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Team:ETH Zurich/Experiments
From 2013.igem.org
(Difference between revisions)
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<td>I732006</td> | <td>I732006</td> | ||
</tr> | </tr> | ||
| - | |||
| - | |||
<tr> | <tr> | ||
<td>[https://2013.igem.org/Team:ETH_Zurich/Experiments_3 PhoA]</td> | <td>[https://2013.igem.org/Team:ETH_Zurich/Experiments_3 PhoA]</td> | ||
| - | <td> | + | <td>J61032</td> |
| + | </tr> | ||
| + | <tr> | ||
| + | <td>[https://2013.igem.org/Team:ETH_Zurich/Experiments_3 GusA]</td> | ||
| + | <td>K330002</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 118: | Line 120: | ||
<tr> | <tr> | ||
<td>[https://2013.igem.org/Team:ETH_Zurich/Experiments_3 NagZ]</td> | <td>[https://2013.igem.org/Team:ETH_Zurich/Experiments_3 NagZ]</td> | ||
| + | <td>New Biobrick !</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>PhoA with His-Tag</td> | ||
| + | <td>New Biobrick !</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>GusA with His-Tag</td> | ||
| + | <td>New Biobrick !</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> Aes with His-Tag</td> | ||
<td>New Biobrick !</td> | <td>New Biobrick !</td> | ||
</tr> | </tr> | ||
Revision as of 08:21, 6 September 2013
Cloned constructs
| Sender cells | Receiver cells | |
|---|---|---|
| AHL expressing system | Fluorescent reporter system | Enzyme reaction-based reporter system |
| Pcons-LuxI 1 (Strong) | Plac-LuxR-Plux-GFP | Plac-LuxR-Plux-LacZ |
| Pcons-LuxI 2 | Plac-LuxR-Plux-YFP | Plac-LuxR-Plux-Aes |
| Pcons-LuxI 3 | Plac-LuxR-Plux-CFP | Plac-LuxR-Plux-GusA |
| Pcons-LuxI 4 (Weak) | Plac-LuxR-Plux-PhoA | |
| Plac-LuxR-Plux-NagZ | ||
| Biobrick | Registry part |
|---|---|
| LuxI | K805016 |
| LuxR | C0062 |
| Pcons 1 (Strong) | J23100 |
| Pcons 2 | J23118 |
| Pcons 3 | J23110 |
| Pcons 4 (Weak) | J23114 |
| pLuxR | R0062 |
| Mutagenized PLuxR_1 | Optimized Biobrick ! |
| Mutagenized PLuxR_2 | Optimized Biobrick ! |
| Mutagenized PLuxR_3 | Optimized Biobrick ! |
| Plac | I14032 |
| GFP | E0840 |
| YFP | E0030 |
| CFP | E0020 |
| LacZ | I732006 |
| PhoA | J61032 |
| GusA | K330002 |
| Aes | New Biobrick ! |
| NagZ | New Biobrick ! |
| PhoA with His-Tag | New Biobrick ! |
| GusA with His-Tag | New Biobrick ! |
| Aes with His-Tag | New Biobrick ! |
Final constructs
