Team:Paris Bettencourt/Notebook/Week4
From 2013.igem.org
Contents |
Week 4
Monday 24th June
Tuesday 25th June
Wednesday 26th June
Thursday 27th June
Friday 28th June
Trojan Horse
sRNA design
Red sequences:
pACYCDuet-1 (CmR) : ATATCCAGTGATTTTTTTCTCCAT
pCOLADuet-1 (KanR) : CGTTTCCCGTTGAATATGGCTCAT
We decided that targeting only two different antibiotic resistance will be enough for a proof of concept. We give up AmpR because otherwise they might be experimental issues with AmpR that is also on phagemid litmus28i.