')
+ {
+	ds = '<div id="dairy-text"><h1>Monday 2013-08-26</h1><br><b>Name of participants: </b>Alona Nyberg, Mikael Strandgren, Anders Edlund, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M<br><br><h2>Ongoing constructs:</h2><br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><h2>Todays work</h2><br><b>Streak from frozen stock:</b><br>68. pSB3K3-CP1-B0034-BFP<br>69. pSB3K3-CP8-B0034-BFP<br>73. pSB3K3-CP41-B0034-BFP<br>139. pSB3K3-J23101-B0034-BFP<br><br><b>PCR:</b><br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures +2° and +4°C)</i><br>Slovenian 4CL with His-primers<br><br><b>Gel electrophoresis:</b><br>Erythromycin-cassette PCR-product from Lb2.1 pLUL631<br>Erythromycin-cassette PCR-product from Lb2.2 pLUL631<br>Lb9. faecalis JH2-2 pAMbeta1-ori with pAM-d-BII_F and pAM-s-X1_R<br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R<br><br><b>Assembly & transformation:</b><br>349<br><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Re streak</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br>343. pSB1C3-J23106-B0034-asCP<br><br><b>Plasmidprepp:</b><br>102.pSB1C3-CrtE<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>(272F.pSB1K3-B0034-Thioredoxin-ZCD-His)<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br><br><b>O/N screen</b><br>284. pSB1C3-B0034-aeBlue<br><br><b>Screening:</b><br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>346.pSB1C3-CP1-B0034-Thioredoxin<br>347.pSB1C3-CP8-B0034-Thioredoxin<br>348. pSB1C3-CP41-B0034-Thioredoxin<br><br><b>O/N:</b><br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (annan ligering än ovan, den som screenades)<br><br><b>Re-streak</b><br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His (den som screenades)<br><br><b>Sekvensprep:</b><br>102.pSB1C3-CrtE<br>272.pSB1K3-B0034-Thioredoxin-ZCD-His<br>(272F.pSB1K3-B0034-Thioredoxin-ZCD-His)<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>143. pEL3S17-CP8-B0034-UGTCs2-6xHis<br>179. pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>265. J23110-B0034-Exp-Linker-Miraculin-His<br>155. pSB1C3-j23110-RBS-meffRed<br>156. pSB1C3-j23106-RBS-meffRed<br>157. pSB1C3-j23110-RBS-spisPink<br>158. pSB1C3-j23106-RBS-spisPink<br>247. pSB1C3-J23110-RBS-tsPurple<br>248. pSB1C3-J23106-RBS-tsPurple (grow 30 degrees)<br>249. pSB1C3-J23110-RBS-scOrange<br>250. pSB1C3-J23106-RBS-scOrange (grow 30 degrees)<br>251. pSB1C3-J23110-RBS-fwYellow<br>252. pSB1C3-J23106-RBS-fwYellow (grow 30 degrees)<br>339. pSB1C3-gfasPurple<br>340. pSB1C3-amajCP(amajLime)<br>341. pSB1C3-tsPurple<br>342. pSB1C3-fwYellow<br><br><b>Ligation:</b><br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP1→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP8→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 147.pSB1C3-B0034-Thioredoxin +CP41→<br>→ 346.pSB1K3-CP1-B0034-Thioredoxin<br>6. pSB1K3-Red + 20.1.pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 → <br>→215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>184.pSB1C3-B0034-CrtZ+6.pSB1K3-Red+103.pSB1K3-J23101→<br>→ 303.pSB1K3-J23101-B0034-CrtZ<br>127.pSB1A3-B0034-UGTCs2-His+7.pSB3K3-Red+CP8→<br>→ 223.pSB3K3-CP8-B0034-UGTCs2-His<br><br><h2>Results:</h2><br><b>Gel electrophoresis: </b><br>Erythromycin-cassette PCR-product: Good bands! → proceed with PCR-cleanup and assembly<br>Erythromycin-cassette PCR-product:  			--”--<br>Lb9. faecalis JH2-2 pAMbeta1-ori: One of the two had a good band → proceed<br>77. pSB4C15-red: Unspecific binding → redo PCR with +2° and +4° annealing temp.<br>269 showed correct bands. 247 and 248 showed credible bands, but a mistake in the assembly was detected (resistance on source of insert is the same as target backbone). These samples were discarded and a new ligation with the backbone pSB1K3 was made.<br><br><h2>Characterization:</h2><br><b>Western blot sample preparation:</b><br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br><br><h2>Other experiments</h2><br><b>Preparing MRS-agar</b><br><br><b>Preparing low salt LB-agar</b></div>'
+ }
+      else if(id == 'd2013827')
+ {
+	ds = '<div id="dairy-text"><h1>Tuesday  2013-08-27</h1><br><b>Name of participants: </b>Viktor Törnblom, Viktor Blomkvist, Alona Nyberg, Mikael Strandgren, Marcus H, Karl H, Hampus.E, Lovisa P, Emil M, Alexander, Pontus, Sabri, Magnus<br><br><h2>Ongoing constructs:</h2><br>102.pSB1C3-CrtE<br>108.pEL3S17-J23106-B0034-IspA<br>109.pEL3S17-K206000-B0034-IspA<br>110.pEL3S17-K206001-B0034-IspA<br>140.pEL3S17-J23110-B0034-UGTCs2-6xHis<br>141.pEL3S17-J23101-B0034-UGTCs2-6xHis<br>142.pEL3S17-CP1-B0034-UGTCs2-6xHis<br>143.pEL3S17-CP8-B0034-UGTCs2-6xHis<br>147.pSB1C3-B0034-Thioredoxin<br>163.pEL3S17-ZCD-His-B0034-UGTCs2-His<br>177.pEL3S17-J23101-B0034-Exp-Linker-Miraculin-His<br>178.pEL3S17-J23110-B0034-Exp-Linker-Miraculin-His<br>179.pEL3S17-CP1-B0034-Exp-Linker-Miraculin-His<br>180.pEL3S17-CP8-B0034-Exp-Linker-Miraculin-His<br>181.pSBLb4C15-CP1-B0034-Exp-Linker-Miraculin-His<br>182.pSBLb4C15-CP8-B0034-Exp-Linker-Miraculin-His<br>183.pEL3A15-B0034-CrtZ<br>184.pSB1C3-B0034-CrtZ<br>(201. E.Coli. Nissle - CrtY-CrtI)<br>202.pSB4C5-CrtE-B0034-Blues-CrtB-B0034-CrtI-Linker-Zif268<br>203.pSB1C3-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>208. pSB1A3-B0034-Thioredoxin-ZCD-His<br>213.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>214.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>216.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>217.pSB3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>218.pEL3K16-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>220.pSB3K3-J23110-B0034-UGTCs2-His<br>221.pSB3T5-J23101-B0034-UGTCs2-His<br>222.pSB3K3-CP1-B0034-UGTCs2-His<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>264.pSB3K3-J23101-B0034-Exp-Linker-Miraculin-His<br>265.psB3K3-J23110-B0034-Exp-Linker-Miraculin-His<br>266.psB3K3-CP1-B0034-Exp-Linker-Miraculin-His<br>269.psB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>272. pSB1K3-B0034-Thioredoxin-ZCD-His<br>299. pEL3S17-J23101-B0034-Thioredoxin<br>300. pEL3S17-J23106-B0034-Thioredoxin<br>301. pEL3S17-J23110-B0034-Thioredoxin<br>303. pSB1A3-j23101-B0034-CrtZ<br>304. pSB1A3-j23106-B0034-CrtZ<br>305.pSB1K3-J23110-CrtZ<br>308.pSB1C3-Miraculin<br>309.pSB1C3-B0034-Miraculin<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br>261. pSB3K3-J23110-B0034-His-4CL<br>333. pSB3K3-J23110-HIS-STS<br>334. pSB3K3-CP1-His-STS<br>335. pSB3K3-J23101-HIs-STS<br>349. pSB3K3-B0034-TAL(mut)-B0034-4CL-B0034-STS<br><br><h2>Todays work</h2><br><b>O/N:</b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br><br><b>Transformation:</b><br>Chromoproteins into pSB3K3<br>215.pSB1K3-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>223.pSB3K3-CP8-B0034-UGTCs2-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>346.pSB1K3-CP1-B0034-Thioredoxin<br>347.pSB1K3-CP8-B0034-Thioredoxin<br>348.pSB1K3-CP41-B0034-Thioredoxin<br><br><b>Gel electrophoresis:</b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures +2° and +4°C)</i><br><br><b>Screening PCR: </b><br>351.1 pSB1C3-pemp-mut1<br>351.2 pSB1C3-pemp-mut1<br>377.pSB1A3-CP8-B0034-UGTCs2-His<br>378.pSB1A3-J23110-B0034-UGTCs2-His<br>379.pSB1K3-CP8-B0034-Thioredoxin-ZCD-His<br>380.pSB1K3-J23110-B0034-Thioredoxin-ZCD-His<br>381.pSB1C3-CP8-B0034-Exp-Linker-Miraculin-Linker-His<br>382.pSB1C3-J23110-B0034-Exp-Linker-Miraculin-Linker-His<br><br><b>Mutagenesis</b><br>338.pSB1C3-meffBlue<br><br><b>Plasmidprepp:</b><br>182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br><br><b>Sekvensprep:</b><br>182.pSBLb4C5-CP8-B0034-Exp-Linker-Miraculin-His<br>269.pSB3K3-CP8-B0034-Exp-Linker-Miraculin-His<br>303.pSB1K3-j23101-B0034-CrtZ<br>284. pSB1C3-B0034-aeBlue<br><br><b>Re-streak</b><br>285. pSB1C3-J23110-B0034-aeBlue<br>286. pSB1C3-J23110-B0034-amilCP<br>287. pSB1C3-J23110-B0034-asCP<br>336.pSB1C3-spisPink<br>337.pSB1C3-scOrange<br>159. pSB1C3-J23110-RBS-gfasPurple<br>160. pSB1C3-J23110-RBS-gfasPurple<br>343. pSB1C3-J23106-B0034-asCP<br><br><b>PCR:</b><br>77. pSB4C15-red with pEL-C1_F and pEL-d-BII_R*<br><i>*(with annealing temperatures Tm1 and +5°C (with touchdown))</i><br><br><b>Streaking of plasmid free stock on test plates:</b><br><i>- e. coli (10. and 60.3) onto erythromycin LB-plates:</i><br>5 µg/ml<br>10 µg/ml<br>20 µg/ml<br>50 µg/ml<br><i>- lactobacillus (Lb4., Lb11. and Lb12.) onto Zeocin (bleomycin) MRS-plates of varying concentrations:</i><br>50 µg/ml<br>100 µg/ml<br><br><h2>Results</h2><br><b>Gel electrophoresis:</b><br>77. pSB4C15-red +2°C annealing temperature: Strange band at 1000 bp<br>77. pSB4C15-red +4°C annealing temperature: Strange band at 1000 bp<br><br><h2>Other experiments</h2><br><b>Preparing test plates, to determine minimum antibiotic concentration:</b><br>Lb-erythromycin:<br>5 µg/ml<br>10 µg/ml<br>20 µg/ml<br>50 µg/ml<br><br><b>MRS-zeocin:</b><br>50 µg/ml<br>100 µg/ml</div>'
   }
   else if(id == 'd2013919')

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