Team:Paris Bettencourt/Notebook/Phage Sensor/Sunday 22nd September.html
From 2013.igem.org
Phage Sensor
ASDFSunday 22nd September
PCR and Gels
PCRM13 backbone
SPCR16,17
Reagent | Volume |
x1 | |
Nuclease-free water | 37.25 µl |
5x Phusion HF Buffer | 10 µl |
10 mM dNTPs | 1 µl |
Forward Primer (10 uM) | 0.5 µl |
Reverse Primer (10 uM) | 0.5 µl |
Template Plasmid | 0.25 µl |
Phusion DNA Polymerase | 0.5 µl |
Total Volume | 50 µl |
Protocol see fotos
Gel 100V, 1%, 1,5h