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From 2013.igem.org

Gel electrophoresis

• Two different sets of gel were run due to the different fragment size of the PCR product.
• Smaller fragments were run on a 2% agarose gel whilst larger fragments were run on a 1% gel.

DNA extraction from gel

• Extracting previously run DNA from gel using Thermo Scientific GeneJet gel extraction kit and following its protocol.
• Concentrations of DNA were measured by nanodrop
• Sample B (Tod C gene) was not purified correctly, another PCR is needed. (Nanodrop shows contamination)
• Table representing the concentration and volume of DNA isolated from samples

Sample	Volume	Concentration ng/ul	260/280	260/230
A	48	19.3	2.02	0.03
C	50	27.7	1.88	0.04
D	49	26.9	1.71	0.05
E	50	20.5	1.98	0.03