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- Phage Purification
- March-April
- May-June
- July-August
- September-October
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6.5 Bacteria Cultures
I) Purpose
- Grow bacteria for later use in preparation of phage.
II) Expected Outcome
- Viable liquid cultures of both W3110 and BL21
III) Reagants Used
- W3110 and BL21 freezer stock
- LB
IV) Actual Procedure
- Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37◦ C for 1 hour.
- Infect 4 mL of LB with 1 mL of liquid culture prepared in previous step.
- Let both liquid cultures sit overnight at 37◦ C.
V) Results
- We were able to grow viable cultures of W3110 and BL21.
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