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Team:TU-Delft/Notebook/2013/07/09/
From 2013.igem.org
Notebook
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9th of July
Lab work
1. The agar plates of pBAD, pT7, GFP, AIP sender(Agr A: AgrC). AIP receiver(AgrB: AgrD), pTET:lysis didnot give convincing results. So it was discarded.
2. We start afresh to make the timer from iGEM 2009.
3. List of transformations:
| Part | BBa_ | Backbone | Resistance | Distribution | Plate | Well |
| pBAD:araC:RBS:agrB:RBS:agrD:TT | BBa_I746008 | pSB2K3 | Kanamycin | Requested part from iGEM HQ | - | - |
| RBS:AgrC:RBS:AgrA:TT:pP2 | BBa_I746103 | pSB1A2 | Ampicillin | Requested part from iGEM HQ | - | - |
| RBS:GFP:TT | BBa_E0240 | pSB1C3 | Choloramphenicol | Distribution kit | 3 | 23 A |
| pTET:RBS | BBa_J13002 | pSB1A2 | Ampicillin | iGEM TuDelft 2009 | - | - |
| cI | BBa_C0051 | pSB1A2 | Ampicillin | iGEM TuDelft 2009 | - | - |
| TT | BBa_B0015 | pSB1AK3 | Kanamycin+Ampicillin | iGEM TuDelft 2009 | - | - |
| RBS:TetR:TT | BBa_P0440 | pSB1A2 | Ampicillin | iGEM TuDelft 2009 | - | - |
| RBS:RFP:TT | BBa_I13507 | pSB1A2 | Ampicillin | iGEM TuDelft 2009 | - | - |
All transformations were streaked on agar plates with corresponding antibiotic resistance.
4. Streaked the pBAD - 10% and 90%, pT7 - 10% and 90% on Agar plates.
5. Did short experiment to check if the first attempt of pTET:lysis worked. Added Tetracycline to pTET:lysis in concentrations of 1% and 10%. Would check results tomorrow.
