LOCUS       pKH4                    6511 bp    CIRCULAR             01-SEP-2013
UNIMARK     pKH4 annotations
            pKH4
FEATURES             Location/Qualifiers
     source          1..6511
                     /comment="Molecule is created with Unipro UGENE v1.12"
     misc_feature    996..2459
                     /ugene_name="pSB1C3-RBS1_p"
     misc_feature    complement(2434..2468)
                     /mismatches=0
                     /ugene_name="RB22_rv"
     misc_feature    2438..2491
                     /mismatches=0
                     /ugene_name="RB27_fw"
     misc_feature    2448..2459
                     /ugene_name="BBa_B0034"
     misc_feature    2466..6323
                     /ugene_name="indC-Plum"
     misc_feature    complement(5285..5315)
                     /mismatches=0
                     /ugene_name="KH4"
     misc_feature    5293..5315
                     /mismatches=0
                     /ugene_name="KH1"
     misc_feature    5316..5504
                     /ugene_name="T-Domain"
     misc_feature    complement(5505..5528)
                     /mismatches=0
                     /ugene_name="KH2"
     misc_feature    5505..5533
                     /mismatches=0
                     /ugene_name="KH3"
     misc_feature    6324..6338
                     /ugene_name="BBa_B0029"
     RBS             complement(6358..6363)
                     /vntifkey=32
                     /label="RBS"
     protein_bind    complement(6394..6414)
                     /vntifkey=31
                     /label="lacO"
     promoter        complement(6419..6435)
                     /vntifkey=30
                     /label="T7\promoter"
     primer          complement(6441..6460)
                     /vntifkey=27
                     /label="DuetUP2\Primer\#71180-3"
     primer          6441..6460
                     /vntifkey=27
                     /label="DuetDOWN1\Primer\#71179-3"
ORIGIN
        1 CTAATAACGC TGATAGTGCT AGTGTAGATC GCTACTAGAG CCAGGCATCA AATAAAACGA
       61 AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC
      121 TACTAGAGTC ACACTGGCTC ACCTTCGGGT GGGCCTTTCT GCGTTTATAT ACTAGTAGCG
      181 GCCGCTGCAG TCCGGCAAAA AAGGGCAAGG TGTCACCACC CTGCCCTTTT TCTTTAAAAC
      241 CGAAAAGATT ACTTCGCGTT ATGCAGGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG
      301 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT
      361 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA
      421 AAAAGGCCGC GTTGCTGGCG TTTTTCCACA GGCTCCGCCC CCCTGACGAG CATCACAAAA
      481 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC
      541 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT
      601 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
      661 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG
      721 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
      781 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA
      841 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT
      901 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
      961 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA
     1021 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
     1081 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT
     1141 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA
     1201 GCTCGAGGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAACA
     1261 AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA AGCGAGCTCG
     1321 ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT
     1381 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC
     1441 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC
     1501 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA
     1561 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA
     1621 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT
     1681 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC
     1741 ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT CAGGCGGGCA
     1801 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG
     1861 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC
     1921 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT
     1981 TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT
     2041 AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCCGAT CAACTCGAGT
     2101 GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT
     2161 CACGAGGCAG AATTTCAGAT AAAAAAAATC CTTAGCTTTC GCTAAGGATG ATTTCTGGAA
     2221 TTCGCGGCCG CTTCTAGAGC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT
     2281 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT
     2341 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT
     2401 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAT ACTAGAGAAA GAGGAGAAAG
     2461 GTACCATGTT AGAAAATAAT ATTACACAAT GTGACTCAAT CAATGATGTT TATCTTAAAG
     2521 AAGAAGCAAT AACATTGATG GATATGCTTG AGAGTCAACT TAAGCACCAG GCAGATGGAT
     2581 ATGTTGTTAT TGATCAAGAA GAATCTCTCA GTTACGCTGA TTTCTATTTG AGGGTGAAAG
     2641 AGATAGGGTA TTGTCTGTCA GAAATTAGCT CAAAGAATTC GGTGGGTATT GGGCTTTTTT
     2701 GTGATCCTTC TATAGATTTA ATTTGTGGTG CATGGGGTAT TTTGTCAGCG GATAAAGCTT
     2761 ATTTGCCGTT ATCGCCTGAC TATCCAACTG AACGCCTCAA ATATATGATA GAAGATTCTG
     2821 GTATTGATGT GATTTTTACG CAATCGCACT TAAAAGCACA GCTACAGGAC ATTGCACCAA
     2881 AATCAGTATT AATTATGACA CCAGAAGATG TCGCTCTGAC GATAAAAACA CGAACAATAG
     2941 AAGATATTCT GGGCACAGTT CAAGTTCCTA AACCCACTAG TCTGGCTTAT ATTATTTATA
     3001 CCTCTGGTAG CACGGGTAAG CCAAAGGGAG TGATGATTGA ACATCACAGT ATTGTAAATC
     3061 AAATGAGATT TCTTGCAAAA GCGTTCAAAT TAGGATGTCA TTCCCGGATT TTACAGAAAA
     3121 CACCAATGAG TTTTGATGCG GCTCAATGGG AAATTCTAGC GCCTGCAATT GGTGGTCAAG
     3181 TGATTATGGG TCCTTTAGGT TGCTATCGCG ATCCGGATGC AATTATTAAA ACCATTCTTC
     3241 AGCATCAAGT AACGACTTTG CAATGTGTTC CTACTTTGCT ACAAGCGTTA CTGGATAATC
     3301 CTAATTTTTT GGATTGCTTA TCATTGACTC AAGTATTCAG TGGGGGAGAA GCGCTGACAA
     3361 CCAAATTAGC CACGCAATTT TTGAATAGTT TTACTCACTG TGAATTAATC AATTTATATG
     3421 GCCCGACAGA ATGTACGATT AATTCATCAT TTTTCCGGGT GACAAATGAG ACTTTGCCGA
     3481 ATTATCAAAC CTCTATTTCG ATTGGTGCAC CTGTAGATAA TACCGAATAC TACGTTCTTG
     3541 ATGATGATAG ATTACCTGTG GCGGTTGGCG AAATTGGCGA GCTTTATATT TCGGGTGCTC
     3601 AATTAGCACG TGGTTATTTG CATAAACCAG AAATGACAAA AGATAAATTT ATTTGTAATC
     3661 ACCTTGTATC AGGAACTCAA CATCAATGGT TATATCGAAC GGGAGATCTG GTAACCAGAG
     3721 GGGCTGATGG TAATACTTAT TTTGTTGGTC GGGTTGATAG CCAGGTCAAA TTACGAGGTT
     3781 ACCGTATTGA GCTTGATGAA ATACGCCATG CGATTGAAGA ACATAGCTGG ATAAAGACGG
     3841 CGGCAATGTT AATTAAGAAG GATGCCAGAA CGGGTTTCCA AAATCTCATC GCGTGTGTGG
     3901 AATTAGATGA GAAAGAAGCT GCATTGATGG ATCAAGGTAA TAGTAGCTCA CATCACAAAT
     3961 CAAAAGCCGA TAAACTACAG GTGAAAGCCC AACTTTCTAA TTCTGGTTGT CGAAGTGAAG
     4021 AGTTATGTGA AAATCGCCCT ACATTCTTAC TTCCTTATCA AGAAGGGGAG ATAAAACAGA
     4081 GAGAATATGC ATTTGGACGC AAGACATATC GCTATTTTGA GGGAACAGAA ATAACGGTAG
     4141 AGAAATTAAA AAAATTGCTG ACAGCCACTC AATCGAATGA AATTAGCTCT TTGCCACTGA
     4201 GTCATCTAAC CCTGAATGAT TTCGGTTATG CATTGCGTTA TTTTGGTCAG TTTACCAGCC
     4261 ATCAACGTTT ATTGCCCAAA TATGCCTATG CTTCACCGGG TGCTCTCTAT GCGACACAAA
     4321 TGTATTTTGA ATTGCATAAT GTTCTCGGTT TGGATGCGGG GATTTACTAT TATCATCCAG
     4381 TGACACATAA GTTAATAAAA ATTTCAACAT TGAGTCGTCG GCAAATGCCA ACGATAAAAG
     4441 TGCATTTTAT TGGCAAGCAT GAAGCCATTG AGCCCGTTTA TAAGAACAAT ATACAAGAAG
     4501 TTCTGGAAAT GGAAGCGGGC CATATGATGG GTCTTTTTGA TGACGTATTA CCGGAAATTG
     4561 GCTTGAGTAT TGGTAAAAGT GAATATCAAG ATGAATGTCC AGATTGGTAT GATGGTGATA
     4621 TTCAGGATTA TTATCTTGGT GCATTTGAAA TATGTAGCTA TGAACATGGA TTGCCGCCAT
     4681 TTGAGACTGA TATTTATTTA CAAACACATG CCCATAAAAT ACCTGAGATG CCGTGTGGTT
     4741 TATATCACTT TTCTAACGGG GAATTTGTAC GAATAAGTGA TGATATTGTC CGAAAAAAGG
     4801 ATGTTATTGC GATTAATCAG CAAGTTTATG ATCGCTCCAG TTTTGGCGTG TCAATTATTC
     4861 CACGCTGTGT CCCTGAATGG CATTATTATA TAACACTGGG TCGTCGGTTA CATGCGTTAC
     4921 AAAGTAATCC ATTGTATATT GGATTAATGT CATCTGGTTA CAGTTCGAAG AGCAATAACG
     4981 ATTTACCTTC GGCGAAAAGG ATGCGATCTA TTCTCAATGC ACTTGATAGA CCTATGGCGG
     5041 CATTTTATTT CTGCATAGGT GGGGGTATTA GCCAAGCGCA ATATATGTGT GAAGGCATGA
     5101 AAGAAGATGT TGTTCATATG AAAGGGCCAG TTGAAATCAT TAAAGATGAT CTTCAACAAC
     5161 AACTCCCTCA ATATATGATT CCAAATAAGG TATTAGTTTT CGATAAATTA CCTTTGACGG
     5221 CCAATGGAAA AGTGGATTAT CAATCTTTAT CAGAATCTAA AGCCGTGGAG AATGTTTCAA
     5281 CACAGCGTCT ATTGGTGCCA TTACATACAG ATACTGAAAT AAGGCTTGGA AAAATTTGGA
     5341 TGGAAGTACT GAAATGGGAT TCAGTATCTG CCCTCGATGA TTTTTTCGAA AGTGGGGGTA
     5401 ATTCTTTGAT GGCCGTTGCA ATGGTTAATA AGATCAATGC GGCCTTTAAT ATTCGTTTTC
     5461 CGTTACAGAT ACTTTTTCAA TCTCCTAATA TAGCAGAATT GGCTAAGTGG ATTGAACAGA
     5521 CAGACTCTAA AACAATATCA AGATTAATTT TATTGAATCA GGCAAGCAAA GACCCCATTT
     5581 ACTGTTGGCC GGGTTTGGGC GGATATCCTA TGAGTTTGAG ATTGCTTGCT AATAAAGTCG
     5641 TTCCTGATCG GGCATTTTAT GGAATACAGG CATATGGGAT AAACGAGAGT GAAATACCGT
     5701 TTTCTTCTAT CCAGAGAATG GCAGAAGAGG ATATTAAAGA GATAAAGAAA ATACAGCCAG
     5761 AAGGGCCATA TATATTGTGG GGATATTCAT TTGGTGCCCG AGTAGCATTT GAAGTTGCAT
     5821 ACCAGCTTGA ACAAGCGGGA GAAGAAGTTA ACGCATTGAA TTTATTGGCT CCGGGATCTC
     5881 CTCATCTTGA TATGAAGCAA GCGGAATATA TGGATAAAGG CGCTGAATTT ACTAATCCGG
     5941 CTTTTGTTAA AATACTTTTT TCTGTATTTT CTCGTTCAAT CAACAGCCCA ATGGTTAAAA
     6001 CTTGCTTAGA ACAAGTAAAT AGTGAAACGA CATTTATTAA CTTTATATGT AGTCGTTTTA
     6061 AAAACTTGGA ACCATCATTA GTAAAACGTA TCGTTAGGAT TGTGACTTTG ACTTATGATT
     6121 TCAAGTACAG TATTGATGAG CTTTATCACA GACACCTAAA GGCACCTATA ACTATTTTCA
     6181 AGGCGAATAG AGATAATGAT TCATTTATCG AGGAATCGGA TGTGATTTCA TCAATGTCGC
     6241 CTAAAATAAT TGAATTAATA TCGGATCACT ATCAACTGTT GGAAAGTGAA GGTGTTGCTG
     6301 AGATTGAGAA AATAATCTAA TAATTCACAC AGGAAACCGG ATCTGCCATA TGTATATCTC
     6361 CTTCTTATAC TTAACTAATA TACTAAGATG GGGAATTGTT ATCCGCTCAC AATTCCCCTA
     6421 TAGTGAGTCG TATTAATTTC GATTATGCGG CCGTGTACAA TACGATTACT TTCTGTTCGA
     6481 CTTAAGCATT ATGCGGCCGC AAGCTTTCTA G
//
