To learn about this mechanism, click here2.

From 2013.igem.org

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== Model highlight 2:How capsule concentration influences Bee.coli survival rate ==
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{{:Team:NYMU-Taipei/Header}}
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We do this model in order to know whether capsules ingested by bees could be effective enough. That is, whether capsules could be digested by bees’ digestive juice, Bee.coli in capsules is able to proliferate, and finally, Bee. coli could reach the effective concentration (Bee.coli/bee) to defend Nosema infection. (Figure 1.)
 
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The data of capsule digestion rate and Bee.coli survival rate is from experiment, while Bee.coli proliferation rate is assumed according to several papers [1] and confirmed dividing output by input.
 
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The input is capsule concentration and the function contains the transfer of capsule digestion, survival and proliferation of Bee.coli, which then generates the output of Bee.coli/bee.
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The synergistic effect of Lux and CI to pLux/CI is complicated and is not characterized in quantity yet. To simplify the pLux/CI hybrid promoter’s behavior in our circuit regulation model and at the same time enables wet lab to characterize it experimentally, we a module showing the relationships between CI negative regulation and Lux-AHL complex positive regulation.
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Last but not least, we retrieve the standard concentration of capsule-contained sugar water which will be fed to be colony via the formula below:
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In our regulation process of our project we mainly face three scene: CI present while Lux absent, CI and Lux both absent, and CI absent while Lux present.
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Promoter strength shown in the picture are subtracted to form differences in promoter strength OA, AB, BC. OA represent the leaky effect of CI regulation; AB represent the promoter strength regulated by CI negatively; BC represent the promoter strength regulated by AHL-Lux complex positively. Then they’re quantified by Hill effect expression [[(to learn more about the ODE regarding pLux/CI promoter, click here)]]
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This simplification combines wet lab and dry lab’s effort that we can describe the promoter’s effect in ODEs for the model in dry lab while wet lab can verify it and provide parameters by doing experiment.
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<div lang="latex" class="equation">
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[capsule  concentration]={[Bee.coli  per  bee]}\times {proliferation  rate}\frac {1}{{digestion  rate  (of    capsule)}\times {survival  rate  (of  Bee.coli})}
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</div>
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</html>
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[[Image:NYMU_ highlight 2.png|center]]
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[[Image:NYMU_hill property.png|center]]
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Figure1: model of capsule concentration and Bee.coli survival rate|center
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This picture gives a concept of our model for pLux/CI behavior. The promoter strength of pLux/CI when CI present while Lux absent, CI and Lux both absent, and CI absent while Lux present are measured respectively.
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{{:Team:NYMU-Taipei/Footer}}

Latest revision as of 03:59, 28 October 2013

National Yang Ming University



The synergistic effect of Lux and CI to pLux/CI is complicated and is not characterized in quantity yet. To simplify the pLux/CI hybrid promoter’s behavior in our circuit regulation model and at the same time enables wet lab to characterize it experimentally, we a module showing the relationships between CI negative regulation and Lux-AHL complex positive regulation.

In our regulation process of our project we mainly face three scene: CI present while Lux absent, CI and Lux both absent, and CI absent while Lux present. Promoter strength shown in the picture are subtracted to form differences in promoter strength OA, AB, BC. OA represent the leaky effect of CI regulation; AB represent the promoter strength regulated by CI negatively; BC represent the promoter strength regulated by AHL-Lux complex positively. Then they’re quantified by Hill effect expression (to learn more about the ODE regarding pLux/CI promoter, click here)

This simplification combines wet lab and dry lab’s effort that we can describe the promoter’s effect in ODEs for the model in dry lab while wet lab can verify it and provide parameters by doing experiment.

NYMU hill property.png

This picture gives a concept of our model for pLux/CI behavior. The promoter strength of pLux/CI when CI present while Lux absent, CI and Lux both absent, and CI absent while Lux present are measured respectively.


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