Team:BYU Provo/Notebook/SmallPhage/Fallexp/Period1/Dailylog

From 2013.igem.org

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<font size="4"> '''8/22/13''' </font>
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* Nothing showed on the S1-S12 and B1-B12 plates. Wildtype had too many plaques (T7 at -4).
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JL, LJP
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* T7 propagation for [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.26 Mutagen Concentration Test|8.26 Mutagen Concentration Test - Eighth Protocol]].  
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* Purified phage propagation sample from 9.4 via centrifugation (5 minutes at 3000 rpm) and cholorform. Spot tests were then performed to estimate phage titer.
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* Started approximately 20 mL of E coli liquid culture overnight.
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* Took pictures of plates from [[Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size|8.16 Modeling phage plaque size - Experiment One]] using alpha imager
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* Divided up assignments for updating notebook.
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* Divided up
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Revision as of 22:53, 6 September 2013


Small Phage September - October Notebook: September 1 - September 15 Daily Log



Small Phage
March-April
May-June
July-August
September-October

9/1/13

  • Started approximately 10mL of E coli B liquid culture overnight.


9/2/13


9/3/13

  • Started approximately 20 mL of E coli B liquid culture overnight.


9/4/13

JL, LJP

  • Discussed results from recent spot test for 8.26 Mutagen Concentration Test - Eighth Protocol. Phage too spread out to effectively select for mutant phage. Decided to stop until the Phage Purification Team can get the wildtype T7 phage to band.
  • Discussed plans and designs for team wiki with Darren and Keltzie.
  • Started T7 propagation to generate enough phage for the Phage Purification Team to experiment with. Specifically, to a 15mL centrifuge tube we added 8mL of LB, 2mL of E coli B liquid culture overnight, and 20uL of 8.24 T7 phage stock.


9/5/13

LJP

  • Started approximately 10mL of E coli B liquid culture overnight.


9/6/13

JL, LJP

  • Purified phage propagation sample from 9.4 via centrifugation (5 minutes at 3000 rpm) and cholorform. Spot tests were then performed to estimate phage titer.

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  • Divided up assignments for updating notebook.
  • Divided up


8/23/13

- Repeated plating of S1-S12 and B1-B12 in 8.14 Mutagen Concentration Test - Seventh Protocol.


8/24/13

- Still no bacteria or phage plaques showed up for S1-S12 and B1-B12 in 8.14 Mutagen Concentration Test - Seventh Protocol -> We decided to focus on 8.26 Mutagen Concentration Test - Eighth Protocol instead

- Purified T7 phage propagation and performed a spot test to estimate the titer of this fresh stock (8.24).

- Started approximately 10mL of E coli B liquid culture overnight


8/25/13

- Titer was performed for T7 phage 8.24 stock at -6 and -7 to gain a more accurate estimate of phage concentration.

- Started approximately 10mL of E coli B liquid culture overnight.


8/26/13

- Performed the actual mutagenesis procedure in 8.26 Mutagen Concentration Test - Eighth Protocol.

- Phage Purification Team was able to run the CsCl gradient right after the completion of mutagenesis.

- Started approximately 20mL of E coli B liquid culture overnight.


8/27/13

- Spot test was performed for the post CsCl aliquots (for both control and mutated group) to estimate phage concentration in each. For specific procedure please refer to 8.26 Mutagen Concentration Test - Eighth Protocol.


8/30/13

- Started approximately 20mL of E coli B liquid culture overnight.

- Worked on editing the abstract for submission.


8/31/13

- Further dilution series and spot test was performed to gain a more accurate idea of phage distribution in CsCl gradient. Specific procedures can be found at 8.26 Mutagen Concentration Test - Eighth Protocol.

- Started approximately 10mL of E coli B liquid culture overnight.