User:Earthnut

From 2013.igem.org

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<html><table border="1" cellspacing="0" width="100%" !important><tr><td colspan="200" width="20%"><strong>BioBrick: <partinfo>BBa_E0040</partinfo></strong></td><td colspan="800" width="80%"><strong>Automatically determined parameters using the <a href="https://2013.igem.org/Team:TU-Munich/Results/Software">BioBrick-AutoAnnotator</a> version 1.0</strong></td></tr><tr><td colspan="100" width="10%"><strong>RFC standard:</strong></td><td colspan="600" width="60%">RFC 10 BioBrick, nothing was added</td><td colspan="300" width="30%">ORF from 1 to 714 (excluding stop-codon)</td></tr><tr><td colspan="1000"><strong>Nucleotide sequence:</strong> (underlined part encodes the protein, italic parts were added)<br><span style="font-family:'Courier New', Arial;">&nbsp;<u>ATGCGTAAA&nbsp;...&nbsp;CTATACAAA</u>TAATAA</span></td></tr><tr><td colspan="1000"><strong>Amino acid sequence:</strong><br><span style="font-family:'Courier New', Arial;">&nbsp;&nbsp;1&nbsp;MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFF<br>101&nbsp;KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY<br>201&nbsp;LSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK<br></span></td></tr><tr><td colspan="1000"><strong>Amino acid composition:</strong></td></tr><tr><td colspan="100" width="10.0%">A (Ala)</td><td colspan=" 99" width=" 9.9%">9 (3.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">C (Cys)</td><td colspan=" 99" width=" 9.9%">2 (0.8%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">H (His)</td><td colspan=" 99" width=" 9.9%">10 (4.2%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">M (Met)</td><td colspan=" 99" width=" 9.9%">6 (2.5%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">T (Thr)</td><td colspan="100" width="10.0%">15 (6.3%)</td></tr><tr><td colspan="100" width="10.0%">R (Arg)</td><td colspan=" 99" width=" 9.9%">7 (2.9%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Q (Gln)</td><td colspan=" 99" width=" 9.9%">8 (3.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">I (Ile)</td><td colspan=" 99" width=" 9.9%">12 (5.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">F (Phe)</td><td colspan=" 99" width=" 9.9%">13 (5.5%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">W (Trp)</td><td colspan="100" width="10.0%">1 (0.4%)</td></tr><tr><td colspan="100" width="10.0%">N (Asn)</td><td colspan=" 99" width=" 9.9%">13 (5.5%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">E (Glu)</td><td colspan=" 99" width=" 9.9%">16 (6.7%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">L (Leu)</td><td colspan=" 99" width=" 9.9%">19 (8.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">P (Pro)</td><td colspan=" 99" width=" 9.9%">10 (4.2%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Y (Tyr)</td><td colspan="100" width="10.0%">11 (4.6%)</td></tr><tr><td colspan="100" width="10.0%">D (Asp)</td><td colspan=" 99" width=" 9.9%">18 (7.6%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">G (Gly)</td><td colspan=" 99" width=" 9.9%">23 (9.7%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">K (Lys)</td><td colspan=" 99" width=" 9.9%">20 (8.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">S (Ser)</td><td colspan=" 99" width=" 9.9%">8 (3.4%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">V (Val)</td><td colspan="100" width="10.0%">17 (7.1%)</td></tr><tr><td colspan="100" width="10.0%"><strong>Amino acid counting:</strong></td><td colspan="200" width="20.0%">Total number of amino acids (aa):</td><td colspan="100" width="10.0%">238</td><td colspan="200" width="20.0%">Positively charged aa (Arg + Lys):</td><td colspan="100" width="10.0%">27</td><td colspan="200" width="20.0%">Negatively charged aa (Asp + Glu):</td><td colspan="100" width="10.0%">34</td></tr><tr><td colspan="100" width="10.0%"><strong>Biochemical parameters:</strong></td><td colspan="200" width="20.0%">Molecular weight [Da]:</td><td colspan="100" width="10.0%">26909.4</td><td colspan="200" width="20.0%">Theoretical pI:</td><td colspan="100" width="10.0%">5.8</td><td colspan="200" width="20.0%">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td colspan="100" width="10.0%">22015 (all Cys as cystine)<br>21890 (all Cys reduced)</td></tr><tr><td colspan="100" width="10.0%"><strong>Estimated half-life [h]:</strong></td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">>10</td><td colspan="200" width="20.0%"><i>S. cervisiae:</i></td><td colspan="100" width="10.0%">>20</td><td colspan="200" width="20.0%">Mammals:</td><td colspan="100" width="10.0%">30</td></tr><tr><td colspan="100" width="10.0%"><strong>Codon usage (CAI):</strong></td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">0.72</td><td colspan="200" width="20.0%"><i>S. cervisiae:</i></td><td colspan="100" width="10.0%">0.75</td><td colspan="200" width="20.0%">Mammals:</td><td colspan="100" width="10.0%">0.68</td></tr><tr><td colspan="1000" width="100.0%"> The BioBrick-AutoAnnotator was created by <a href="https://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For information please read the <a href="https://2013.igem.org/Team:TU-Munich/Results/Software">description</a>.</td></tr></table><br></html>

Latest revision as of 18:17, 27 July 2013

BioBrick: BBa_E0040Automatically determined parameters using the BioBrick-AutoAnnotator version 1.0
RFC standard:RFC 10 BioBrick, nothing was addedORF from 1 to 714 (excluding stop-codon)
Nucleotide sequence: (underlined part encodes the protein, italic parts were added)
 ATGCGTAAA ... CTATACAAATAATAA
Amino acid sequence:
  1 MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFF
101 KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY
201 LSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK
Amino acid composition:
A (Ala)9 (3.8%)C (Cys)2 (0.8%)H (His)10 (4.2%)M (Met)6 (2.5%)T (Thr)15 (6.3%)
R (Arg)7 (2.9%)Q (Gln)8 (3.4%)I (Ile)12 (5.0%)F (Phe)13 (5.5%)W (Trp)1 (0.4%)
N (Asn)13 (5.5%)E (Glu)16 (6.7%)L (Leu)19 (8.0%)P (Pro)10 (4.2%)Y (Tyr)11 (4.6%)
D (Asp)18 (7.6%)G (Gly)23 (9.7%)K (Lys)20 (8.4%)S (Ser)8 (3.4%)V (Val)17 (7.1%)
Amino acid counting:Total number of amino acids (aa):238Positively charged aa (Arg + Lys):27Negatively charged aa (Asp + Glu):34
Biochemical parameters:Molecular weight [Da]:26909.4Theoretical pI:5.8Extinction coefficient at 280 nm [M-1 cm-1]:22015 (all Cys as cystine)
21890 (all Cys reduced)
Estimated half-life [h]:E. coli:>10S. cervisiae:>20Mammals:30
Codon usage (CAI):E. coli:0.72S. cervisiae:0.75Mammals:0.68
The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For information please read the description.