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Team:Bielefeld-Germany/Labjournal/August
From 2013.igem.org
(Difference between revisions)
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<h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Cytochromes">Cytochromes</a></h4> | <h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/Project/Cytochromes">Cytochromes</a></h4> | ||
| - | <p></p> | + | <div class="cyt_restr1_hl"><p><a>Restriction of ccmAH and psB1C3 and Suffix-Insertion</a></p></div> |
| + | <div class="cyt_retr1"> | ||
| + | <blockquote> | ||
| + | The backbone and shipping vector psB1C3 was cut with EcoRI, SpeI, the insert ccmAH with EcoRI and XbaI to perfom a standard suffix insertion. | ||
| + | <br>We used approx. 200ng insert and 42ng vector, which repressnts a 2-fold molar excess of insert. | ||
| + | <br>The Ligation was incubated for 15 min at 37°C, followed by a heat-inactivation at 80°C for 20 min. | ||
| + | <br>The subsequent gel electrophoresis did not yield any positiv results, probably due to the use of buffer and enzymes from two different manufactures. | ||
| + | <br> | ||
| + | </blockquote> | ||
| + | </div> | ||
| + | |||
| + | |||
<h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/BioSafety">Biosafety</a></h4> | <h4><a href="https://2013.igem.org/Team:Bielefeld-Germany/BioSafety">Biosafety</a></h4> | ||
Revision as of 10:54, 3 September 2013
August
Milestones
1.Week
MFC
Mediators
Cytochromes
The backbone and shipping vector psB1C3 was cut with EcoRI, SpeI, the insert ccmAH with EcoRI and XbaI to perfom a standard suffix insertion.
We used approx. 200ng insert and 42ng vector, which repressnts a 2-fold molar excess of insert.
The Ligation was incubated for 15 min at 37°C, followed by a heat-inactivation at 80°C for 20 min.
The subsequent gel electrophoresis did not yield any positiv results, probably due to the use of buffer and enzymes from two different manufactures.
