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Team:ETH Zurich/Experiments
From 2013.igem.org
(Difference between revisions)
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<table style="float:left;margin-top:10px;width:auto;height:auto;font-size:18px"> | <table style="float:left;margin-top:10px;width:auto;height:auto;font-size:18px"> | ||
<tr> | <tr> | ||
| - | <th width="500px">Sender cells/ | + | <th width="500px" height="50px">Sender cells / OHHL expressing systems</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Pcons-LuxI 1 (Strong)</td> | + | <td>Pcons-LuxI (1) (Strong)</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Pcons-LuxI 2 </td> | + | <td>Pcons-LuxI (2) </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Pcons-LuxI 3 </td> | + | <td>Pcons-LuxI (3) </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Pcons-LuxI 4 (Weak) </td> | + | <td>Pcons-LuxI (4) (Weak) </td> |
</tr> | </tr> | ||
</table> | </table> | ||
<table style="float:right;margin-top:10px;width:auto;height:auto;font-size:18px"> | <table style="float:right;margin-top:10px;width:auto;height:auto;font-size:18px"> | ||
| - | <th colspan="2">Receiver cells</th> | + | <th colspan="2" height="50px">Receiver cells / OHHL detecting and processing systems</th> |
<tr> | <tr> | ||
| - | <th width="320px">Fluorescent reporter system</th> | + | <th width="320px" height="50px">Fluorescent reporter system</th> |
| - | <th width="500px">Enzyme reaction-based reporter system</th> | + | <th width="500px" height="50px">Enzyme reaction-based reporter system</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<table style="float:left;margin-top:10px;height:auto;font-size:18px;cellspacing:2px"> | <table style="float:left;margin-top:10px;height:auto;font-size:18px;cellspacing:2px"> | ||
<tr> | <tr> | ||
| - | <th>Biobrick</th> | + | <th height="50px" width="130px" >Biobrick</th> |
| - | <th>Registry part</th> | + | <th height="50px" width="120px" >Registry part</th> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 69: | Line 69: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Pcons 1 (Strong)</td> | + | <td>Pcons. (1) (Strong)</td> |
<td>J23100</td> | <td>J23100</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Pcons 2 </td> | + | <td>Pcons. (2) </td> |
<td>J23118</td> | <td>J23118</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Pcons 3 </td> | + | <td>Pcons. (3) </td> |
<td>J23110</td> | <td>J23110</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Pcons 4 (Weak) </td> | + | <td>Pcons. (4) (Weak) </td> |
<td>J23114</td> | <td>J23114</td> | ||
</tr> | </tr> | ||
Revision as of 08:37, 10 September 2013
Cloned constructs
| Sender cells / OHHL expressing systems |
|---|
| Pcons-LuxI (1) (Strong) |
| Pcons-LuxI (2) |
| Pcons-LuxI (3) |
| Pcons-LuxI (4) (Weak) |
| Receiver cells / OHHL detecting and processing systems | |
|---|---|
| Fluorescent reporter system | Enzyme reaction-based reporter system |
| Plac-LuxR-PluxR-GFP | Plac-LuxR-PluxR-LacZ |
| Plac-LuxR-PluxR-YFP | Plac-LuxR-PluxR-Aes |
| Plac-LuxR-PluxR-CFP | Plac-LuxR-PluxR-GusA |
| Plac-LuxR-PluxR-PhoA | |
| Plac-LuxR-PluxR-NagZ |
Final constructs
| Biobrick | Registry part |
|---|---|
| LuxI | K805016 |
| LuxR | C0062 |
| Pcons. (1) (Strong) | J23100 |
| Pcons. (2) | J23118 |
| Pcons. (3) | J23110 |
| Pcons. (4) (Weak) | J23114 |
| pLuxR | R0062 |
| Mutagenized PLuxR_1 | Optimized Biobrick ! |
| Mutagenized PLuxR_2 | Optimized Biobrick ! |
| Mutagenized PLuxR_3 | Optimized Biobrick ! |
| Plac | I14032 |
| GFP | E0840 |
| YFP | E0030 |
| CFP | E0020 |
| LacZ | I732006 |
| PhoA | J61032 |
| GusA | K330002 |
| Aes | New Biobrick ! |
| NagZ | New Biobrick ! |
| PhoA with His-Tag | Optimized Biobrick ! |
| GusA with His-Tag | Optimized Biobrick ! |
| Aes with His-Tag | Optimized Biobrick ! |
