"
Team:ETH Zurich/Experiments
From 2013.igem.org
(Difference between revisions)
| Line 71: | Line 71: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Mutagenized Plux <br> | + | <td>Mutagenized Plux <br>PLuxR1</td> |
<td>Optimized Biobrick !</td> | <td>Optimized Biobrick !</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Mutagenized Plux <br> | + | <td>Mutagenized Plux <br>PLuxR2</td> |
<td>Optimized Biobrick !</td> | <td>Optimized Biobrick !</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Mutagenized Plux <br> | + | <td>Mutagenized Plux <br>PLuxR3</td> |
<td>Optimized Biobrick !</td> | <td>Optimized Biobrick !</td> | ||
</tr> | </tr> | ||
Revision as of 08:24, 3 September 2013
Tranformation and cloning constructs
| Sender cells | Receiver cells | |
|---|---|---|
| AHL expressing system | Fluorescent reporter system | Enzyme reaction-based reporter system |
| Pcons-LuxI 1 (Strong) | Plac-LuxR-Plux-GFP | Plac-LuxR-Plux-LacZ |
| Pcons-LuxI 2 | Plac-LuxR-Plux-YFP | Plac-LuxR-Plux-Aes |
| Pcons-LuxI 3 | Plac-LuxR-Plux-CFP | Plac-LuxR-Plux-GusA |
| Pcons-LuxI 4 (Weak) | Plac-LuxR-Plux-PhoA | |
| Plac-LuxR-Plux-NagZ | ||
| Biobrick | Registry part |
|---|---|
| LuxI | K805016 |
| LuxR | C0062 |
| Pcons 1 (Strong) | J23100 |
| Pcons 2 | J23118 |
| Pcons 3 | J23110 |
| Pcons 4 (Weak) | J23114 |
| Mutagenized Plux PLuxR1 |
Optimized Biobrick ! |
| Mutagenized Plux PLuxR2 |
Optimized Biobrick ! |
| Mutagenized Plux PLuxR3 |
Optimized Biobrick ! |
| pLuxR | R0062 |
| Plac | I14032 |
| GFP | E0840 |
| YFP | E0030 |
| CFP | E0020 |
| LacZ | I732006 | GusA | New Biobrick ! |
| PhoA | New Biobrick ! |
| Aes | New Biobrick ! |
| NagZ | New Biobrick ! |
Final constructs
