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Team:UNITN-Trento/Notebook/Labposts/06/52
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(Created page with "{ "date" : "2013-06-20", "author" : "emil", "title" : "Linearization of psB1C3 (Failed)", "content" : "<html>I have re-tried to linearize through a PCR the backbone PsB1C3 th...") |
Revision as of 07:46, 9 September 2013
{
"date" : "2013-06-20",
"author" : "emil",
"title" : "Linearization of psB1C3 (Failed)",
"content" : "I have re-tried to linearize through a PCR the backbone PsB1C3 that we have previously purified and quntified.
I have tried to amplify the sample of 117.3 ng/µl, 105.7 ng/µl and one of Girelli's sample(184.1 ng/µl)following the Phusion PCR protocol and the universal primers.
No one of this PCRs has succeded!
Linearization attempt 2width=\"450px\" />
",
"tags" : "pSB1C3"
}
