Team:ETH Zurich/Experiments

From 2013.igem.org

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<td>Mutagenized Plux <br>PLuxR1</td>
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<td>pLuxR</td>
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<td>Optimized Biobrick !</td>
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<td>R0062</td>
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<td>Mutagenized Plux <br>PLuxR2</td>
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<td>Mutagenized PLuxR_1</td>
<td>Optimized Biobrick !</td>
<td>Optimized Biobrick !</td>
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</tr>
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<td>Mutagenized Plux <br>PLuxR3</td>
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<td>Mutagenized PLuxR_2</td>
<td>Optimized Biobrick !</td>
<td>Optimized Biobrick !</td>
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<td>pLuxR</td>
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<td>Mutagenized PLuxR_3</td>
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<td>R0062</td>
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<td>Optimized Biobrick !</td>
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<h1><b>Final constructs</b></h1>
<h1><b>Final constructs</b></h1>
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[[File:Constructs.png|1000px]]
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[[File:Constructs.png|1000px|Thumb|<b>Figure 1. Transformed plasmids for mine cells and non-mine cells </b>]]
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Revision as of 08:27, 3 September 2013

Header2.png
80px-Eth igem logo.png

Tranformation and cloning constructs

Sender cells Receiver cells
AHL expressing system Fluorescent reporter system Enzyme reaction-based reporter system
Pcons-LuxI 1 (Strong) Plac-LuxR-Plux-GFP Plac-LuxR-Plux-LacZ
Pcons-LuxI 2 Plac-LuxR-Plux-YFP Plac-LuxR-Plux-Aes
Pcons-LuxI 3 Plac-LuxR-Plux-CFP Plac-LuxR-Plux-GusA
Pcons-LuxI 4 (Weak) Plac-LuxR-Plux-PhoA
Plac-LuxR-Plux-NagZ
Biobrick Registry part
LuxI K805016
LuxR C0062
Pcons 1 (Strong) J23100
Pcons 2 J23118
Pcons 3 J23110
Pcons 4 (Weak) J23114
pLuxR R0062
Mutagenized PLuxR_1 Optimized Biobrick !
Mutagenized PLuxR_2 Optimized Biobrick !
Mutagenized PLuxR_3 Optimized Biobrick !
Plac I14032
GFP E0840
YFP E0030
CFP E0020
LacZ I732006
GusA New Biobrick !
PhoA New Biobrick !
Aes New Biobrick !
NagZ New Biobrick !












Final constructs

Figure 1. Transformed plasmids for mine cells and non-mine cells