Team:EPF Lausanne/Calendar/16 August 2013


Taxi.Coli: Smart Drug Delivery iGEM EPFL


Cell Surface Display

BBa_K523013 Characterisation
Characterisation of the iGEM plasmid BBa_K523013 we decided to use.

Making a glycerol stock
-From each culture that was inoculated we made a 50% glycerol stock of 500ul Bacterial culture and 500ul 50% glycerol.

We Started the Gibson assembly of four of our constructs by making a PCR of each, the Insert as well as the Backbone, making eight different PCR tubes in total (see protocol PCR). About twenty minutes before the PCR reaction was done, the machine malfunctioned. We did run a 0.8% gel, but The results cannot be well interpreted, as the reaction could not go to completion. We re-ran another PCR reaction with the same constructs, as well as a reactant control. After the PCR the amplified DNA was stored over night at 4°C.


Making Nanoparticles, 2nd try
- We washed the nanoparticles with 75% acetone.