UIUC Illinois/Safety/Database

From 2013.igem.org

(Difference between revisions)
(Created page with "==Safety Database== ===EtBr=== ===Solid Disposal=== ===Liquid Disposal=== ===General decontamination=== ===Electrophoresis machine=== ===Proper Bunsen burner technique=== ===Appr...")
Line 1: Line 1:
==Safety Database==
==Safety Database==
-
===EtBr===
+
==EtBr==
-
===Solid Disposal===
+
[www.purdue.edu/rem/hmm/ethidbr.htm]
-
===Liquid Disposal===
+
*Ethidium bromide(EtBR) is a known toxin and mutagen. Its use should be limited and it should be handled with EXTREME CARE.
-
===General decontamination===
+
*Only use EtBr in designated areas clearly marked “EtBr contamination” to avoid contaminating other lab areas and equipment.
-
===Electrophoresis machine===
+
*Any PPE that has been contaminated with EtBr must be disposed of in an EtBr container
-
===Proper Bunsen burner technique===
+
**Latex gloves should not be worn when working with EtBr. Nitrile gloves are sufficient when minimal exposure time is expected. For longer periods of time, use multiple layers or chemically-resistant gloves.
-
===Appropriate PPE===
+
**Wash hands thoroughly and immediately after disposing of gloves
 +
*If accidental exposure occurs:
 +
**Eyes- rinse with water for 15 minutes
 +
**Skin- wash the contaminated area with soap and water
 +
**Clothing- remove and place in sealed bag
 +
**ALWAYS seek medical attention immediately after an accident has occurred
 +
*Methyl Blue alternative- not nearly as hazardous, but may not be as effective as EtBr
 +
==Solid Disposal==
 +
==Liquid Disposal==
 +
==General decontamination==
 +
==Electrophoresis machine==
 +
==Proper Bunsen burner technique==
 +
==Appropriate PPE==
 +
 
 +
 
 +
 
 +
<!-- Resource: http://www.mediawiki.org/wiki/Help:Contents Honestly I just follow this to a T and it is quite easy to create a wikipedia page so go ahead and edit as you like :) -->

Revision as of 13:28, 13 August 2013

Contents

Safety Database

EtBr

[www.purdue.edu/rem/hmm/ethidbr.htm]

  • Ethidium bromide(EtBR) is a known toxin and mutagen. Its use should be limited and it should be handled with EXTREME CARE.
  • Only use EtBr in designated areas clearly marked “EtBr contamination” to avoid contaminating other lab areas and equipment.
  • Any PPE that has been contaminated with EtBr must be disposed of in an EtBr container
    • Latex gloves should not be worn when working with EtBr. Nitrile gloves are sufficient when minimal exposure time is expected. For longer periods of time, use multiple layers or chemically-resistant gloves.
    • Wash hands thoroughly and immediately after disposing of gloves
  • If accidental exposure occurs:
    • Eyes- rinse with water for 15 minutes
    • Skin- wash the contaminated area with soap and water
    • Clothing- remove and place in sealed bag
    • ALWAYS seek medical attention immediately after an accident has occurred
  • Methyl Blue alternative- not nearly as hazardous, but may not be as effective as EtBr

Solid Disposal

Liquid Disposal

General decontamination

Electrophoresis machine

Proper Bunsen burner technique

Appropriate PPE